LOCUS       sequence-255158-        8129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector for the expression of constitutive Stat5a 
            (hygromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Mallette FA, Gaumont-Leclerc MF, Huot G, Ferbeyre G
  TITLE     Myc down-regulation as a mechanism to activate the Rb pathway in 
            STAT5A-induced senescence.
  JOURNAL   J Biol Chem. 2007 Nov 30;282(48):34938-44. doi: 
            10.1074/jbc.M707074200. Epub 2007 Oct 3.
  PUBMED    17913706
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M707074200"; journalName: "J Biol Chem"; date: 
            "2007-11-30- 30"; volume: "282"; issue: "48"; pages: "34938-44"
COMMENT     Sequence Label: pWZL-Stat5a1*6
FEATURES             Location/Qualifiers
     source          1..8129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3936..4397
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3992..4009)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4219..4238
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4402..5427
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4402..5427
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             5616..6085
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6215..6232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6386..6974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6466..6485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7134..7925,7926..7994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7757..7776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7995..8099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcggcac gagaccgccg ccgccgtcaa gagccgtcag gagccgtcag aagccccggc
     1441 ctggagcgac agccgcaggc gctccgcagc accaggtaaa cagccatggc gggctggatt
     1501 caggcccagc agcttcaggg agatgccctg cgccagatgc aagtgttgta tgggcagcat
     1561 ttccccatcg aggtccggca ctacctggcc cagtggatcg agagccagcc gtgggatgct
     1621 attgacttgg ataatcccca ggaccgaggt caggccaccc aactcctgga gggcctggtg
     1681 caggagctgc agaagaaggc ggagcaccag gtgggggaag atgggttttt gctgaagatc
     1741 aagctggggc actatgccac acagctccag aacacgtatg accgctgtcc catggagctg
     1801 gttcgctgta tccgtcacat tctgtacaac gaacagaggc tggttcgcga agccaacaat
     1861 tgcagctccc ctgctggtgt cctggttgac gccatgtccc agaagcacct tcagatcaac
     1921 caaaggtttg aggagctgcg cctgatcaca caggacacgg agaacgagct gaagaagctg
     1981 cagcagaccc aagagtactt catcatccag taccaggaga gcctgcggat ccaagctcag
     2041 tttgcccagc tgggacagct gaacccccag gagcgcatga gcagggagac ggccctccag
     2101 cagaagcaag tgtccctgga gacctggctg cagcgagagg cacagacact gcagcagtac
     2161 cgagtggagc tggctgagaa gcaccagaag accctgcagc tgctgcggaa gcagcagacc
     2221 atcatcctgg acgacgagct gatccagtgg aagcggagac agcagctggc cgggaacggg
     2281 ggtccccccg agggcagcct ggacgtgctg cagtcctggt gtgagaagct ggccgagatc
     2341 atctggcaga accggcagca gatccgcagg gctgagcgcc tgtgccagca gctgcccatc
     2401 ccaggccccg tggaggagat gctggctgag gtcaacgcca ccatcacgga catcatctca
     2461 gctctggtca ccagcacgtt catcatcgag aagcagcctc ctcaggtcct gaagacccag
     2521 accaagtttg cggccaccgt gcgcctgctg gtggggggaa agctgaatgt gcacatgaac
     2581 cccccgcagg tgaaggcgac catcatcagc gagcagcagg ccaagtccct gctcaagaat
     2641 gagaacaccc gcaatgagtg cagcggcgag atcctgaaca actgttgcgt catggagtac
     2701 caccaggcca ctggcacgct cagcgcccac ttcagaaaca tgtcactgaa aagaatcaag
     2761 cgcgccgaca ggcgtggtgc agagtcggtg acggaggaga agttcacagt cctgtttgag
     2821 tctcagttca gcgttggcag caacgagctg gtgttccagg tgaagaccct gtccctccct
     2881 gtggtcgtta tcgtccatgg cagccaggac cacaatgcta ctgccaccgt gctgtgggac
     2941 aatgcctttg ctgagccggg cagggtgcca tttgctgtgc ctgacaaggt gctgtggccg
     3001 cagctgtgtg aagcgctcaa catgaaattc aaggctgaag tacagagcaa ccggggcttg
     3061 accaaggaga acctcgtgtt cctggcacag aaactgttca acatcagcag caaccacctc
     3121 gaggactaca acagcatgtc tgtgtcctgg tcccagttca accgggagaa cttgcccggc
     3181 tggaactaca ccttctggca gtggttcgac ggggtgatgg aggtgctgaa gaagcaccat
     3241 aagccccatt ggaatgatgg ggctatcctg ggtttcgtga acaagcaaca ggcccacgac
     3301 ctgctcatca acaagccgga cgggaccttc ctgctgcgct tcagtgactc ggaaatcggg
     3361 ggcatcacca ttgcttggaa gtttgactct ccggaccgaa acctctggaa tctgaagcca
     3421 ttcacgacgc gagatttctc cattcggtcc ctggccgacc ggctggggga cctgaactac
     3481 cttatctacg tgttcccaga ccgacccaag gacgaggtct ttgccaagta ttacactcct
     3541 gtacttgcga aagcagttga cggatacgtg aagccacaga tcaagcaagt ggtccctgag
     3601 ttcgtcaatg cattcacaga tgccggagcc agcgccacct acatggacca ggctccttcc
     3661 ccagtcgtgt gccctcaacc tcactacaac atgtacccac ccaaccctga ccctgtcctt
     3721 gaccaagatg gcgagtttga cctggatgag agcatggatg ttgccaggca cgtggaagaa
     3781 cttttacgcc ggcccatgga cagtctcgac gcccgcctct ccccacctgc tggtctcttc
     3841 acctccgcta gaagctccct gtcctgagcg gccgccagca cagtggtcga ccaattccgg
     3901 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     3961 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     4021 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     4081 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     4141 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     4201 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4261 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4321 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4381 gaaaaacacg atgataatac catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     4441 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     4501 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     4561 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     4621 attccggaag tgcttgacat tggggaattt agcgagagcc tgacctattg catctcccgc
     4681 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     4741 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     4801 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     4861 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     4921 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     4981 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     5041 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     5101 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     5161 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     5221 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     5281 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     5341 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     5401 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgggatctat cgataaaata
     5461 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     5521 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     5581 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     5641 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     5701 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     5761 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     5821 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     5881 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     5941 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     6001 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     6061 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     6121 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
     6181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7081 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8101 acatttcccc gaaaagtgcc acctgcagc
//
