LOCUS       sequence-255041-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human cGAS with R71/75E and C396/397A point mutations; 
            Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS R71/75E C396/7A-HA
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3870..3896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3906..4494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3959..3979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4377..4388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4508..5007
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4543..4566)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4940..4959
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5028..5627
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5028..5047)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5524..5544
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5765..5998
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6070..6191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6107..6126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6161..6180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6231..6366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6386..6405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6387..6405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6415..6432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6415..6431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6424..6446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6573..7028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6660..6679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6870..6891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7054..7158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7159..7227,7228..8019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7377..7396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8190..8778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8679..8698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8932..8949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9066..9087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9102..9107,9108..9125,9126..9132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9140..9156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9145..9167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9164..9180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9164..9180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9199..9219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9201..9219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9247..9473
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagagcc accatgcagc cttggcacgg aaaggccatg cagagagctt
     2341 ccgaggccgg agccactgcc cccaaggctt ccgcacggaa tgccaggggc gccccgatgg
     2401 atcccaccga gtctccggct gcccccgagg ccgccctgcc taaggcggga aagttcggcc
     2461 ccgccaggaa gtcgggatcc cggcagaaaa agagcgcccc ggacacccag gaggagccgc
     2521 ccgtcgaggc aactggggcc cgcgccaaaa aggcccctca gcgcgcccag gacacgcagc
     2581 cgtctgacgc caccagcgcc cctggggcag aggggctgga gcctcctgcg gctcgggagc
     2641 cggctctttc cagggctggt tcttgccgcc agaggggcgc gcgctgctcc acgaagccaa
     2701 gacctccgcc cgggccctgg gacgtgccca gccccggcct gccggtctcg gcccccattc
     2761 tcgtacggag ggatgcggcg cctggggcct cgaagctccg ggcggttttg gagaagttga
     2821 agctcagccg cgatgatatc tccacggcgg cggggatggt gaaaggggtt gtggaccacc
     2881 tgctgctcag actgaagtgc gactccgcgt tcagaggcgt cgggctgctg aacaccggga
     2941 gctactatga gcacgtgaag atttctgcac ctaatgaatt tgatgtcatg tttaaactgg
     3001 aagtccccag aattcaacta gaagaatatt ccaacactcg tgcatattac tttgtgaaat
     3061 ttaaaagaaa tccgaaagaa aatcctctga gtcagttttt agaaggtgaa atattatcag
     3121 cttctaagat gctgtcaaag tttaggaaaa tcattaagga agaaattaac gacattaaag
     3181 atacagatgt catcatgaag aggaaaagag gagggagccc tgctgtaaca cttcttatta
     3241 gtgaaaaaat atctgtggat ataaccctgg ctttggaatc aaaaagtagc tggcctgcta
     3301 gcacccaaga aggcctgcgc attcaaaact ggctttcagc aaaagttagg aagcaactac
     3361 gactaaagcc attttacctt gtacccaagc atgcaaagga aggaaatggt ttccaagaag
     3421 aaacatggcg gctatccttc tctcacatcg aaaaggaaat tttgaacaat catggaaaat
     3481 ctaaaacggc cgctgaaaac aaagaagaga aatgttgcag gaaagattgt ttaaaactaa
     3541 tgaaatacct tttagaacag ctgaaagaaa ggtttaaaga caaaaaacat ctggataaat
     3601 tctcttctta tcatgtgaaa actgccttct ttcacgtatg tacccagaac cctcaagaca
     3661 gtcagtggga ccgcaaagac ctgggcctct gctttgataa ctgcgtgaca tactttcttc
     3721 agtgcctcag gacagaaaaa cttgagaatt attttattcc tgaattcaat ctattctcta
     3781 gcaacttaat tgacaaaaga agtaaagaat ttctgacaaa gcaaattgaa tatgaaagaa
     3841 acaatgagtt tccagttttt gatgaatttt acccatacga tgttccagat tacgcttgag
     3901 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     4501 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     4561 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     4621 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     4681 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     4741 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     4801 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     4861 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     4921 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     4981 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     5041 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5101 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5161 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5221 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5281 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5341 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5401 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5461 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5521 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5581 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     5641 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct
     5701 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5761 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5821 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5881 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5941 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6001 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6061 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6181 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6241 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6301 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6361 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6541 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6601 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6661 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6721 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6781 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6841 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6901 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6961 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7021 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7561 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7981 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8041 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8941 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9001 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9061 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9181 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     9241 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     9301 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     9361 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     9421 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aac
//
