LOCUS       sequence-255027-        8368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of the
            blue fluorescent protein AmCyan and the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8368)
  TITLE     pUltra series
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Smurf
FEATURES             Location/Qualifiers
     source          1..8368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2004..2037
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        2069..3280
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3117..3136
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3312..3998
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     CDS             4017..4073
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4095..4148
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    4170..4203
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    4215..4803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4268..4288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4686..4697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4894..5127
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5199..5320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5236..5255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5290..5309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5360..5495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5515..5534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5516..5534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5544..5561)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5544..5560)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5553..5575)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5702..6157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5789..5808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5999..6020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6183..6287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6288..6356,6357..7148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6506..6525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7319..7907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7808..7827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8061..8078
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8195..8216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8231..8236,8237..8254,8255..8261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8269..8285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8274..8296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8293..8309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8293..8309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8328..8348
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8330..8348
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttaatta acgataactt cgtataatgt gtactatacg aagttatctc
     2041 gagattaacc cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg
     2101 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     2161 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     2221 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     2281 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     2341 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     2401 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     2461 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     2521 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     2581 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     2641 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     2701 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     2761 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     2821 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     2881 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     2941 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     3001 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     3061 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     3121 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     3181 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     3241 gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggg ctgcaggtcc
     3301 gatccaccgg tatggccctg tccaacaagt tcatcggcga cgacatgaag atgacctacc
     3361 acatggacgg ctgcgtgaac ggccactact tcaccgtgaa gggcgagggc agcggcaagc
     3421 cctacgaggg cacccagacc tccaccttca aggtgaccat ggccaacggc ggccccctgg
     3481 ccttctcctt cgacatcctg tccaccgtgt tcatgtacgg caaccgctgc ttcaccgcct
     3541 accccaccag catgcccgac tacttcaagc aggccttccc cgacggcatg tcctacgaga
     3601 gaaccttcac ctacgaggac ggcggcgtgg ccaccgccag ctgggagatc agcctgaagg
     3661 gcaactgctt cgagcacaag tccaccttcc acggcgtgaa cttccccgcc gacggccccg
     3721 tgatggccaa gaagaccacc ggctgggacc cctccttcga gaagatgacc gtgtgcgacg
     3781 gcatcttgaa gggcgacgtg accgccttcc tgatgctgca gggcggcggc aactacagat
     3841 gccagttcca cacctcctac aagaccaaga agcccgtgac catgcccccc aaccacgtgg
     3901 tggagcaccg catcgccaga accgacctgg acaagggcgg caacagcgtg cagctgaccg
     3961 agcacgccgt ggcccacatc acctccgtgg tgcccttcgt gtacaaggga tctggagcaa
     4021 caaacttctc actactcaaa caagcaggtg acgtggagga gaatcccggg ccttctagag
     4081 gatccggctc cggcgagggc aggggaagtc tactaacatg cggggacgtg gaggaaaatc
     4141 ccggcccagc tagcgtgtga tcagaattca taacttcgta taatgtgtac tatacgaagt
     4201 tatgtcgact cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4261 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4321 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4381 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4441 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4501 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4561 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4621 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4681 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4741 ctcttccgca tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4801 cgcctggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     4861 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctacgtaac tcccaacgaa
     4921 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5101 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5161 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5461 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5521 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5581 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5641 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5701 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5761 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5821 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5881 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5941 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6001 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6061 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6121 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6181 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6241 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6301 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6361 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6421 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6481 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6541 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6601 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6661 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6721 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6781 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6841 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6901 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6961 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7021 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7081 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7141 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7201 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7261 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7321 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7381 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7441 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7501 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7561 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7621 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7681 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7741 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7801 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctcacttg
     7861 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7921 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7981 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8041 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8101 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8281 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8341 actaaaggga acaaaagctg gagctgca
//
