LOCUS       sequence-255019-        9443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Kmiecik TE, Shalloway D
  TITLE     Activation and suppression of pp60c-src transforming ability by 
            mutation of its primary sites of tyrosine phosphorylation.
  JOURNAL   Cell. 1987 Apr 10. 49(1):65-73.
  PUBMED    3103925
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1987 
            Apr 10. 49(1):65-73."
COMMENT     Sequence Label: pcSrc527
FEATURES             Location/Qualifiers
     source          1..9443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     complement(981..1000)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             2401..2442
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     rep_origin      complement(5990..6578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6070..6089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6749..7540,7541..7609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7372..7391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7610..7714)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7782..7800
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcaggcgca
      901 gaactggtag gtatggaaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      961 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     1021 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     1081 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     1141 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaca
     1201 gcacacaagg tagtttcacc aaaccaaaag cgctaccacc cgcaattttc ctcatttgtg
     1261 gggatcgcgc atggcaagga attcccagtc gtccggtagg gggcccctgc tatttaggca
     1321 agcttaccat gttagcacct aagcatacag atattctcaa ggtgcttgtc aattcatcgc
     1381 ggacaggtat aagacgtaaa cgaagcacct cacacctgga tgatacatgc tcagatgaag
     1441 tgcagctttg gggtcctaca gcaagaatct ttgcatctat cctagccccg ggggtagcag
     1501 ctgcgcaagc cttaagagaa attgagagac tagcctgttg gtccgttaaa caggctaact
     1561 tgacaacatc actcctcggg gacttattgg atgatgtcac gagtattcga cacgcggtcc
     1621 tgcagaaccg agcggctatt gacttcttgc tcctagctca cggccatggc tgtgaggacg
     1681 ttgccggaat gtgctgtttc aatttgagtg atcacagtga gtctatacag aagaagttcc
     1741 agctaatgaa ggaacatgtc aataagatcg gcgtggacag cgacccaatt ggaagttggc
     1801 tgcgaggact attcggggga ataggagaat gggccgttca tttgctgaaa ggactgcttt
     1861 tggggcttgt agttattttg ttgctagtag tgtgcctgcc ttgccttttg caaatcgtat
     1921 gcggtaacat cagaaagatg attaataact ccatcagcta ccacacggaa tataagaagc
     1981 tgcaaaaggc ctgtgggcag cctgaaagca gaatagtata aggcagtaca tgggtggtgg
     2041 tatagcgctt gcgagtcggg ttgtaacggg acatggctta actaagggga ctatggcatg
     2101 tataggcgca aagcggggtt tcggttgtac gcgattagga gtccccttag gatatagtag
     2161 acacgctttt gcatatgata cataacttcc ctgttttgcc cttagactat tcaagttgcc
     2221 tctgtggatt agggctggag gcagctcgga tggtctgacg gccagataag gcaggaaaga
     2281 cagctattgg taattgtgaa atacgctttt gtctgtgtgc tgcaggagct gagctgactc
     2341 tgctggtggc ctcgcgtacc actgtggcca ggcggtagct gggacgtgca gccgaccacc
     2401 atggggagca gcaagagcaa gcccaaggac cccagccagc gccggcgcag cctggagcca
     2461 cccgacagca cccaccacgg gggattccca gcctcgcaga cccccaacaa gacagcagcc
     2521 cccgacacgc accgcacccc cagccgctcc tttgggaccg tggccaccga gcccaagctc
     2581 ttcgggggct tcaacacttc tgacaccgtc acgtcgccgc agcgtgccgg ggcactggct
     2641 ggcggcgtca ccactttcgt ggctctctat gactacgagt cccggactga aacggacttg
     2701 tccttcaaga aaggagaacg cctgcagatt gtcaacaaca cggaaggtga ctggtggctg
     2761 gctcattccc tcactacagg acagacgggc tacatcccca gtaactatgt cgcgccctca
     2821 gactccatcc aggctgaaga gtggtacttt gggaagatca ctcgtcggga gtccgagcgg
     2881 ctgctgctca accccgaaaa cccccgggga accttcttgg tccgggagag cgagacgaca
     2941 aaaggtgcct attgcctctc cgtttctgac tttgacaacg ccaaggggct caatgtgaag
     3001 cactacaaga tccgcaagct ggacagcggc ggcttctaca tcacctcacg cacacagttc
     3061 agcagcctgc agcagctggt ggcctactac tccaaacatg ctgatggctt gtgccaccgc
     3121 ctgaccaacg tctgccccac gtccaagccc cagacccagg gactcgccaa ggacgcgtgg
     3181 gaaatccccc gggagtcgct gcggctggag gtgaagctgg ggcagggctg ctttggagag
     3241 gtctggatgg ggacctggaa cggcaccacc agagtggcca taaagactct gaagcccggc
     3301 accatgtccc cggaggcctt cctgcaggaa gcccaagtga tgaagaagct ccggcatgag
     3361 aagctggttc agctgtacgc agtggtgtcg gaagagccca tctacatcgt cactgagtac
     3421 atgagcaagg ggagcctcct ggatttcctg aagggagaga tgggcaagta cctgcggctg
     3481 ccacagctcg tcgatatggc tgctcagatt gcatccggca tggcctatgt ggagaggatg
     3541 aactacgtgc accgagacct gcgggcggcc aacatcctgg tgggggagaa cctggtgtgc
     3601 aaggtggctg actttgggct ggcacgcctc atcgaggaca acgagtacac agcacggcaa
     3661 ggtgccaagt tccccatcaa gtggacagcc cccgaggcag ccctctatgg ccggttcacc
     3721 atcaagtcgg atgtctggtc cttcggcatc ctgctgactg agctgaccac caagggccgg
     3781 gtgccatacc caggtgagag ttagcatcaa ccccacaccc cgtgggttgg gcacagcgtg
     3841 tgacccggcc ccgctgccct tctttccaca gggatggtca acagggaggt gctggaccag
     3901 gtggagaggg gctaccgcat gccctgcccg cccgagtgcc ccgagtcgct gcatgacctc
     3961 atgtgccagt gctggcggaa ggaccctgag gagcggccca cttttgagta cctgcaggcc
     4021 ttcctggagg actacttcac ctcgacagag ccccagttcc agcctggaga gaacctatag
     4081 atctctagaa gcttatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     4141 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
