LOCUS       sequence-255156-       11646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: pHWBF09exp
FEATURES             Location/Qualifiers
     source          1..11646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2189..2213)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2585..2764
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2800..3825
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4023..4275
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4368..4392
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4913..5704
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5950..6538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6439..6458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6692..6709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6724..6864
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6790..6809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6950..6972
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7208..7402
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7468..8541)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8970..9599)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10930..10954
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccgatcctgg tggagctgga cggggatgtt
      541 aacggccaca agttctcagt gtccggcgag ggggagggcg acgccaccta cgggaagctc
      601 acgctgaagt tcatttgcac gaccggcaag ctgccggtgc cctggccaac cctggtcaca
      661 actctgacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaagt ccgctatgcc agagggctac gtgcaggagc gcacgatctt cttcaaggac
      781 gatggcaact acaagaccag ggccgaggtc aagttcgagg gggacacgct cgttaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata tcctcggcca caagctggag
      901 tacaactaca attcgcataa cgtctacatc atggcggaca agcagaagaa cgggattaag
      961 gttaatttca agatccggca caatattgag gacggcagcg tgcagctcgc tgatcattac
     1021 cagcagaaca caccgatcgg ggacggccct gtcctcctgc ccgataatca ctacctctct
     1081 actcagtcag cgctgtccaa ggacccgaac gagaagcgcg atcatatggt cctcctggag
     1141 ttcgttacag ccgcggggat cactctcggc atggacgagc tgtacaaggg ggctggcgat
     1201 tacaaggacg atgacgataa ggcctacccc tacgacgtgc cagattacgc cggggctggc
     1261 atgagggcgg gcgacgcgtt cagcaactac cgctgcagga ccatgtcgat gaattacgac
     1321 ggcgatagga gcgaggatcg gcagtcggag gaggtgcagt ctgcctacaa gcgggacagc
     1381 gatgtcggcg aggagatgat catttccagc gaggagcaga acgacgagaa tgagtggagc
     1441 tgggtcaacc aggaccggaa tggcgatccc ttcgcggagt cggtttcgtc tctccacacc
     1501 acgcagcaga tcctggagaa tgagattcgc aagctgtcgg agctggggaa ggagctggag
     1561 gcggaggaga gcacctcggg caacaaggac caggatgtta tcctcctgcc atactctgag
     1621 gccgacgtgc tggagctgtc agagaagatg gagcacctgg agcagaaggt caaggaggcg
     1681 tcgaacacca ttcgcgagaa ggacctcatg ctgtctaagc tgcagatcct cattggcacg
     1741 gcggatcggg ctgccctgga ggaggagctg gaggaggccg ctgcttccat cgaccagctg
     1801 gagacggagc tggagcgcca cctccaggag aagctggagg cggagattca gtgcctcctg
     1861 atgctcaagg cttcccagaa ctggcaggtt cgcgcggagg acagggtggc cctggaggag
     1921 catagggctc tgtctgccgg cgataagaca cggatgctcc tgaagctgcg cgagactgag
     1981 agcaagatcg tcatgctcaa ggagcaggtg gacaagctgg aggtcaggga gaaggagctg
     2041 aaccggacaa ctgaggtgct gaagatgcag tcacggacat tccgcgcgtc cctgttcggc
     2101 ctcgttcagc tcgtgatgct ctgcctgtcc ctcaaggttt tcttcgcgcg cgtgagcgct
     2161 cctttcgacg aggccgtgcc gacttgagac ccagctttct tgtacaaagt ggtgatatcc
     2221 cgcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
     2281 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
     2341 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     2401 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcga
     2461 cgtcgggccc tctagaggat ccccgggtac cgcgaattat cgatcatgag cggagaatta
     2521 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact
     2581 gacagaaccg caacgttgaa ggagccactg agccgcgggt ttctggagtt taatgagcta
     2641 agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct
     2701 agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca
     2761 attagagtct catattcact ctcaactcga atccccccta tgaaaaagcc tgaactcacc
     2821 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     2881 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     2941 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     3001 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagttcag cgagagcctg
     3061 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     3121 ctgcccgctg ttcttcagcc ggtcgcggag gctatggatg ctatcgctgc ggccgatctt
     3181 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     3241 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     3301 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     3361 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     3421 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     3481 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     3541 tacttcgagc ggaggcatcc ggagcttgca ggatcgccaa ggctccgggc gtatatgctc
     3601 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     3661 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     3721 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     3781 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     3841 aacaagagct gatttcgaga acgcctcagc cagcaactcg cgcgagccta gcaaggcaaa
     3901 tgcgagagaa cggccttacg cttggtggca cagttctcgt ccacagttcg ctaagctcgc
     3961 tcggctgggt cgcgggaggg ccggtcgcag tgattcagga attaattccc tagagtcaag
     4021 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     4081 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     4141 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     4201 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     4261 ctatgttact agatcgaccg gcatgcaagc tgataattca attcggcgtt aattcagtac
     4321 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     4381 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     4441 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     4501 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     4561 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     4621 cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     4681 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     4741 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     4801 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     4861 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga
     4921 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     4981 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     5041 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     5101 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     5161 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     5221 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     5281 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     5341 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     5401 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     5461 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     5521 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     5581 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     5641 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     5701 ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     5761 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     5821 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac
     5881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6061 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6601 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6661 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6721 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     6781 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     6841 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     6901 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6961 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     7021 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     7081 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     7141 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     7201 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     7261 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     7321 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     7381 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
     7441 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     7501 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     7561 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     7621 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     7681 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     7741 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     7801 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     7861 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     7921 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     7981 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     8041 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     8101 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     8161 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     8221 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     8281 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     8341 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     8401 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     8461 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     8521 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     8581 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     8641 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     8701 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     8761 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     8821 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     8881 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     8941 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     9001 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     9061 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     9121 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     9181 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     9241 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     9301 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     9361 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     9421 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     9481 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     9541 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     9601 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     9661 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
     9721 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
     9781 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
     9841 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
     9901 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
     9961 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    10021 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    10081 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    10141 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    10201 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    10261 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    10321 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    10381 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    10441 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    10501 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    10561 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    10621 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    10681 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    10741 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa
    10801 gtactttaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
    10861 acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt
    10921 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg
    10981 ggaaacgaca atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag
    11041 agccaagctg atctcctttg ccccggagat caccatggac gactttctct atctctacga
    11101 tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa
    11161 gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc
    11221 ggcaggtctc atcaagacga tctacccgag taataatctc caggagatca aataccttcc
    11281 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
    11341 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
    11401 taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa
    11461 ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga
    11521 acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
    11581 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
    11641 ggctat
//
