LOCUS       sequence-255053-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human cGAS-HA; Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS-HA
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4097..4123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4133..4721
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4186..4206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4604..4615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4735..5234
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4770..4793)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5167..5186
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5255..5854
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5255..5274)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5751..5771
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5992..6225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6297..6418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6334..6353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6388..6407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6458..6593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6613..6632)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6614..6632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6642..6659)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6642..6658)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6651..6673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6800..7255
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6887..6906)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7097..7118
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7281..7385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7386..7454,7455..8246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7604..7623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8417..9005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8906..8925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9159..9176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9293..9314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9329..9334,9335..9352,9353..9359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9367..9383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9372..9394
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9391..9407
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9391..9407
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9426..9446
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9428..9446
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagagccacc atgcagcctt ggcacggaaa ggccatgcag agagcttccg aggccggagc
     2581 cactgccccc aaggcttccg cacggaatgc caggggcgcc ccgatggatc ccaccgagtc
     2641 tccggctgcc cccgaggccg ccctgcctaa ggcgggaaag ttcggccccg ccaggaagtc
     2701 gggatcccgg cagaaaaaga gcgccccgga cacccaggag aggccgcccg tccgcgcaac
     2761 tggggcccgc gccaaaaagg cccctcagcg cgcccaggac acgcagccgt ctgacgccac
     2821 cagcgcccct ggggcagagg ggctggagcc tcctgcggct cgggagccgg ctctttccag
     2881 ggctggttct tgccgccaga ggggcgcgcg ctgctccacg aagccaagac ctccgcccgg
     2941 gccctgggac gtgcccagcc ccggcctgcc ggtctcggcc cccattctcg tacggaggga
     3001 tgcggcgcct ggggcctcga agctccgggc ggttttggag aagttgaagc tcagccgcga
     3061 tgatatctcc acggcggcgg ggatggtgaa aggggttgtg gaccacctgc tgctcagact
     3121 gaagtgcgac tccgcgttca gaggcgtcgg gctgctgaac accgggagct actatgagca
     3181 cgtgaagatt tctgcaccta atgaatttga tgtcatgttt aaactggaag tccccagaat
     3241 tcaactagaa gaatattcca acactcgtgc atattacttt gtgaaattta aaagaaatcc
     3301 gaaagaaaat cctctgagtc agtttttaga aggtgaaata ttatcagctt ctaagatgct
     3361 gtcaaagttt aggaaaatca ttaaggaaga aattaacgac attaaagata cagatgtcat
     3421 catgaagagg aaaagaggag ggagccctgc tgtaacactt cttattagtg aaaaaatatc
     3481 tgtggatata accctggctt tggaatcaaa aagtagctgg cctgctagca cccaagaagg
     3541 cctgcgcatt caaaactggc tttcagcaaa agttaggaag caactacgac taaagccatt
     3601 ttaccttgta cccaagcatg caaaggaagg aaatggtttc caagaagaaa catggcggct
     3661 atccttctct cacatcgaaa aggaaatttt gaacaatcat ggaaaatcta aaacgtgctg
     3721 tgaaaacaaa gaagagaaat gttgcaggaa agattgttta aaactaatga aatacctttt
     3781 agaacagctg aaagaaaggt ttaaagacaa aaaacatctg gataaattct cttcttatca
     3841 tgtgaaaact gccttctttc acgtatgtac ccagaaccct caagacagtc agtgggaccg
     3901 caaagacctg ggcctctgct ttgataactg cgtgacatac tttcttcagt gcctcaggac
     3961 agaaaaactt gagaattatt ttattcctga attcaatcta ttctctagca acttaattga
     4021 caaaagaagt aaagaatttc tgacaaagca aattgaatat gaaagaaaca atgagtttcc
     4081 agtttttgat gaattttacc catacgatgt tccagattac gcttgagtcg acaatcaacc
     4141 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4201 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4261 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4321 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4381 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4441 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4501 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4561 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4621 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4681 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag
     4741 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     4801 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     4861 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     4921 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     4981 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     5041 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     5101 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     5161 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     5221 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     5281 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5341 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     5401 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5461 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5521 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     5581 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     5641 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     5701 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5761 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5821 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     5881 gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga
     5941 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6001 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6241 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6421 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6481 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6541 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6601 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6661 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6721 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6781 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6901 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6961 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7021 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7081 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7141 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7201 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7621 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8101 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9241 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9301 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9361 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9421 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taa
//
