LOCUS       sequence-255150-       12030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 12030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: pHWBF06exp
FEATURES             Location/Qualifiers
     source          1..12030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1189..1212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1216..1242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2573..2597)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2969..3148
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3184..4209
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4407..4659
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4752..4776
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5297..6088
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6334..6922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6823..6842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7076..7093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7108..7248
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7174..7193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7334..7356
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7592..7786
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7852..8925)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9354..9983)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11314..11338
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtgtcc
      481 aagggcgagg aggacaacat ggccatcatt aaggagttca tgcgcttcaa ggtccacatg
      541 gagggctccg ttaatgggca tgagttcgag atcgaggggg agggcgaggg gaggccctac
      601 gagggcacac agactgccaa gctgaaggtg acgaagggcg ggcctctccc cttcgcctgg
      661 gacatcctgt cgcctcagtt catgtacggc tctaaggcct acgtgaagca ccccgctgac
      721 attccagatt acctcaagct gagcttcccg gagggcttca agtgggagcg ggttatgaac
      781 ttcgaggacg gcggggtggt cacggtgacc caggactcca gcctccagga tggcgagttc
      841 atctacaagg tcaagctgcg cggcacaaat ttcccttccg acgggccggt tatgcagaag
      901 aagactatgg gctgggaggc ctcgtctgag cggatgtacc cggaggatgg cgcgctcaag
      961 ggggagatta agcagcgcct caagctgaag gacggtggcc actacgatgc tgaggtgaag
     1021 accacgtaca aggccaagaa gccggtccag ctcccaggcg cctacaacgt taatatcaag
     1081 ctggacatta catcgcacaa cgaggattac actatcgtgg agcagtacga gagggccgag
     1141 ggcaggcatt ctacgggcgg gatggacgag ctgtacaagg gcgccgggga ttacaaggac
     1201 gatgacgata aggcgtaccc ttacgacgtc cccgattacg cgggcgccgg gatggcttct
     1261 ccctcattcg cggccgctgc tacctctccg gcgtcatccc cactcacgct ggacgctatc
     1321 ccactggcct caaggcccgc cccaggcgcc gctgcggccc agcggaagcg ctcggtgttc
     1381 ctcctgaacc accggcctca tccggtctcg cccaccccgc ccctgcagcc aaccgctgcg
     1441 gcctccgctc agcacgccag gaggaagaag acctctcatc cacctaggcc caggtggcag
     1501 accgtgctct ccatcgctgc caagaatgcc gctctcctgg cggccctcct gtacctcggc
     1561 gacctggcct ggaggtggag ccacccgccc ccacctagcc cgcccccaga ccgggctgcc
     1621 ctggagggct acgcggctcg cgtcgatgag gttgaggcca gcctcacacg gactttccgc
     1681 atgatccagg tccagctgga ggcggttgac aggaagattg atggcgaagt gggggcggcg
     1741 cggggcgacc tcctggcgct cctggaggat aagaggctgg ctctggagag gcagctcacg
     1801 cgcctggacg ctagggccgg cgagctgggg gatgcgctcg ctggcctgaa gaggatggag
     1861 ttcctccgga aggacgagtt cgagaagttc tgggatgagg tgaagggctc actgggctcc
     1921 tcctccgagt cggaggtgga cctcgatcag gtccgggccc tggcgcgcga gatcgtcatg
     1981 cgcgagattg agaagcacgc tgcggacggc atcgggcggg tggattacgc tgtggcttcc
     2041 ggcgggggca gggttgtgcg gcatagcgag gcctacgttc ccaagagggg cttcatggtg
     2101 tggatgtccg gcgtggacgt tgggcccaag ccagagaaga tgctgcagcc tagcttcggc
     2161 gagcccgggc agtgcttcgc tctccagggc tcaaacgggt tcgtggaggt caagctgaag
     2221 tccggcatca ttccagaggc ggtgaccctg gagcacgttt ctaaggacgt ggcctacgat
     2281 cgctcaacgg cgcctaaggg ctgcagggtc tacgggtggt acgaggagac gcccggcgag
     2341 acccagtcgg ggcatgccgc taagatggcg ctcgctgagt tcgcctacga cctggagaag
     2401 aacaatgttc agacattcga cgtgactgcg ccggatgttg gcgtgatcaa catggtgcgc
     2461 ctcgacttca catcgaatca cggctcatcc cagctcactt gcatctaccg cctgcgggtg
     2521 cacggccatg agcctgtcag ccccggcagc tcggctgggt cgcaggcctg agacccagct
     2581 ttcttgtaca aagtggtgat atcccgcggc catgctagag tccgcaaaaa tcaccagtct
     2641 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga
     2701 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     2761 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     2821 atccagtgac ctgcaggcat gcgacgtcgg gccctctaga ggatccccgg gtaccgcgaa
     2881 ttatcgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     2941 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actgagccgc
     3001 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
     3061 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
     3121 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaac tcgaatcccc
     3181 cctatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     3241 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     3301 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     3361 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     3421 attggggagt tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     3481 ttgcaagacc tgcctgaaac cgaactgccc gctgttcttc agccggtcgc ggaggctatg
     3541 gatgctatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     3601 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     3661 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     3721 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     3781 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     3841 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     3901 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     3961 ccaaggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     4021 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     4081 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     4141 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     4201 aaggaataga gtagatgccg accgaacaag agctgatttc gagaacgcct cagccagcaa
     4261 ctcgcgcgag cctagcaagg caaatgcgag agaacggcct tacgcttggt ggcacagttc
     4321 tcgtccacag ttcgctaagc tcgctcggct gggtcgcggg agggccggtc gcagtgattc
     4381 aggaattaat tccctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa
     4441 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     4501 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     4561 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     4621 ataaattatc gcgcgcggtg tcatctatgt tactagatcg accggcatgc aagctgataa
     4681 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     4741 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     4801 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     4861 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     4921 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     4981 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     5041 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     5101 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     5161 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     5221 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     5281 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     5341 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     5401 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     5461 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     5521 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     5581 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     5641 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     5701 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     5761 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     5821 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     5881 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     5941 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     6001 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     6061 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     6121 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     6181 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     6241 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6481 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7081 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     7141 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7201 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     7261 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     7321 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     7381 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     7441 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     7501 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     7561 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     7621 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     7681 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     7741 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     7801 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     7861 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     7921 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     7981 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     8041 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     8101 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     8161 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     8221 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     8281 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     8341 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     8401 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     8461 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     8521 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     8581 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     8641 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     8701 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     8761 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     8821 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     8881 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     8941 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     9001 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     9061 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     9121 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     9181 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     9241 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     9301 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     9361 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     9421 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     9481 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     9541 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     9601 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     9661 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     9721 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     9781 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     9841 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     9901 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     9961 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10021 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    10081 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    10141 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    10201 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    10261 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    10321 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    10381 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    10441 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    10501 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    10561 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    10621 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    10681 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    10741 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    10801 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    10861 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    10921 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    10981 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    11041 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    11101 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    11161 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg
    11221 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    11281 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
    11341 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctaagctt
    11401 gagctctccc atatggtcga ctagagccaa gctgatctcc tttgccccgg agatcaccat
    11461 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
    11521 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
    11581 acagatggtt agagaggcct acgcggcagg tctcatcaag acgatctacc cgagtaataa
    11641 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
    11701 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
    11761 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
    11821 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
    11881 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
    11941 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
    12001 agatacagtc tcagaagacc aaagggctat
//
