LOCUS       sequence-255048-        9251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human cGAS-Delta-N (aa160-522)-HA with an N terminal 
            fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-TIRAP-cGAS-Delta-N-HA
FEATURES             Location/Qualifiers
     source          1..9251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2525..2534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3875..3901
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3911..4499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3964..3984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4382..4393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4513..5012
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4548..4571)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4945..4964
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5033..5632
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5033..5052)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5529..5549
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5770..6003
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6075..6196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6112..6131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6166..6185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6236..6371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6391..6410)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6392..6410)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6420..6437)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6420..6436)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6429..6451)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6578..7033
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6665..6684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6875..6896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7059..7163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7164..7232,7233..8024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7382..7401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8195..8783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8684..8703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8937..8954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9071..9092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9107..9112,9113..9130,9131..9137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9145..9161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9150..9172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9169..9185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9169..9185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9204..9224
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9206..9224
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagagccacc atggcatcat cgacctccct cccagctcct ggctctcggc ctaagaagcc
     2581 tctaggcaag atggctgact ggttcaggca gaccctgctg aagaagccca agaagaggcc
     2641 caactcccca gaaagcacct ccagcgatgc ttcacagcct acctcacagg acagcccact
     2701 acccccaagc ctcagctcag tcacgtctcc cagcctgcca cccacacatg cgagtgacag
     2761 tggcagtagt cgctggagca aagaccctgg ggcctcgaag ctccgggcgg ttttggagaa
     2821 gttgaagctc agccgcgatg atatctccac ggcggcgggg atggtgaaag gggttgtgga
     2881 ccacctgctg ctcagactga agtgcgactc cgcgttcaga ggcgtcgggc tgctgaacac
     2941 cgggagctac tatgagcacg tgaagatttc tgcacctaat gaatttgatg tcatgtttaa
     3001 actggaagtc cccagaattc aactagaaga atattccaac actcgtgcat attactttgt
     3061 gaaatttaaa agaaatccga aagaaaatcc tctgagtcag tttttagaag gtgaaatatt
     3121 atcagcttct aagatgctgt caaagtttag gaaaatcatt aaggaagaaa ttaacgacat
     3181 taaagataca gatgtcatca tgaagaggaa aagaggaggg agccctgctg taacacttct
     3241 tattagtgaa aaaatatctg tggatataac cctggctttg gaatcaaaaa gtagctggcc
     3301 tgctagcacc caagaaggcc tgcgcattca aaactggctt tcagcaaaag ttaggaagca
     3361 actacgacta aagccatttt accttgtacc caagcatgca aaggaaggaa atggtttcca
     3421 agaagaaaca tggcggctat ccttctctca catcgaaaag gaaattttga acaatcatgg
     3481 aaaatctaaa acgtgctgtg aaaacaaaga agagaaatgt tgcaggaaag attgtttaaa
     3541 actaatgaaa taccttttag aacagctgaa agaaaggttt aaagacaaaa aacatctgga
     3601 taaattctct tcttatcatg tgaaaactgc cttctttcac gtatgtaccc agaaccctca
     3661 agacagtcag tgggaccgca aagacctggg cctctgcttt gataactgcg tgacatactt
     3721 tcttcagtgc ctcaggacag aaaaacttga gaattatttt attcctgaat tcaatctatt
     3781 ctctagcaac ttaattgaca aaagaagtaa agaatttctg acaaagcaaa ttgaatatga
     3841 aagaaacaat gagtttccag tttttgatga attttaccca tacgatgttc cagattacgc
     3901 ttgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3961 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4021 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4081 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4141 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4201 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4261 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4321 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4381 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4441 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4501 tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     4561 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4621 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     4681 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     4741 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     4801 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     4861 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     4921 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     4981 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     5041 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5101 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5161 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5221 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5281 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5341 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5401 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5461 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5521 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5581 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     5641 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
     5701 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5761 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     5821 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5881 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5941 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6001 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6061 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6181 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6241 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6301 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6361 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6421 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6481 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6541 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7021 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7081 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7141 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7201 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7261 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7321 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7381 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7441 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7501 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7561 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7621 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7681 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7741 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7801 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7861 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7921 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8881 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8941 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9001 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9061 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9181 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9241 tgcaagctta a
//
