LOCUS       sequence-255079-        5686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the eFRET-based voltage sensor QuasAr::mOrange in the 
            pharynx of C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, 
            Bach M, Liewald JF, Gottschalk A
  TITLE     Rhodopsin-based voltage imaging tools for use in muscles and neurons
            of Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116.
  PUBMED    31371514
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116."
COMMENT     Sequence Label: pNH13 (pmyo-2::QuasAr::mOrange)
FEATURES             Location/Qualifiers
     source          1..5686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3606..3628)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3728..3747
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3825..3843)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3911..4015
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4016..4084,4085..4876)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4234..4253)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5047..5635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5536..5555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcacatgt caggtcgagg catttgaatt gggggtggtg gacagtaact gtctgtaata
       61 ataattactc ctgaccaggt tgcaattcga gttttgataa gcataattat accttgtaca
      121 ttgtgggttt tgtgctgtgg acgttttatt gtgacatccc cataagctac aagaacaaaa
      181 atgaaatgta aaagtattga aaacgtcgta cattttatat ctgagtagta tcctttgctt
      241 taaatgtcca taaaactaat tttataatca ataaaacaac gtttgtaaat caactgagtt
      301 tacaagtaga gacattgagg gatactttca ctatgctaaa gtgataatcg accaaataat
      361 aacttcactt tggtatttat tcctgtctta taaatgttat gtatgaatta aattcatatg
      421 catatggctc actctgacaa tttttaataa tcttccagat caatattgac taccgatgcg
      481 ggtggtcttt tgctttgaat tctgctgaac tttacacccc gaacagcaat gtgtgcttca
      541 gcctaaaaaa aagtaagtgt gttaatcagt gcccccgatt cttcattttt tgcccctctc
      601 tcccgtttcg tcggcaaaag aagagaaaat aaagataagt ctcaagatag gttggtaatc
      661 gctaaagtgg ttgtgtggat aagagtagca aaatggcagg aagagcactt tgcgcgcaca
      721 cactgtactc attgttctgg ataaaattct ctcgttgttt gccgtcggat gtctgcctct
      781 ctgcattgag ccggcttctt cactatcttt agttaaccta aaatgccgtt tcttttctcg
      841 tatccccact atcccgttga ggttctctgc tctcttcgct ccctaccgcc agcgagcaac
      901 tatccgtggg ggcgccttgc tcggaagatg ggggggaaga aagaagattt ttgctatttg
      961 cacttgagaa agagactttt cctgcgtcga tggttagaga acagtgtgca gacacttttc
     1021 agctacctag aattacaatt ggatatcccc gcctcccaat ccacccaccc agggaaaaag
     1081 aagggctcgc cgaaaatcaa agttatctcc aggctcgcgc atcccaccga gcggttgact
     1141 tctctccacc acttttcatt ttaaccctcg ggggatccac catggtaagt atcgctctgc
     1201 aggctggtta cgacctactg ggtgacggca gacctgaaac cctgtggctg ggcatcggca
     1261 ctctgctgat gctgattgga accttctact ttctggtccg cggatgggga gtcaccgata
     1321 aggatgcccg ggaatattac gctgtgacta tcctggtgtc cggaatcgca tccgccgcat
     1381 atctgtctat gttctttggt atcgggctta ctgaggtgtc cgtcgggggc gaaatgttgg
     1441 atatctatta tgccaggtac gcccagtggc tgtttaccac cccacttctg ctgctgcacc
     1501 tggcccttct cgctaaggtg gatcgggtga ccatcggcac cctggtgggt gtggacgccc
     1561 tgatgatcgt cactggcctc atcggagcct tgagccacac ggccatagcc agatacagtt
     1621 ggtggttgtt ctctacaatt tgcatgatag tggtgctcta tgttctggct acatccctgc
     1681 gatctgctgc aaaggagcgg ggccccgagg tggcatctac ctttaacacc ctgacagctc
     1741 tggtcttggt gctgtggacc gcttacccta tcctgtggat cataggcact gagggcgctg
     1801 gcgtggtggg cctgggcatc gaaactctgc tgtttatggt gttggacgtg actgccaagg
     1861 tcggctttgg ctttatcctg ttgagatccc gggctattct gggcgacacc gagttgagga
     1921 acatggccat catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
     1981 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc tttcagaccg
     2041 ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2101 atttcaccta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttca
     2161 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaactacgag gacggcggcg
     2221 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2281 tgcgcggcac caacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg
     2341 aggcctcctc cgagcggatg taccccgagg acggtgccct gaagggcaag atcaagatga
     2401 ggctgaagct gaaggacggc ggccactaca cctccgaggt caagaccacc tacaaggcca
     2461 agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcacctccc
     2521 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2581 gcggcatgga cgagctgtac aagagtagaa tcacaagcga aggcgagtac atccccctgg
     2641 atcaaataga cataaatgta ggtggatttt gttatgagaa tgaagtataa gaattccaac
     2701 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt
     2761 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca
     2821 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     2881 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     2941 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     3001 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     3061 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     3121 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     3181 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     3241 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     3301 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     3361 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     3421 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     3481 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     3541 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     3601 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     3661 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     3721 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     3781 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     3841 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3901 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3961 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4021 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4081 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4141 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4201 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4261 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4321 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4381 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4441 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4501 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4561 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4621 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4681 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4741 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4801 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4861 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4921 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4981 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5041 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5101 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5161 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5221 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5281 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5341 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5401 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5461 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5521 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5581 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5641 cagcaacgcg gcctttttac ggttcctggc cttttgctgg cctttt
//
