LOCUS       sequence-25503-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC31
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(149..454)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(251..270)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aagctgacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
       61 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
      121 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
      181 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
      241 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
      301 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
      361 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
      421 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
      481 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
      541 ttccagcgtt cggcacg
//
