LOCUS       sequence-255126-        8443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is
            inserted within the C-peptide and is co-secreted with insulin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Kalwat MA, Wichaidit C, Nava Garcia AY, McCoy MK, McGlynn K, Hwang 
            IH, MacMillan JB, Posner BA, Cobb MH
  TITLE     Insulin promoter-driven Gaussia luciferase-based insulin secretion 
            biosensor assay for discovery of beta-cell glucose-sensing pathways.
  JOURNAL   ACS Sens. 2016 Oct 28;1(10):1208-1212. Epub 2016 Oct 12.
  PUBMED    27819058
REFERENCE   2  (bases 1 to 8443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens.";
            date: "2016-10-28"; volume: "1(10)"; pages: "1208-1212. Epub 2016 
            Oct 12"
COMMENT     Sequence Label: pLenti-rInsp-hIns-GLuc
FEATURES             Location/Qualifiers
     source          1..8443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(1930..1950)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1935..2264
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2115..2250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2274..2873
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2274..2293)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2770..2790
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     sig_peptide     3369..3440
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
     CDS             4224..4241
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4283..4871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4336..4356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4754..4765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4962..5195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5267..5388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5304..5323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5358..5377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5428..5563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5583..5602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5584..5602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5612..5629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5612..5628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5621..5643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5770..6225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5857..5876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6067..6088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6251..6355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6356..6424,6425..7216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6574..6593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7387..7975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7876..7895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8129..8146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8263..8284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8299..8304,8305..8322,8323..8329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8337..8353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8342..8364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8361..8377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8361..8377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8396..8416
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8398..8416
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     1981 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2041 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2101 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2221 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg
     2281 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     2341 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     2401 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     2461 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     2521 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     2581 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     2641 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     2701 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     2761 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     2821 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     2881 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgctgagct
     2941 aagaatccag ctatcaatag aaactatgaa acagttccag ggacaaagat accaggtccc
     3001 caacaactgc aactttctgg gaaatgaggt ggaaaatgct cagccaagga aaaagagggc
     3061 cttaccctct ctgggacaat gattgtgctg tgaactgctt catcaggcca tctggcccct
     3121 tgttaataat ctaattaccc taggtctaag tagagttgtt gacgtccaat gagcgctttc
     3181 tgcagactta gcactaggca agtgtttgga aattacagct tcagcccctc tcgccatctg
     3241 cctacctacc cctcctagag cccttaatgg gccaaacggc aaagtccagg gggcagagag
     3301 gaggtgcttt ggactataaa gctagtggag acccagtaac tcccaagcta gcgtttaaac
     3361 ttaagcttat ggccctgtgg atgcgcctcc tgcccctgct ggcgctgctg gccctctggg
     3421 gacctgaccc agccgcagcc tttgtgaacc aacacctgtg cggctcacac ctggtggaag
     3481 ctctctacct agtgtgcggg gaacgaggct tcttctacac acccaagacc cgccgggagg
     3541 cagaggacct gcaggtgggg caggtggagc tgggcggggg ccctggatcc aagcccaccg
     3601 agaacaacga agacttcaac atcgtggccg tggccagcaa cttcgcgacc acggatctcg
     3661 atgctgaccg cgggaagttg cccggcaaga agctgccgct ggaggtgctc aaagagatcg
     3721 aagccaatgc ccggaaagct ggctgcacca ggggctgtct gatctgcctg tcccacatca
     3781 agtgcacgcc caagatgaag aagttcatcc caggacgctg ccacacctac gaaggcgaca
     3841 aagagtccgc acagggcggc ataggcgagg cgatcgtcga cattcctgag attcctgggt
     3901 tcaaggactt ggagcccatc gagcagttca tcgcacaggt cgatctgtgt gtggactgca
     3961 caactggctg cctcaaaggg cttgccaacg tgcagtgttc tgacctgctc aagaagtggc
     4021 tgccgcaacg ctgtgcgacc tttgccagca agatccaggg ccaggtggac aagatcaagg
     4081 gggccggtgg tgacgaattc ggtgcaggca gcctgcagcc cttggccctg gaggggtccc
     4141 tgcagaagcg tggcattgtg gaacaatgct gtaccagcat ctgctccctc taccagctgg
     4201 agaactactg caactagctc gagcaccacc atcaccatca ccaggatcct gcagtctaga
     4261 gctagcatat gaattcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     4321 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4381 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4441 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4501 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4561 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4621 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4681 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4741 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4801 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4861 cgcctccccg cctggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc
     4921 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     4981 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5041 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5101 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5161 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     5221 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     5281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     5401 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5461 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5521 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     5581 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     5641 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     5701 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     5761 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5821 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5881 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5941 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6001 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6061 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6121 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6181 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6241 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6301 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6361 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6421 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6481 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6541 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6601 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6661 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6721 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6781 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6841 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6901 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6961 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7021 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7081 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7141 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7201 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7261 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7321 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7381 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7441 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7501 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7561 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7621 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7681 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7741 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7801 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7861 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7921 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7981 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8041 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8101 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8161 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8221 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8281 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8341 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     8401 taaccctcac taaagggaac aaaagctgga gctgcaagct taa
//
