LOCUS       sequence-25504-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC48
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(132..137,138..155,156..162)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(282..301)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
       61 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
      121 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
      181 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      241 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      301 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
      361 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
      421 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
      481 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
      541 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
//
