LOCUS       sequence-255131-        8647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung 
            R, de March C, Bloom JS, Matsunami H, Kosuri S
  TITLE     A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions 
            with RNA Sequencing.
  JOURNAL   Cell Syst. 2019 Mar 27;8(3):254-260.e6. doi: 
            10.1016/j.cels.2019.02.009. Epub 2019 Mar 20.
  PUBMED    30904378
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2019.02.009"; journalName: "Cell Syst"; date: 
            "2019-03-27- 27"; volume: "8"; issue: "3"; pages: "254-260.e6"
COMMENT     Sequence Label: pB-Ric8b-Integration
FEATURES             Location/Qualifiers
     source          1..8647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1406..1424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1443..1461
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1480..1498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1517..1535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1554..1572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1591..1609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1628..1646
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1665..1683
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1705..1729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1813..1822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3732..3866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3977..4188
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4201..4469
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4496..5095
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4496..4515)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4992..5012
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5155..5175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5217..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6101..6135
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6626..6642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6626..6642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6639..6661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6650..6666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6674..6680,6681..6698,6699..6704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6719..6740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6857..6874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7028..7616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7108..7127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7787..8578,8579..8647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8410..8429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtccaatcat aggggtaccc
     1381 gacccatcaa gtgccacctg acgtctccct atcagtgata gagaagtcga cacgtctcga
     1441 gctccctatc agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag
     1501 tcgacacgtc tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat
     1561 cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta
     1621 gaacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag
     1681 agaaggtacc ccctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     1741 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tgagctcgtt
     1801 tagtgaccgg ccgccaccat ggatgaagag cgcgccctct acatcgtccg ggccggcgag
     1861 gcgggggcta tcgagcgggt cctgagggat tacagcgaca agcatagggc tactttcaaa
     1921 tttgaatcag cagatgaaga taaaagaaag aaactctgtg aaggcatatt taaggtcctt
     1981 gtcaaggagg tcccaacgac atgccaagtg tcctgcctgg aagtactccg cattctctcc
     2041 agagacaaaa agatcttagt tcctgtaaca actaaggaaa atatgcaaat actgctgaga
     2101 ctagccaagc tccacgagtc agacgattct ttggagaagg tgtcagagtt cccagttatt
     2161 gtggaatcat tgaagtgtct gtgtaacatc gtgttcaaca gtcagatggc acagcagctt
     2221 agcctggaac tgaatctcgc tgcaaagctc tgtaacctcc taaggaagtg caaggaccga
     2281 aaatttatca atgacataaa gtgctttgac ttgcgcttgc tcttcgtctt gtcacttttg
     2341 cacactgaca tcaggtcaca attgcgctat gagctccagg gactcccgct gctaacccag
     2401 atcttggaaa gtgcctttag catcaagtgg accgatgagt atgagtcggc catagaccat
     2461 aatggacctc ctctctcacc tcaggagaca gactgtgcca ttgaggccct caaagctctg
     2521 ttcaatgtga cggtagacag ttggaaggtg cataaagaga gcgattctca tcagtttcgt
     2581 gtcatggcag ctgtccttcg ccattgttta ctaattgtag gtccaactga agacaaaacc
     2641 gaagagctgc acagtaatgc cgtcaacctt ttaagcaatg ttccagtctc ttgtttggat
     2701 gttctcattt gtccattaac ccatgaagaa acagcccaag aggcagcaac tctagatgaa
     2761 ctgcccagtg ataaaacaac ggagaaagac acagctttga aaaacagtac catggtatac
     2821 aatggcatga atatggaggc cattcacgtt ttactcaatt ttatggagaa gagaattgat
     2881 aagggaagca gctatagaga gggtctaact ccagttctca gcttattaac agaatgttcc
     2941 cgagcccatc ggaacatccg aaaatttctc aaagatcagg ttttaccgcc tttgagagat
     3001 gtgacaaatc gaccagaagt cggctcaacc gtgagaaata agctggttcg tctcatgaca
     3061 catgttgacc tcggagtcaa gcagattgct gctgagttcc tttttgtcct ttgcaaagag
     3121 agagtggata gcctgctgaa atacactggc tatgggaacg ctgcaggact cttggcggcc
     3181 aggggcctct tggctggagg aagaggagat aattggtact cagaggacga ggacacggac
     3241 actgaagaat acaaaaacgc aaaaccaaac attaatctta tcactggtca tttagaggaa
     3301 ccaatgccaa accctataga tgaaatgaca gaagaacaaa aagaatatga agccatgaaa
     3361 cttgtcaaca tgcttgataa actttccaga gaggagttgc ttaaaccaat gggactaaaa
     3421 cctgacggga caataacgcc tttggaggaa gcactcagcc agtattctgt catcgaagag
     3481 accagctcgg acacagactg agaattcgcc acacaggcat agagtgtctg ctattaataa
     3541 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     3601 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     3661 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3721 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3841 tcaatgtatc ttatcatgtc tggatcccca ggaagctcct ctgtgtcctc ataaacccta
     3901 acctcctcta cttgagagga cattccaatc atagggcggc cgcgaaggat ctgcgatcgc
     3961 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4021 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     4081 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     4141 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     4201 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     4261 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     4321 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     4381 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     4441 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac
     4501 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4561 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4621 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4681 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4741 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4801 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4861 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4921 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4981 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5041 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga
     5101 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcgt taactaaact
     5221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5341 atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct
     5401 tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct
     5461 tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc
     5521 cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg
     5581 cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     5641 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     5701 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     5761 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     5821 gtccctgata tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
     5881 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
     5941 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     6001 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     6061 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     6121 actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg
     6181 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     6241 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     6301 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     6361 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     6421 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     6481 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     6541 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     6601 ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8641 tactcat
//
