LOCUS       sequence-255010-        9255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Franklin B, Koob J, Kellner MJ, Ladha 
            A, Joung J, Kirchgatterer P, Cox DBT, Zhang F
  TITLE     A cytosine deaminase for programmable single-base RNA editing.
  JOURNAL   Science. 2019 Jul 11. pii: science.aax7063. doi: 
            10.1126/science.aax7063.
  PUBMED    31296651
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Jul 11. pii: science.aax7063. doi: 10.1126/science.aax7063."
COMMENT     Sequence Label: pC0096 RESCUE truncation (Cas13 delta 892-1095)
FEATURES             Location/Qualifiers
     source          1..9255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      683..692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4612..4629)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4618..4842
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4888..5316
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5185..5206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5325..5345)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5330..5659
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5510..5645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5572..5591
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5726..6520
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5780..5799)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6390..6409
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6694..6815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6731..6750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6785..6804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6864..6880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6864..6880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6877..6899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6888..6904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6912..6918,6919..6936,6937..6942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6957..6978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7095..7112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7266..7854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7346..7365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8025..8816,8817..8885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8648..8667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8886..8990)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9065..9084)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgccaccat ggagaagccc ctgctgccta acgtgtatac
      721 cctgaagcac aagttctttt ggggcgcctt cctgaatatc gcccggcaca acgcctttat
      781 caccatctgc cacatcaatg agcagctggg cctgaaaacc cccagcaacg acgataagat
      841 cgtggacgtg gtgtgcgaga catggaacaa tatcctgaac aatgaccacg atctgctgaa
      901 gaagtcccag ctgaccgagc tgatcctgaa gcacttccct tttctgacag ccatgtgcta
      961 ccacccccct aagaaggagg gcaagaagaa gggccaccag aaggagcagc agaaggagaa
     1021 ggagagcgag gcacagtccc aggcagaggc cctgaaccca tccaagctga tcgaggccct
     1081 ggagatcctg gtgaatcagc tgcactctct ggcgaactac tatagcgcct ataagcacaa
     1141 gaagcccgac gccgagaagg atatcttcaa gcacctgtat aaggccttcg acgcctctct
     1201 gagaatggtg aaggaggatt ataaggccca cttcaccgtg aatctgacaa gggactttgc
     1261 ccacctgaac cgcaagggca agaacaagca ggacaatccc gacttcaaca gatacagatt
     1321 cgagaaggat ggcttcttta ccgagtctgg cctgctgttc tttacaaatc tgtttctgga
     1381 caagagggat gcctattgga tgctgaagaa ggtgtccggc ttcaaggcct ctcacaagca
     1441 gcgcgagaag atgaccacag aggtgttttg caggagccgc atcctgctgc ccaagctgag
     1501 gctggagtcc cgctacgacc acaaccagat gctgctggat atgctgtctg agctgagcag
     1561 atgtcctaag ctgctgtatg agaagctgag cgaggagaat aagaagcact tccaggtgga
     1621 ggccgacggc tttctggatg agatcgagga ggagcagaac ccattcaagg acaccctgat
     1681 ccggcaccag gatagattcc cctactttgc cctgaggtat ctggacctga atgagtcctt
     1741 caagtctatc cgctttcagg tggatctggg cacataccac tattgtatct acgacaagaa
     1801 gatcggcgat gagcaggaga agaggcacct gacccgcaca ctgctgtcct tcggccggct
     1861 gcaggacttt accgagatca acagacccca ggagtggaag gccctgacca aggacctgga
     1921 ttacaaggag acatccaatc agcctttcat ctctaagacc acaccacact atcacatcac
     1981 cgacaacaag atcggctttc ggctgggcac aagcaaggag ctgtacccct ccctggagat
     2041 caaggatggc gccaatagaa tcgccaagta cccttataac tccggcttcg tggcccacgc
     2101 ctttatctct gtgcacgagc tgctgcctct gatgttctac cagcacctga ccggcaagag
     2161 cgaggacctg ctgaaggaga cagtgcggca catccagaga atctataagg acttcgagga
     2221 ggagcggatc aataccatcg aggatctgga gaaggcaaac cagggcagac tgccactggg
     2281 agcctttccc aagcagatgc tgggcctgct gcagaataag cagcctgatc tgtccgagaa
     2341 ggccaagatc aagatcgaga agctgatcgc cgagacaaag ctgctgtctc acaggctgaa
     2401 cacaaagctg aagagctccc caaagctggg caagcggaga gagaagctga tcaagacagg
     2461 cgtgctggcc gactggctgg tgaaggattt catgcgcttt cagcccgtgg cctacgacgc
     2521 ccagaatcag cctatcaagt ctagcaaggc caactccacc gagttctggt ttatcaggcg
