LOCUS       sequence-255049-        9461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human cGAS-HA with R71/75E point mutation; Puromycin 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS R71/75E-HA
FEATURES             Location/Qualifiers
     source          1..9461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3870..3896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3906..4494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3959..3979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4377..4388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4508..4995
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4543..4566)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4928..4947
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5016..5615
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5016..5035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5512..5532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5753..5986
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6058..6179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6095..6114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6149..6168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6219..6354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6374..6393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6375..6393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6403..6420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6403..6419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6412..6434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6561..7016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6648..6667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6858..6879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7042..7146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7147..7215,7216..8007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7365..7384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8178..8766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8667..8686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8920..8937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9054..9075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9090..9095,9096..9113,9114..9120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9128..9144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9133..9155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9152..9168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9152..9168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9187..9207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9189..9207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9235..9461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagagcc accatgcagc cttggcacgg aaaggccatg cagagagctt
     2341 ccgaggccgg agccactgcc cccaaggctt ccgcacggaa tgccaggggc gccccgatgg
     2401 atcccaccga gtctccggct gcccccgagg ccgccctgcc taaggcggga aagttcggcc
     2461 ccgccaggaa gtcgggatcc cggcagaaaa agagcgcccc ggacacccag gaggagccgc
     2521 ccgtcgaggc aactggggcc cgcgccaaaa aggcccctca gcgcgcccag gacacgcagc
     2581 cgtctgacgc caccagcgcc cctggggcag aggggctgga gcctcctgcg gctcgggagc
     2641 cggctctttc cagggctggt tcttgccgcc agaggggcgc gcgctgctcc acgaagccaa
     2701 gacctccgcc cgggccctgg gacgtgccca gccccggcct gccggtctcg gcccccattc
     2761 tcgtacggag ggatgcggcg cctggggcct cgaagctccg ggcggttttg gagaagttga
     2821 agctcagccg cgatgatatc tccacggcgg cggggatggt gaaaggggtt gtggaccacc
     2881 tgctgctcag actgaagtgc gactccgcgt tcagaggcgt cgggctgctg aacaccggga
     2941 gctactatga gcacgtgaag atttctgcac ctaatgaatt tgatgtcatg tttaaactgg
     3001 aagtccccag aattcaacta gaagaatatt ccaacactcg tgcatattac tttgtgaaat
     3061 ttaaaagaaa tccgaaagaa aatcctctga gtcagttttt agaaggtgaa atattatcag
     3121 cttctaagat gctgtcaaag tttaggaaaa tcattaagga agaaattaac gacattaaag
     3181 atacagatgt catcatgaag aggaaaagag gagggagccc tgctgtaaca cttcttatta
     3241 gtgaaaaaat atctgtggat ataaccctgg ctttggaatc aaaaagtagc tggcctgcta
     3301 gcacccaaga aggcctgcgc attcaaaact ggctttcagc aaaagttagg aagcaactac
     3361 gactaaagcc attttacctt gtacccaagc atgcaaagga aggaaatggt ttccaagaag
     3421 aaacatggcg gctatccttc tctcacatcg aaaaggaaat tttgaacaat catggaaaat
     3481 ctaaaacgtg ctgtgaaaac aaagaagaga aatgttgcag gaaagattgt ttaaaactaa
     3541 tgaaatacct tttagaacag ctgaaagaaa ggtttaaaga caaaaaacat ctggataaat
     3601 tctcttctta tcatgtgaaa actgccttct ttcacgtatg tacccagaac cctcaagaca
     3661 gtcagtggga ccgcaaagac ctgggcctct gctttgataa ctgcgtgaca tactttcttc
     3721 agtgcctcag gacagaaaaa cttgagaatt attttattcc tgaattcaat ctattctcta
     3781 gcaacttaat tgacaaaaga agtaaagaat ttctgacaaa gcaaattgaa tatgaaagaa
     3841 acaatgagtt tccagttttt gatgaatttt acccatacga tgttccagat tacgcttgag
     3901 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     4501 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     4561 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     4621 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     4681 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     4741 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     4801 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     4861 ggaaggggtg ggtccggggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     4921 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     4981 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     5041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     5101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     5161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5221 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     5461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     5581 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     5641 cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg
     5701 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5761 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5821 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5881 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5941 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6001 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     6061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6181 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6241 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6301 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     6361 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6421 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6481 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6541 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6901 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6961 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     7021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7141 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7261 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7321 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7381 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7441 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7501 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7561 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7681 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7741 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7801 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7861 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7981 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8281 ccgaaggtaa ctggcttcag caaagcgcag ataccaaata ctgttcttct agtgtagccg
     8341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8881 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8941 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9001 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9061 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9121 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9181 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     9241 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     9301 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     9361 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     9421 gcagagatat tgtatttaag tgcctagctc gatacataaa c
//