     4201 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     4261 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     4321 agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
     4381 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     4441 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     4501 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     4561 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
     4621 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     4681 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     4741 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     4801 acattaaatg gccatagtac ttaaagttac attggcttcc ttgaaataaa catggagtat
     4861 tcagaatgtg tcataaatat ttctaatttt aagatagtat ctccattggc tttctacttt
     4921 ttcttttatt tttttttgtc ctctgtcttc catttgttgt tgttgttgtt tgtttgtttg
     4981 tttgttggtt ggttggttaa ttttttttta aagatcctac actatagttc aagctagact
     5041 attagctact ctgtaaccca gggtgacctt gaagtcatgg gtagcctgct gttttagcct
     5101 tcccacatct aagattacag gtatgagcta tcatttttgg tatattgatt gattgattga
     5161 ttgatgtgtg tgtgtgtgat tgtgtttgtg tgtgtgattg tgtatatgtg tgtatggttg
     5221 tgtgtgattg tgtgtatgta tgtttgtgtg tgattgtgtg tgtgtgattg tgcatgtgtg
     5281 tgtgtgtgat tgtgtttatg tgtatgattg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     5341 tgtgtgtgtg tgtgttgtgt atatatattt atggtagtga gaggcaacgc tccggctcag
     5401 gtgtcaggtt ggtttttgag acagagtctt tcacttagct tggaattctc atgtttgaca
     5461 gcttatcatc gatgataagc tgtcaaacat gagaatttcg accgatgccc ttgagagcct
     5521 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     5581 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     5641 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     5701 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     5761 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     5821 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     5881 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6421 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6661 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6721 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6781 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6841 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6901 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6961 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7021 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     7081 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7141 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7201 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7261 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7321 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     7381 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7441 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7501 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7561 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7621 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7681 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7741 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7801 cctttcgtct tcaagaattc attcttttat tgagtccaga ttagaaaaag aagagaattt
     7861 atgaacggac cttttgacag atttcttttt gaaaatactc attcctcttc agatttgttt
     7921 taaatgaagt tttctttctt taacttgttt gcacgcagct cagctgtgtt ctttcttaaa
     7981 tgaggtctgg taaagaaaag gccctaccaa ggtcttagct gtacttgtga catgatctct
     8041 ctattaggtt aaagtttttt tctgttatgt gatgctcata aaactgagtt ataacaatat
     8101 taataataac ttcactaatt gattctgtga gggatgagaa gagggaggaa taggatgaga
     8161 aaaacagagg gaactatttg ccttgtattt gacatactta gaagataagg gaaacatgtt
     8221 tggaatgtca gttccccagg ctactttatg tcaatgttag aaatggaaac aatgattttc
     8281 attggctaca aacaggagag gaggctgtgg tgattttggg gtatgcgttg tgttcattgt
     8341 gattctataa gcaccccatc ttccaaagag agctcaacaa tagtcaggaa gcctcgccct
     8401 aaagcccatc ctcccccccg ccccaccccc atgactttgt ttccttgtca gactttacct
     8461 gagaggaaat gaaattaata agctgtgaaa aagtatccca tgtgggctgc tcttaagtct
     8521 cccgttgcct tttcctcctg tgttctgcgt tctagctgtt tgcgagtagg tgaggctggt
     8581 gactcactgc cttcaagcag cagcaggact ggtgagtcat agctttcagg ctggggttgt
     8641 ggttcactcc catggacggt ttaacccgtg gttgtctctt agggggtcag tgggaacatc
     8701 cttcgtatgg tcgtgatctt cttaaatcct ctccccagga ccaggcaata acagtgtcct
     8761 ggcaaaagct ccagtactcc atcttctcag gacatcccag tccacatgac taatctttcc
     8821 caccttctac atttgagcgt ttcctgcctt cacattatag aaactatttt gggaagtatt
     8881 atttttaaac atgacttttc ccttaattct accataataa gtgctgggtt aagctttgct
     8941 cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac ttgttctatg
     9001 ccctaggggg cggggggaag ctaagccagc tttttttaac atttaaaatg ttaattccat
     9061 tttaaatgca cagatgtttt tatttcataa gggtttcaat gtgcatgaat gctgcaatat
     9121 tcctgttacc aaagctagta taaataaaaa tagataaacg tggaaattac ttagagtttc
     9181 tgtcattaac gtttccttcc tcagttgaca acataaatgc gctgctgaga agccagtttg
     9241 catctgtcag gatcaatttc ccattatgcc agtcatatta attactagtc aattagttga
     9301 tttttatttt tgacatatac atgtgaaaga ccccacctgt aggtttggca agctagctta
     9361 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
     9421 aaggtcagga acagatggaa cag
//