     2581 cgccctggcc ctgtacggag gagagaagaa taggctggag ggctatttca agcagaccaa
     2641 cctgatcggc aacacaaatc cacacccctt cctgaacaag tttaattgga aggcctgcag
     2701 gaatctggtg gatttctacc agcagtatct ggagcagcgc gagaagtttc tggaggccat
     2761 caagaaccag ccatgggagc cctaccagta ttgcctgctg ctgaagatcc ctaaggagaa
     2821 caggaagaat ctggtgaagg gatgggagca gggaggaatc tctctgccac ggggcctgtt
     2881 taccgaggcc atcagagaga cactgtctga ggacctgatg ctgagcaagc caatccgcaa
     2941 ggagatcaag aagcacggcc gggtgggctt catcagcaga gccatcaccc tgtactttaa
     3001 ggagaagtat caggataagc accagagctt ctacaatctg tcctataagc tggaggcaaa
     3061 ggcaccactg ctgaagcggg aggagcacta cgagtattgg cagcagaaca agcctcagtc
     3121 tccaaccgag agccagaggc tggagctgca cacaagcgac cgctggaagg attacctgct
     3181 gtataagaga tggcagcacc tggagaagaa gctgcggctg taccggaatc aggacgtgat
     3241 gctgtggctg atgaccctgg agctgacaaa gaaccacttc aaggagctga acctgaatta
     3301 tcaccagctg aagctggaga atctggccgt gaacgtgcag gaggccgatg ccaagctgaa
     3361 ccctggatcc agcctccaaa aaaagttgga ggaattagaa ctaggatccc agctgcattt
     3421 accgcaggtt ttagctgacg ctgtctcacg cctggtcata ggtaagtttg gtgacctgac
     3481 cgacaacttc tcctcccctc acgctcgcag aataggtctg gctggagtcg tcatgacaac
     3541 aggcacagat gttaaagatg ccaaggtgat atgtgtttct acaggatcta aatgtattaa
     3601 tggtgaatac ctaagtgatc gtggccttgc attaaatgac tgccatgcag aaatagtatc
     3661 tcggagatcc ttgctcagat ttctttatac acaacttgag ctttacttaa ataacgagga
     3721 tgatcaaaaa agatccatct ttcagaaatc agagcgaggg gggtttaggc tgaaggagaa
     3781 tatacagttt catctgtaca tcagcacctc tccctgtgga gatgccagaa tcttctcacc
     3841 acatgaggca atcctggaag aaccagcaga tagacaccca aatcgtaaag caagaggaca
     3901 gctacggacc aaaatagagg ctggtcaggg gacgattcca gtgcgcaaca atgcgagcat
     3961 ccaaacgtgg gacggggtgc tgcaagggga gcggctgctc accatgtcct gcagtgacaa
     4021 gattgcacgc tggaacgtgg tgggcatcca gggatcactg ctcagcattt tcgtggagcc
     4081 catttacttc tcgagcatca tcctgggcag cctttaccac ggggaccacc tttccagggc
     4141 catgtaccag cggatctcca acatagagga cctgccacct ctctacaccc tcaacaagcc
     4201 tttgctcaca ggcatcagca atgcagaagc acggcagcca gggaaggccc ccatattcag
     4261 tgtcaactgg acggtaggcg actccgctat tgaggtcatc aacgccacga ctgggaaggg
     4321 agagctgggc cgcgcgtccc gcctgtgtaa gcacgcgttg tactgtcgct ggatgcgtgt
     4381 gcacggcaag gttccctccc acttactacg ctccaagatt accaagccca acgtgtacca
     4441 tgagacaaag ctggcggcaa aggagtacca ggccgccaag gcgcgtctgt tcacagcctt
     4501 catcaaggcg gggctggggg cctgggtgga gaagcccacc gagcaggacc agttctcact
     4561 cacgtaagcg gccgctcgag tctagagggc ccatttaaac ccgctgatca gcctcgactg
     4621 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4681 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4741 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4801 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4861 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5101 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5221 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5281 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5341 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5401 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5461 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5521 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5581 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     5641 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     5701 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     5761 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5821 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5881 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5941 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6001 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6061 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6121 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6181 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6241 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6301 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6361 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6421 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6481 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6541 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6601 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6661 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6781 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6841 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6901 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6961 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7021 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9001 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9061 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9121 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     9181 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     9241 ccagatatac gcgtt
//
