LOCUS       sequence-255078-        6809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the voltage sensor QuasAr in BWMs of C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, 
            Bach M, Liewald JF, Gottschalk A
  TITLE     Rhodopsin-based voltage imaging tools for use in muscles and neurons
            of Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116.
  PUBMED    31371514
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116."
COMMENT     Sequence Label: pAB16 (pmyo-3::QuasAr)
FEATURES             Location/Qualifiers
     source          1..6809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    85..106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(121..126,127..144,145..151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    159..175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     164..186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     183..199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     183..199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4577..4599)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4699..4718
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4796..4814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4882..4986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4987..5055,5056..5847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5205..5224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6018..6606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6507..6526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6760..6777
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      121 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      181 cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta
      241 actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat
      301 ggatacgcta acaacttgga aatgaaataa gcttgggctg caggtcggct ataataagtt
      361 cttgaataaa ataattttcc cgacaaaaca tgagtatttc tttcgaaaat aaaagtgcag
      421 gctaattaga gattattctg taattaactg cataatttgt cacgtgccat agttttacat
      481 tccactacgt catagttctt aaaatactaa tctcctgaaa atagaagtag gtgaagaaag
      541 tttaattatc agttctaaaa tgacaattga tctttggaat atgttctgaa actaccgatc
      601 attgaacaga tgctatttga atgatataga attgtatatt tgcaatttct gaaacgcgtt
      661 cttaaaggca cacagattaa ttcaaaaggg tctggccgca aaaaggttta tggtggccga
      721 ttttgagttt tgtgtgtgat tgctttttca caatcagtgt tttcaggatt atgtgatgaa
      781 ctagatcttc aagtttcgtt acatttcata tgttttcgga actcacgaag tacatattgg
      841 gtattgtgct caaaaaattc agcaatcagc ttcgctccgc tgactttaga acccaaaaaa
      901 atagtatggc caaactgact gtgttacgat catttcaatt tttcaataca tatttaagat
      961 ttctaagagt aagaaggtca aaaactgttc tggaatacat atatattttt caggttacaa
     1021 ttagtcaaaa agtgcactga aatatacgtt ttaatttcac gaataaccca attagttcaa
     1081 tgtatttttg gtcaaccaac gttaaagttt ggcttccaac caattatcat ttctgatcaa
     1141 ccacaatgtt ttttctttat ctgcaagtta attttttatt tttatccaga tgtttggcat
     1201 atttttcaat tcttcactag cgcccacttc ttgcacttcc ggcgccctga atctaatgca
     1261 tctgttgcaa gaattgaaag accaatcaac acattgtttt cttcacgaga tactgaagaa
     1321 aatgaataaa aacagagaaa aagagccatg tgattagtga caactgttgc taacagatac
     1381 catagcttgg acttggtacg tgatggcaac gtatgggtca acaaaaatga ttgcagaggg
     1441 ggtgcaaaac agtcaagtcg agaaaatatg aaaaacagaa aacaaagaac agaaaaatgg
     1501 gtttgagagt cagtataatt tataaaagaa aaattgtaca tagaaattaa ccatttttgt
     1561 agaagaagtt atttttcaag catcgttaaa aattattcaa agcaccttat ttcatattta
     1621 attttaaaca tggttaaatg aacaacacgg tgcgcaatca ggaaaacttg aaatctgaaa
     1681 ctgttgttgt gatcttcttc gcaactgttc agatagcact agtgtaatgt taagagtgcg
     1741 cgaatataat ggaatataat ggatcacacc tcctgccatc aggtaaacgt ctctgttatc
     1801 acatatttcc aactattaaa tttttacctt ttacagtttt acattttttt gaaaaaagta
     1861 actttttgtc ttcaaaatcc ctgacgaaaa tatcaaatat tttaatcgag actgcagagg
     1921 aaccgattga tgatttggaa aatccagctt tacctgtgta agaactgaaa agtttcataa
     1981 ccctagggta ttcccagtta cattccccac tggctaacaa tagcacccag tttttcatca
     2041 cctttcttca aatttctcgg cgatttgtta aaaacaaaat ttgtgtccct tctctgatat
     2101 ctctatgtct ctaaacacaa gttcatcgga aaacgaagga gggtaggtgt tggttgggct
     2161 cccgaagtga aaatagaaga gcaagaatag aatattagag agagagtgca gagagggcgg
     2221 gatagctccc gggattccgt tttcttcttc tttatcttca acgatgatgt gtgtgcgtgt
     2281 tgtatagatt ctgttgctcc cccacaactc gctccgaagg ctcaatacaa ttcaattgat
     2341 attggaggag agcctaccgg agtgggagga taagaagaaa cataagaaga agaagaagaa
     2401 gaagcatgct tctggttttt gatgctatga aaacggcaca aaaagatgat tgaggtccct
     2461 tttcaatacc ttctctcatc tttcaaatcc cattgaaacc taaaacttct caccacgctt
     2521 taccattgtt ctccaaaaac ttatagcaat gtctataact tttttatctc tgaaaagcag
     2581 tgttccattt ttctttttcc tattttattt caattgtttc tcacatttcg tttggattct
     2641 ttgcttgtca accagcttct tcttccactt ttaccgtcta attttcaggg cagggagcca
     2701 tcaaacccac gaccactaga tccatatggt aagtatcgct ctgcaggctg gttacgacct
     2761 actgggtgac ggcagacctg aaaccctgtg gctgggcatc ggcactctgc tgatgctgat
     2821 tggaaccttc tactttctgg tccgcggatg gggagtcacc gataaggatg cccgggaata
     2881 ttacgctgtg actatcctgg tgtccggaat cgcatccgcc gcatatctgt ctatgttctt
     2941 tggtatcggg cttactgagg tgtccgtcgg gggcgaaatg ttggatatct attatgccag
     3001 gtacgcccag tggctgttta ccaccccact tctgctgctg cacctggccc ttctcgctaa
     3061 ggtggatcgg gtgaccatcg gcaccctggt gggtgtggac gccctgatga tcgtcactgg
     3121 cctcatcgga gccttgagcc acacggccat agccagatac agttggtggt tgttctctac
     3181 aatttgcatg atagtggtgc tctatgttct ggctacatcc ctgcgatctg ctgcaaagga
     3241 gcggggcccc gaggtggcat ctacctttaa caccctgaca gctctggtct tggtgctgtg
     3301 gaccgcttac cctatcctgt ggatcatagg cactgagggc gctggcgtgg tgggcctggg
     3361 catcgaaact ctgctgttta tggtgttgga cgtgactgcc aaggtcggct ttggctttat
     3421 cctgttgaga tcccgggcta ttctgggcga caccgagccc gggattggcc aaaggaccca
     3481 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta
     3541 gcgtcgacgg taccggtcag atatcactcg aggagctccc gagatcttca gcgctcagca
     3601 ttcgtagaat tgtaagttta aactttctca tactaactaa cgcgctctat ttaaattttc
     3661 agaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     3721 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     3781 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     3841 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     3901 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     3961 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     4021 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     4081 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     4141 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     4201 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     4261 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     4321 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     4381 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     4441 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     4501 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     4561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4681 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4741 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     4801 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5221 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5521 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5881 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5941 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6001 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6061 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6781 gcggaagagc gcccaatacg caaaccgcc
//
