LOCUS       sequence-254993-       11605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A self-assembling split-GFP based Golden Gate vector to determine 
            protein targeting to mitochondria in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 11605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pMiNGo11-3
FEATURES             Location/Qualifiers
     source          1..11605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1030..1375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1769..2410
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      2441..3148
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4165..4510
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4640..4945)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4742..4761)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5265..5948)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     5863..5882
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5949..6051)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6082..6129
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6145..6192
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6208..6255
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    6963..6987
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7267..7896
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8325..9398
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9464..9658
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9720..9824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9825..10619
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10790..11378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11279..11298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11514..11538
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gaattccaat cccacaaaaa
       61 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
      121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
      181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
      241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
      301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
      361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
      421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
      481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
      541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
      601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
      661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
      721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
      781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
      841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
      901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
      961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     1021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     1081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     1141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     1201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     1261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     1321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     1381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1441 aattacattt acaattatcg ataccatgct tcgagtagca ggtagaaggc tttcttcttc
     1501 agccgctaga tcttcatcta ccttctttac aagaagctct ttcaccgtta ccgatgattc
     1561 gtctccggca agatctcctt ctccgtcact cacctcttcg tttctcgatc aaatcagagg
     1621 tttctcatct aattcggttt ctcccgcaca tcagttgggt ttagtctcag atcttccagc
     1681 cacagtggct gctattaaga atcccagttc aaaaattgta tatgatgact ccaaccatga
     1741 gcgttatcca cctggagatc ctagcgcaat gtccaaagga gaagaactgt ttaccggtgt
     1801 tgtgccaatt ttggttgaac tcgatggtga tgtcaacgga cataagttct cagtgagagg
     1861 cgaaggagaa ggtgacgcca ccattggaaa attgactctt aaattcatct gtactactgg
     1921 taaacttcct gtaccatggc cgactctcgt aacaacgctt acgtacggag ttcagtgctt
     1981 ttcgagatac ccagaccata tgaaaagaca tgactttttt aagtcggcta tgcctgaagg
     2041 ttacgtgcaa gaaagaacaa tttcgttcaa agatgatgga aaatataaaa ctagagcagt
     2101 tgttaaattt gaaggagata ctttggttaa ccgcattgaa ctgaaaggaa cagattttaa
     2161 agaagatggt aatattcttg gacacaaact cgaatacaat tttaatagtc ataacgtata
     2221 catcactgct gataagcaaa agaacggaat taaagcgaat ttcacagtac gccataatgt
     2281 agaagatggc agtgttcaac ttgccgacca ttaccaacaa aacaccccta ttggagacgg
     2341 tccggtactt cttcctgata atcactacct ctcaacacaa acagtcctga gcaaagatcc
     2401 aaatgaaaaa ggaacaggtg gcggcggaag ttagcttgtc ctgctttaat gagatatgcg
     2461 agaagcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
     2521 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
     2581 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact
     2641 acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac
     2701 atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt
     2761 aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt
     2821 tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca
     2881 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
     2941 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
     3001 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
     3061 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
     3121 tttgcgacaa catgtcgagg ctcagcagga ccgctactag aattcgagct cggaggaatt
     3181 ccaatcccac aaaaatctga gcttaacagc acagttgctc ctctcagagc agaatcgggt
     3241 attcaacacc ctcatatcaa ctactacgtt gtgtataacg gtccacatgc cggtatatac
     3301 gatgactggg gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc acacaagaaa
     3361 tttgccacta ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca
     3421 gacaggttga acttcatccc caaaggagaa gctcaactca agcccaagag ctttgctaag
     3481 gccctaacaa gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc
     3541 agcagtgatc cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc
     3601 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     3661 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     3721 agagaggcct acgcagcaag tctcatcaag acgatctacc cgagtaacaa tctccaggag
     3781 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     3841 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     3901 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     3961 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     4021 cgccgtcaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     4081 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     4141 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     4201 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     4261 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     4321 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
     4381 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     4441 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     4501 ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac
     4561 aacaaacaac attacaatta catttacaat tatcgataca atgtgagacc gactggctgt
     4621 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     4681 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg
     4741 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     4801 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     4861 caggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     4921 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     4981 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     5041 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     5101 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     5161 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     5221 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     5281 acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt
     5341 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     5401 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     5461 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     5521 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     5581 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     5641 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     5701 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     5761 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     5821 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     5881 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     5941 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     6001 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aaggtctcat
     6061 tcgatgggcg gcaaattcat gcgtgaccac atggtccttc atgagtatgt aaatgctgct
     6121 gggattacag gtggctctgg aggtagagat catatggttc tccacgaata cgttaacgcc
     6181 gcaggcatca ctggcggtag tggaggacgc gaccatatgg tactacatga atatgtcaat
     6241 gcagccggaa taaccggata agcttagagc tttcgttcgt atcatcggtt tcgacaacgt
     6301 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt
     6361 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     6421 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     6481 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     6541 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     6601 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     6661 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     6721 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     6781 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     6841 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac
     6901 gctttacgag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa actatcagtg
     6961 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     7021 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc cagccgtgcg
     7081 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     7141 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     7201 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     7261 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     7321 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     7381 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     7441 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     7501 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     7561 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     7621 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     7681 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     7741 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     7801 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     7861 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     7921 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     7981 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     8041 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     8101 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     8161 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     8221 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     8281 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     8341 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     8401 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     8461 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt
     8521 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     8581 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     8641 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     8701 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     8761 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     8821 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     8881 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     8941 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     9001 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     9061 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     9121 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     9181 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     9241 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     9301 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     9361 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     9421 aaattgccct agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca
     9481 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     9541 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     9601 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     9661 tgtctggcca gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc
     9721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
     9841 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     9901 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     9961 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    10021 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    10081 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    10141 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
    10201 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
    10261 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
    10321 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
    10381 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    10441 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    10501 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    10561 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
    10621 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10681 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10741 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10921 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11401 tacggttcct gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta
    11461 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    11521 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
    11581 ttaatgtact ggggttgaac actct
//
