LOCUS       sequence-255055-        8990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine cGAS-Delta-N (aa148-507)-HA; Puromycin selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS-Delta-N-HA (murine)
FEATURES             Location/Qualifiers
     source          1..8990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2298..2307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3387..3413
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3423..4011
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3476..3496)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3894..3905)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4025..4524
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4060..4083)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4457..4476
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4545..5144
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4545..4564)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5041..5061
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5282..5515
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5587..5708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5624..5643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5678..5697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5748..5883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5903..5922)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5904..5922)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5932..5949)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5932..5948)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5941..5963)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6090..6545
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6177..6196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6387..6408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6571..6675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6676..6744,6745..7536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6894..6913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7707..8295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8196..8215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8449..8466
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8583..8604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8619..8624,8625..8642,8643..8649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8657..8673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8662..8684
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8681..8697
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8681..8697
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8716..8736
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8718..8736
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8764..8990
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagagcc accatggaca agctaaagaa ggtgctggac aaattgagat
     2341 tgaaacgcaa agatatctcg gaggcggccg agacggtgaa taaagttgtg gaacgcctgc
     2401 tgcgcagaat gcagaaacgg gagtcggagt tcaaaggtgt ggagcagctg aacactggca
     2461 gctactatga acatgtgaag atttctgctc ctaatgaatt tgatgttatg tttaaactgg
     2521 aagtccccag gattgagcta caagaatatt atgaaacagg tgctttctat cttgtgaaat
     2581 tcaaaagaat tccacgagga aatccgctga gtcatttctt agaaggggaa gtattatcag
     2641 ctaccaagat gctgtcaaag tttaggaaaa tcattaaaga agaagttaaa gaaatcaaag
     2701 atatagatgt cagtgtggag aaggaaaaac caggaagccc tgctgtaaca cttcttatca
     2761 ggaaccctga agaaatctct gtggatataa ttctggcttt ggagtcaaaa ggcagctggc
     2821 ctattagtac caaagaagga ctacctattc aaggctggct gggcacaaaa gtgaggacca
     2881 atctaagacg agagccgttt tatctcgtac ccaagaatgc aaaggatgga aatagttttc
     2941 aaggagagac ctggcgcctc tctttctctc acactgaaaa gtacattttg aataatcacg
     3001 ggatagagaa aacatgctgt gaatcttccg gagcaaaatg ctgcagaaaa gaatgtttaa
     3061 aattaatgaa ataccttttg gaacagttga aaaaagagtt tcaagagctg gatgcattct
     3121 gttcctacca tgtgaaaact gccatctttc acatgtggac ccaggacccg caggacagtc
     3181 agtgggaccc caggaacctc agctcctgct tcgataagtt gttagcattc tttcttgagt
     3241 gcctcaggac agagaaactg gatcattatt ttattccaaa gttcaatcta ttctctcaag
     3301 aactaattga ccgaaaaagt aaagaatttc tatcgaagaa aattgaatat gaaagaaata
     3361 atgggtttcc aatttttgac aagctttacc catacgatgt tccagattac gcttgagtcg
     3421 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3481 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3541 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3601 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3661 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3721 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3781 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3841 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3901 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3961 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     4021 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     4081 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     4141 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     4201 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     4261 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     4321 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     4381 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     4441 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     4501 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     4561 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     4621 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     4681 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     4741 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     4801 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     4861 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     4921 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4981 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5041 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5101 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     5161 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
     5221 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5281 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5521 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5581 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5641 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5701 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5821 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5881 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     5941 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6001 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6061 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6121 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6181 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6241 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6301 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6361 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6421 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6481 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6541 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6901 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7201 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7561 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7681 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7741 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7801 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7861 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7921 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8041 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8221 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8281 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8341 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8401 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8461 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8521 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8581 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8641 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8701 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     8761 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     8821 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     8881 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     8941 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
//
