LOCUS       sequence-255153-       11592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: pHWBF07exp
FEATURES             Location/Qualifiers
     source          1..11592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2135..2159)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2531..2710
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2746..3771
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3969..4221
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4314..4338
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4859..5650
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5896..6484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6385..6404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6638..6655
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6670..6810
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6736..6755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6896..6918
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7154..7348
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7414..8487)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8916..9545)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10876..10900
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccgatcctgg tggagctgga cggggatgtc
      541 aacggccaca agttctcagt gtccggcgag ggggagggcg acgcgacata cgggaagctc
      601 actctgaagt tcatttgcac gaccggcaag ctcccggttc cctggccaac cctggtgaca
      661 actctcacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc agagggctac gtgcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagaccag ggcggaggtg aagttcgagg gggacacgct ggtcaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctgggcca caagctggag
      901 tacaactaca attcgcataa cgtttacatc atggctgaca agcagaagaa cggcattaag
      961 gtcaatttca agatccgcca caatattgag gacggctccg tccagctggc cgatcattac
     1021 cagcagaaca caccgatcgg ggacggccct gtcctcctgc ccgataatca ctacctgtct
     1081 actcagtcag ccctctccaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacgg cggccgggat caccctgggc atggacgagc tgtacaaggg ggccggcgat
     1201 tacaaggacg atgacgataa ggcgtacccc tacgacgtcc cagattacgc gggggctggc
     1261 atgctcatgc actacgacgt ggataggtcg gaggacaggc agtccgagga ggtccagtct
     1321 gcgtaccgca gggacagcga tatcggcgag gagatgatca tttccagcga ggagcagaac
     1381 gacgagaatg agtggtcgtg ggtgaaccag gacagggatg gtggcccact ggctgagtct
     1441 gtttcgtctc tccacatgac ccagcaggtg ctggagaatg agatccagaa gctctcggag
     1501 ctggggaagg agctggaggc cgaggagagc acgtcgggca acaaggacca ggatgtcatt
     1561 gttctgccat acgctgaggt ggacatgctc aagctgaatg agaagatgga gcacctggag
     1621 cagaaggtga aggaggtctc taacacaatc agggagaagg acctccggct gtcaaagctc
     1681 cagatcctga ttggcacagc ggacagggcc actgtggagg aggctgctgc gagcattgat
     1741 cagctggaga ccgagttcga gcaccatctg caggagatcc tggaggctga gattcagtac
     1801 ctcgtcatga tgaaggcccg ccagaattgg caggttaggg cggaggacca gatggctctg
     1861 gaggagcaca gggcctcagc tgcgggcgat aacacacgga tgctcctgaa gctccgcgag
     1921 actgagagca agatcgttat gctcaaggag caggtggaca agctggaggt tagggagaag
     1981 gatctctacc ggaccacgga ggtgctgaag atgcagtcac gcaccttcaa ggtctccctc
     2041 ttcggcctgg ttcagctcat catgatgtgc ctcagcctga agatgttctt cgctcaggtg
     2101 tcggcccctt tcgacgaggt tgtgccgacg tgagacccag ctttcttgta caaagtggtg
     2161 atatcccgcg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     2221 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     2281 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     2341 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     2401 atgcgacgtc gggccctcta gaggatcccc gggtaccgcg aattatcgat catgagcgga
     2461 gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt
     2521 ggaactgaca gaaccgcaac gttgaaggag ccactgagcc gcgggtttct ggagtttaat
     2581 gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta
     2641 tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg
     2701 tatccaatta gagtctcata ttcactctca actcgaatcc cccctatgaa aaagcctgaa
     2761 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     2821 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     2881 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     2941 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag
     3001 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     3061 accgaactgc ccgctgttct tcagccggtc gcggaggcta tggatgctat cgctgcggcc
     3121 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     3181 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     3241 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     3301 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     3361 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     3421 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     3481 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccaaggct ccgggcgtat
     3541 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     3601 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     3661 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     3721 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc
     3781 cgaccgaaca agagctgatt tcgagaacgc ctcagccagc aactcgcgcg agcctagcaa
     3841 ggcaaatgcg agagaacggc cttacgcttg gtggcacagt tctcgtccac agttcgctaa
     3901 gctcgctcgg ctgggtcgcg ggagggccgg tcgcagtgat tcaggaatta attccctaga
     3961 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     4021 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     4081 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     4141 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     4201 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     4261 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     4321 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     4381 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     4441 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     4501 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     4561 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     4621 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     4681 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     4741 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     4801 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     4861 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     4921 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     4981 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     5041 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     5101 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     5161 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     5221 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     5281 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     5341 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     5401 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     5461 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     5521 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     5581 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     5641 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     5701 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     5761 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     5821 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5881 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5941 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6001 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6061 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6121 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6181 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6241 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6301 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6361 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6421 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6481 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6541 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6601 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6661 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6721 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     6781 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     6841 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6901 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     6961 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     7021 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     7081 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     7141 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     7201 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     7261 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     7321 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     7381 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     7441 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     7501 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     7561 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     7621 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     7681 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     7741 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     7801 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     7861 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     7921 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     7981 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     8041 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     8101 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     8161 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     8221 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     8281 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     8341 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     8401 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     8461 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     8521 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     8581 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     8641 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     8701 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     8761 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     8821 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     8881 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     8941 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
     9001 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
     9061 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
     9121 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
     9181 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
     9241 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
     9301 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
     9361 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
     9421 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
     9481 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
     9541 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
     9601 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
     9661 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
     9721 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
     9781 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
     9841 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
     9901 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
     9961 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    10021 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    10081 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    10141 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    10201 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    10261 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    10321 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    10381 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    10441 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    10501 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    10561 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    10621 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    10681 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac
    10741 tttaaagtac tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    10801 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
    10861 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
    10921 aaggcgggaa acgacaatct gatccaagct caagctaagc ttgagctctc ccatatggtc
    10981 gactagagcc aagctgatct cctttgcccc ggagatcacc atggacgact ttctctatct
    11041 ctacgatcta ggaagaaagt tcgacggaga aggtgacgat accatgttca ccaccgataa
    11101 tgagaagatt agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc
    11161 ctacgcggca ggtctcatca agacgatcta cccgagtaat aatctccagg agatcaaata
    11221 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
    11281 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
    11341 gcttcataaa ccaaggcaag taatagagat tggagtctct aagaaagtag ttcctactga
    11401 atcaaaggcc atggagtcaa aaattcagat cgaggatcta acagaactcg ccgtgaagac
    11461 tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa
    11521 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
    11581 ccaaagggct at
//
