LOCUS       sequence-255063-        7287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encoding PPARD cDNA for retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Legrand N, Bretscher CL, Zielke S, Wilke B, Daude M, Fritz B, 
            Diederich WE, Adhikary T
  TITLE     PPARbeta/delta recruits NCOR and regulates transcription 
            reinitiation of ANGPTL4.
  JOURNAL   Nucleic Acids Res. 2019 Oct 10;47(18):9573-9591. doi: 
            10.1093/nar/gkz685.
  PUBMED    31428774
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-10-10- 10"; volume: "47"; issue: 
            "18"; pages: "9573-9591"
COMMENT     Sequence Label: pMSCVbsd-PPARD-T423A-T427D
FEATURES             Location/Qualifiers
     source          1..7287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2990..3013)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3340..3359
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3428..3826
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    4358..4374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4382..4388,4389..4406,4407..4412))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4427..4448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4565..4582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4736..5324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4816..4835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5495..6286,6287..6355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6118..6137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6356..6460)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6528..6546
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6584..6606)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6706..6725
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6919..6941
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7069..7088
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccact agtaacggcc gccagtgtgc
     1441 tggaattcgc ccttaggtgt ggcgccgagg ggagatcagc catggagcag ccacaggagg
     1501 aagcccctga ggtccgggaa gaggaggaga aagaggaagt ggcagaggca gaaggagccc
     1561 cagagctcaa tgggggacca cagcatgcac ttccttccag cagctacaca gacctctccc
     1621 ggagctcctc gccaccctca ctgctggacc aactgcagat gggctgtgac ggggcctcat
     1681 gcggcagcct caacatggag tgccgggtgt gcggggacaa ggcatcgggc ttccactacg
     1741 gtgttcatgc atgtgagggg tgcaagggct tcttccgtcg tacgatccgc atgaagctgg
     1801 agtacgagaa gtgtgagcgc agctgcaaga ttcagaagaa gaaccgcaac aagtgccagt
     1861 actgccgctt ccagaagtgc ctggcactgg gcatgtcaca caacgctatc cgttttggtc
     1921 ggatgccgga ggctgagaag aggaagctgg tggcagggct gactgcaaac gaggggagcc
     1981 agtacaaccc acaggtggcc gacctgaagg ccttctccaa gcacatctac aatgcctacc
     2041 tgaaaaactt caacatgacc aaaaagaagg cccgcagcat cctcaccggc aaagccagcc
     2101 acacggcgcc ctttgtgatc cacgacatcg agacattgtg gcaggcagag aaggggctgg
     2161 tgtggaagca gttggtgaat ggcctgcctc cctacaagga gatcagcgtg cacgtcttct
     2221 accgctgcca gtgcaccaca gtggagaccg tgcgggagct cactgagttc gccaagagca
     2281 tccccagctt cagcagcctc ttcctcaacg accaggttac ccttctcaag tatggcgtgc
     2341 acgaggccat cttcgccatg ctggcctcta tcgtcaacaa ggacgggctg ctggtagcca
     2401 acggcagtgg ctttgtcacc cgtgagttcc tgcgcagcct ccgcaaaccc ttcagtgata
     2461 tcattgagcc taagtttgaa tttgctgtca agttcaacgc cctggaactt gatgacagtg
     2521 acctggccct attcattgcg gccatcattc tgtgtggaga ccggccaggc ctcatgaacg
     2581 ttccacgggt ggaggctatc caggacacca tcctgcgtgc cctcgaattc cacctgcagg
     2641 ccaaccaccc tgatgcccag tacctcttcc ccaagctgct gcagaagatg gctgacctgc
     2701 ggcaactggt caccgagcac gcccagatga tgcagcggat caagaaggcc gaaaccgagg
     2761 actcgctgca ccctctgctc caggagatct acaaggacat gtactaacgg cggcacccag
     2821 gcctccctgc agactccaat ggggccagca ctggaggggc ccacccacat gacttttcca
     2881 ttgaccagct ctcaagggcg aattctgcag atatccatca cactggcggc cgctcgaggt
     2941 taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     3001 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     3061 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     3121 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     3181 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     3241 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     3301 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3361 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     3421 gcttaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     3481 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     3541 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     3601 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     3661 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     3721 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     3781 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaatcga taaaataaaa
     3841 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     3901 gctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     3961 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     4021 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     4081 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     4141 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     4201 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     4261 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     4321 atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac
     4381 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4441 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4501 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4561 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5161 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5401 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6061 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6481 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     6541 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6601 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6661 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6721 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6781 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6841 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6901 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
     6961 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     7021 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     7081 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     7141 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     7201 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     7261 tttatttagt ctccagaaaa agggggg
//
