LOCUS       sequence-255154-       10072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd 
            generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10072)
  TITLE     Emory Custom Cloning Core Plasmids - Oskar Laur
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: plenti guide Thy1.1
FEATURES             Location/Qualifiers
     source          1..10072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        87..1265
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          318..1256
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1213..1233
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1278..1766
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    1782..2370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1835..1855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2253..2264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2442..2675
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2753..2874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2790..2809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2844..2863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2914..3049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3069..3088)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3070..3088)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3098..3115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3098..3114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3107..3129)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3256..3711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3343..3362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3553..3574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3737..3841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3842..3910,3911..4702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4060..4079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4873..5461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5362..5381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5615..5632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5749..5770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5785..5790,5791..5808,5809..5815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5823..5839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5828..5850
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5847..5863
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5847..5863
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5882..5902
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5884..5902
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5930..6156
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6157..6337
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6384..6509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7002..7235
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7420..7464
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7642..7890
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7642..7662
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7813..7832
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9772..9847
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9903..10020
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac ggccaccatg aacccagcca tcagcgtcgc tctcctgctc tcagtcttgc
     1321 aggtgtcccg agggcagaag gtgaccagcc tgacagcctg cctggtgaac caaaaccttc
     1381 gcctggactg ccgccatgag aataacacca aggataactc catccagcat gagttcagcc
     1441 tgacccgaga gaagaggaag cacgtgctct caggcaccct tgggataccc gagcacacgt
     1501 accgctcccg cgtcaccctc tccaaccagc cctatatcaa ggtccttacc ctagccaact
     1561 tcaccaccaa ggatgagggc gactactttt gtgagcttcg cgtatcgggc gcgaatccca
     1621 tgagctccaa taaaagtatc agtgtgtata gagacaagct ggtcaagtgt ggcggcataa
     1681 gcctgctggt tcagaacaca tcctggatgc tgctgctgct gctttccctc tccctcctcc
     1741 aagccctgga cttcatttct ctgtgaacgc gttaagtcga caatcaacct ctggattaca
     1801 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     1861 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     1921 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     1981 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2041 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2101 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2161 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     2221 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2281 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2341 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     2401 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     2461 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     2521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2641 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     2701 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     2761 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2821 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2881 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3001 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     3061 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     3121 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3181 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3241 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3301 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3601 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3661 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     3721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3781 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3841 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3901 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3961 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4021 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4081 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4141 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4201 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4261 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4321 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4381 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4441 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4501 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4561 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4621 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4681 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4741 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4801 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4861 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4921 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4981 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5041 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5101 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5161 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5221 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5281 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5341 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5401 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5461 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5521 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5581 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5641 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5701 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     5761 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5821 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     5881 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     5941 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     6001 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     6061 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     6121 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     6181 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6241 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6301 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6361 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6421 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6481 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6541 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6601 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     6661 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     6721 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     6781 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     6841 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     6901 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     6961 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7021 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7081 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7141 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7201 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7261 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7321 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7381 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7441 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7501 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7561 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7621 ccaaccccga ggggacccag agagggccta tttcccatga ttccttcata tttgcatata
     7681 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
     7741 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     7801 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     7861 ttatatatct tgtggaaagg acgaaacacc ggagacggtt gtaaatgagc acacaaaata
     7921 cacatgctaa aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa
     7981 taactttagt atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat
     8041 gagcaaaata aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat
     8101 agctactcca ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc
     8161 ttcaaatgtt ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt
     8221 cattagttta acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac
     8281 aaattctaat ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa
     8341 taaacaattt tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt
     8401 catgtcttct aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat
     8461 atcaatttct gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt
     8521 tgtgcatgca cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa
     8581 taattcttta caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc
     8641 aattgcttca tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt
     8701 gtgatctata tatttgttta gtttcatttt ttctcctata tattcatttt taattttaat
     8761 tctttaataa tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata
     8821 aaataaattt ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct
     8881 tctaaataaa attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct
     8941 tcaacattaa caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc
     9001 attaatggtt caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa
     9061 gtgtaatatg cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt
     9121 tgttttttat aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta
     9181 tctctagctg agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt
     9241 aatccgttat cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt
     9301 tttacaatat taactccata tttttcatga gcgattctat cacccaaatc acttgttaca
     9361 aaacttgaat atagagccgg attttttgga atgctattta agcgttttag atttgataat
     9421 tttcaatcaa ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca
     9481 tgttttattg ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca
     9541 tcatgtttaa taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga
     9601 tttacaacat ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta
     9661 tatcctgatt cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta
     9721 aaaataattt ttaaatcttt tttagcttct tgctcttttt tgtacgtctc tgttttagag
     9781 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     9841 tcggtgcttt tttaagcttg gcgtaactag atcttgagac aaatggcagt attcatccac
     9901 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9961 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    10021 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg gg
//
