LOCUS       sequence-25501-e         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC249
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..48)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(70..90)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(154..174)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    250..297
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    322..445
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(470..475,476..493,494..500)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 ttctccttta ctcatttttt ctaccggtac cttacgcttc ttctttggag cagtcatttt
       61 ttctgccggt accttacgct tcttctttgg agcagtcatt ttttctgccg gtaccttacg
      121 cttcttcttt ggagcagtca ttttttctgc cggtacctta cgcttcttct ttggagcagt
      181 cattttttct gagctcctga aaatgttcta tgttatgtta gtatcattcg aaacataccc
      241 ggggatcttg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga
      301 gcggctagcc cagtgaatta tcaactatgt ataataaagt tgaacgagaa acgtaaaatg
      361 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
      421 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
      481 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
      541 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccat
//
