LOCUS       sequence-255033-       11372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 C-terminal gateway cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11372)
  TITLE     Gateway compatible mammalian Cas9 vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dCas9 gateway compatible
FEATURES             Location/Qualifiers
     source          1..11372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     669..689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     715..739
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      827..836
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(833..859,860..862,863..3346,3347..3352,3353..3418,
                     3419..3421,3422..4936)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4949..4969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5006..5130
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5155..5160,5161..5178,5179..5185)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5239..5898
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5305..5324)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6240..6545
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6424..6443
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6586..6710)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6843..6862)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6887..6935
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7137..7565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7224..7243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7434..7455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7574..7594)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7579..7908
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7759..7894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7821..7840
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7975..8769
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8029..8048)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8639..8658
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8945..9066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8982..9001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9036..9055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9115..9131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9115..9131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9128..9150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9139..9155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9163..9169,9170..9187,9188..9193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9208..9229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9346..9363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9517..10105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9597..9616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10276..11067,11068..11136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10899..10918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11137..11241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11316..11335)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
       61 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
      121 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      661 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      721 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      781 acaccgggac cgatccagcc tccggactct agaggatcga acccttgcca ccatggacaa
      841 gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg tcattacgga
      901 cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat
      961 aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg
     1021 gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca
     1081 ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga
     1141 gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt
     1201 ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt
     1261 agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa
     1321 atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa
     1381 actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc
     1441 atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga
     1501 aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc
     1561 cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa
     1621 gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg
     1681 cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag
     1741 tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa
     1801 gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact
     1861 gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat
     1921 tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat
     1981 ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg
     2041 cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct
     2101 caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat
     2161 cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc
     2221 gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga
     2281 taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc
     2341 taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga
     2401 gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga
     2461 gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca
     2521 gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt
     2581 ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga
     2641 caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct
     2701 tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt
     2761 cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc
     2821 aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct
     2881 taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg actctctcac
     2941 ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca
     3001 catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt
     3061 cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat
     3121 ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag
     3181 gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa
     3241 cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta
     3301 cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggctg ctatcgtgcc
     3361 ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaagc
     3421 tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg
     3481 gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc
     3541 tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga
     3601 gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta
     3661 cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt
     3721 ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca
     3781 tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa
     3841 gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc
     3901 aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat
     3961 gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat
     4021 cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt
     4081 ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg
     4141 aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa
     4201 aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt
     4261 actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact
     4321 gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga
     4381 ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct
     4441 ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg
     4501 taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga
     4561 aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa
     4621 acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc
     4681 cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag
     4741 ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc
     4801 cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct
     4861 ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc
     4921 tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgt cgccagggat
     4981 ccgtcgactt gacgcgttga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta
     5041 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     5101 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     5161 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     5221 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     5281 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     5341 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     5401 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     5461 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     5521 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     5581 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     5641 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     5701 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     5761 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     5821 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     5881 tggcagggcg gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg
     5941 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     6001 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     6061 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     6121 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     6181 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     6241 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     6301 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     6361 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     6421 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     6481 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     6541 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     6601 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     6661 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatcatc
     6721 tagagcggcc gcaggtacct gaataactaa ggccgcttcc ctttagtgag ggttaatgct
     6781 tcgagcagac aaagggttcg atccctaccg gttagtaatg agtttaaacg ggggaggcta
     6841 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     6901 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6961 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     7021 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     7081 ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt atccccacgc
     7141 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7201 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7261 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7321 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7381 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7441 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7501 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7561 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7621 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7681 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7741 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7801 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7861 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7921 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     7981 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     8041 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     8101 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     8161 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     8221 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8281 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8341 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8401 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8461 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8521 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8581 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8641 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8701 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8761 ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc
     8821 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8941 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9061 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11281 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    11341 ttaagccagt atctgctccc tgcttgtgtg tt
//
