LOCUS       sequence-255070-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encoding PPARD cDNA for retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Legrand N, Bretscher CL, Zielke S, Wilke B, Daude M, Fritz B, 
            Diederich WE, Adhikary T
  TITLE     PPARbeta/delta recruits NCOR and regulates transcription 
            reinitiation of ANGPTL4.
  JOURNAL   Nucleic Acids Res. 2019 Oct 10;47(18):9573-9591. doi: 
            10.1093/nar/gkz685.
  PUBMED    31428774
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-10-10- 10"; volume: "47"; issue: 
            "18"; pages: "9573-9591"
COMMENT     Sequence Label: pMSCVbsd-PPARD-E435A
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2990..3013)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3352..3371
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3440..3838
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    4370..4386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4394..4400,4401..4418,4419..4424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4439..4460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4577..4594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4748..5336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4828..4847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5507..6298,6299..6367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6130..6149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6368..6472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6540..6558
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6596..6618)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6718..6737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6931..6953
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7081..7100
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccact agtaacggcc gccagtgtgc
     1441 tggaattcgc ccttaggtgt ggcgccgagg ggagatcagc catggagcag ccacaggagg
     1501 aagcccctga ggtccgggaa gaggaggaga aagaggaagt ggcagaggca gaaggagccc
     1561 cagagctcaa tgggggacca cagcatgcac ttccttccag cagctacaca gacctctccc
     1621 ggagctcctc gccaccctca ctgctggacc aactgcagat gggctgtgac ggggcctcat
     1681 gcggcagcct caacatggag tgccgggtgt gcggggacaa ggcatcgggc ttccactacg
     1741 gtgttcatgc atgtgagggg tgcaagggct tcttccgtcg tacgatccgc atgaagctgg
     1801 agtacgagaa gtgtgagcgc agctgcaaga ttcagaagaa gaaccgcaac aagtgccagt
     1861 actgccgctt ccagaagtgc ctggcactgg gcatgtcaca caacgctatc cgttttggtc
     1921 ggatgccgga ggctgagaag aggaagctgg tggcagggct gactgcaaac gaggggagcc
     1981 agtacaaccc acaggtggcc gacctgaagg ccttctccaa gcacatctac aatgcctacc
     2041 tgaaaaactt caacatgacc aaaaagaagg cccgcagcat cctcaccggc aaagccagcc
     2101 acacggcgcc ctttgtgatc cacgacatcg agacattgtg gcaggcagag aaggggctgg
     2161 tgtggaagca gttggtgaat ggcctgcctc cctacaagga gatcagcgtg cacgtcttct
     2221 accgctgcca gtgcaccaca gtggagaccg tgcgggagct cactgagttc gccaagagca
     2281 tccccagctt cagcagcctc ttcctcaacg accaggttac ccttctcaag tatggcgtgc
     2341 acgaggccat cttcgccatg ctggcctcta tcgtcaacaa ggacgggctg ctggtagcca
     2401 acggcagtgg ctttgtcacc cgtgagttcc tgcgcagcct ccgcaaaccc ttcagtgata
     2461 tcattgagcc taagtttgaa tttgctgtca agttcaacgc cctggaactt gatgacagtg
     2521 acctggccct attcattgcg gccatcattc tgtgtggaga ccggccaggc ctcatgaacg
     2581 ttccacgggt ggaggctatc caggacacca tcctgcgtgc cctcgaattc cacctgcagg
     2641 ccaaccaccc tgatgcccag tacctcttcc ccaagctgct gcagaagatg gctgacctgc
     2701 ggcaactggt caccgagcac gcccagatga tgcagcggat caagaagacc gaaaccgaga
     2761 cctcgctgca ccctctgctc caggcgatct acaaggacat gtactaacgg cggcacccag
     2821 gcctccctgc agactccaat ggggccagca ctggaggggc ccacccacat gacttttcca
     2881 ttgaccagct ctcaagggcg aattctgcag atatccatca cactggcggc cgctcgaggt
     2941 taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     3001 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     3061 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     3121 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     3181 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     3241 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     3301 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3361 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3421 cctgcagccc aagcttacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     3481 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     3541 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     3601 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     3661 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     3721 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     3781 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaatc
     3841 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     3901 taggtttggc aagctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     3961 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     4021 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     4081 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     4141 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     4201 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
     4261 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
     4321 aaatagttca ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc
     4381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4501 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5161 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6481 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6541 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     6601 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6661 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     6721 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6781 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     6841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     6901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc
     6961 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     7021 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     7081 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     7141 gatgcgtccg gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca
     7201 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     7261 aaaataaaag attttattta gtctccagaa aaagggggg
//
