LOCUS       sequence-255081-        5827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the eFRET-based voltage sensor MacQ::mCitrine in the 
            pharynx of C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, 
            Bach M, Liewald JF, Gottschalk A
  TITLE     Rhodopsin-based voltage imaging tools for use in muscles and neurons
            of Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116.
  PUBMED    31371514
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116."
COMMENT     Sequence Label: pNH12 (pmyo-2::MacQ::mCitrine)
FEATURES             Location/Qualifiers
     source          1..5827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2102..2123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2363..2382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2522..2773
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2710..2731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3776..3798)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3898..3917
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3995..4013)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4081..4185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4186..4254,4255..5046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4404..4423)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5217..5805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5706..5725
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggttcct ggccttttgc tggccttttg ctcacatgtc aggtcgaggc atttgaattg
       61 ggggtggtgg acagtaactg tctgtaataa taattactcc tgaccaggtt gcaattcgag
      121 ttttgataag cataattata ccttgtacat tgtgggtttt gtgctgtgga cgttttattg
      181 tgacatcccc ataagctaca agaacaaaaa tgaaatgtaa aagtattgaa aacgtcgtac
      241 attttatatc tgagtagtat cctttgcttt aaatgtccat aaaactaatt ttataatcaa
      301 taaaacaacg tttgtaaatc aactgagttt acaagtagag acattgaggg atactttcac
      361 tatgctaaag tgataatcga ccaaataata acttcacttt ggtatttatt cctgtcttat
      421 aaatgttatg tatgaattaa attcatatgc atatggctca ctctgacaat ttttaataat
      481 cttccagatc aatattgact accgatgcgg gtggtctttt gctttgaatt ctgctgaact
      541 ttacaccccg aacagcaatg tgtgcttcag cctaaaaaaa agtaagtgtg ttaatcagtg
      601 cccccgattc ttcatttttt gcccctctct cccgtttcgt cggcaaaaga agagaaaata
      661 aagataagtc tcaagatagg ttggtaatcg ctaaagtggt tgtgtggata agagtagcaa
      721 aatggcagga agagcacttt gcgcgcacac actgtactca ttgttctgga taaaattctc
      781 tcgttgtttg ccgtcggatg tctgcctctc tgcattgagc cggcttcttc actatcttta
      841 gttaacctaa aatgccgttt cttttctcgt atccccacta tcccgttgag gttctctgct
      901 ctcttcgctc cctaccgcca gcgagcaact atccgtgggg gcgccttgct cggaagatgg
      961 gggggaagaa agaagatttt tgctatttgc acttgagaaa gagacttttc ctgcgtcgat
     1021 ggttagagaa cagtgtgcag acacttttca gctacctaga attacaattg gatatccccg
     1081 cctcccaatc cacccaccca gggaaaaaga agggctcgcc gaaaatcaaa gttatctcca
     1141 ggctcgcgca tcccaccgag cggttgactt ctctccacca cttttcattt taaccctcgg
     1201 gggatccgcc accatgatcg tggaccagtt cgaggaggtg ctgatgaaga ccagccagct
     1261 gttcccactg ccaaccgcta cccagagcgc ccagccaacc cacgtggccc ccgtgccaac
     1321 cgtgctgccc gacaccccca tctacgagac cgtgggcgac agcggcagca agaccctgtg
     1381 ggtggtgttc gtgctgatgc tgatcgccag cgccgccttc accgccctga gctggaagat
     1441 ccccgtgaac aggaggctgt accacgtgat caccaccatc atcaccctga ccgccgccct
     1501 gagctacttc gctatggcta ccggccacgg agtggccctg aacaagatcg tgatcaggac
     1561 ccagcacgac cacgtgcccg acacctacga gaccgtgtac cgacaggtgt actacgccag
     1621 gtacatccag tgggctatca ccaccccact gctgctgctg gacctgggac tgctggctgg
     1681 aatgagcgga gcccacatct tcatggccat cgtggctgac ctgatcatgg tgctgaccgg
     1741 cctgttcgct gctttcggca gcgagggaac cccacagaag tggggatggt acaccatcgc
     1801 ctgcatcgcc tacatcttcg tggtgtggca cctggtgctg aacggcggcg ccaacgccag
     1861 ggtgaagggc gagaagctga ggagcttctt cgtggccatc ggagcttaca ccctgatcct
     1921 gtggaccgct tacccaatcg tgtggggact ggctgacgga gctaggaaga tcggagtgga
     1981 cggagagatc atcgcttacg ctgtgctgga cgtgctggct aagggagtgt tcggagcttg
     2041 gctgctggtg acccacgcca acctgtccgg agaagagctg tttaccgggg tggtgcccat
     2101 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2161 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2221 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcgcgcta
     2281 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2341 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2401 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2461 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2521 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2581 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2641 gctgcccgac aaccactacc tgagctacca gtccaaactg agcaaagacc ccaacgagaa
     2701 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2761 cgagctgtac aagaagagca ggatcaccag cgagggcgag tacatccccc tggaccagat
     2821 cgacatcaac gtgttctgct acgagaacga ggtgtaatga gaattccaac tgagcgccgg
     2881 tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta
     2941 agtttaaact gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc
     3001 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     3061 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     3121 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     3181 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     3241 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     3301 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     3361 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3421 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3481 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     3541 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     3601 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     3661 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     3721 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     3781 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     3841 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     3901 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     3961 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     4021 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4681 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4741 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4801 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4861 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5161 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5221 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5281 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5341 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5401 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5461 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5521 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5581 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5641 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5701 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5761 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5821 gcctttt
//
