LOCUS       sequence-25512-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC219
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..44)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(66..86)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(150..170)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    246..293
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    303..327
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctttactca ttttttctac cggtacctta cgcttcttct ttggagcagt cattttttct
       61 gccggtacct tacgcttctt ctttggagca gtcatttttt ctgccggtac cttacgcttc
      121 ttctttggag cagtcatttt ttctgccggt accttacgct tcttctttgg agcagtcatt
      181 ttttctgagc tcctgaaaat gttctatgtt atgttagtat cattcgaaac atacccgggg
      241 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcat
      301 caaccacttt gtacaagaaa gctgggtaat aaaattgtgt aagatgacgc tataaaaaat
      361 gacgagctgt gagccaagta ggagttgagt aatgaaaaga aaatgacaga tgtaactgac
      421 cctttaccat ctaaaaaagg aatcgtgtgc cgtcccaaca gccacctgct tcacacatgt
      481 tacccttcta ctgacattgt cgagacaaag tcggtaaata attggaacat gatgatattg
      541 ctcataagtt tatagatgga aagagatttc gttgttattt tgcaagaaaa agggaatttg
      601 atgtggtttc
//
