LOCUS       sequence-255167-        7574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector for the expression of constitutive Stat5a 
            (puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Mallette FA, Gaumont-Leclerc MF, Huot G, Ferbeyre G
  TITLE     Myc down-regulation as a mechanism to activate the Rb pathway in 
            STAT5A-induced senescence.
  JOURNAL   J Biol Chem. 2007 Nov 30;282(48):34938-44. doi: 
            10.1074/jbc.M707074200. Epub 2007 Oct 3.
  PUBMED    17913706
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M707074200"; journalName: "J Biol Chem"; date: 
            "2007-11-30- 30"; volume: "282"; issue: "48"; pages: "34938-44"
COMMENT     Sequence Label: pBABE-Stat5a1*6
FEATURES             Location/Qualifiers
     source          1..7574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3897..3917)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3902..4231
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4082..4217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4241..4840
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4241..4260)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4737..4757
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5061..5530
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5660..5677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5831..6419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5911..5930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6579..7370,7371..7439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7202..7221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7440..7544)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcggcac gagaccgccg ccgccgtcaa gagccgtcag gagccgtcag aagccccggc
     1441 ctggagcgac agccgcaggc gctccgcagc accaggtaaa cagccatggc gggctggatt
     1501 caggcccagc agcttcaggg agatgccctg cgccagatgc aagtgttgta tgggcagcat
     1561 ttccccatcg aggtccggca ctacctggcc cagtggatcg agagccagcc gtgggatgct
     1621 attgacttgg ataatcccca ggaccgaggt caggccaccc aactcctgga gggcctggtg
     1681 caggagctgc agaagaaggc ggagcaccag gtgggggaag atgggttttt gctgaagatc
     1741 aagctggggc actatgccac acagctccag aacacgtatg accgctgtcc catggagctg
     1801 gttcgctgta tccgtcacat tctgtacaac gaacagaggc tggttcgcga agccaacaat
     1861 tgcagctccc ctgctggtgt cctggttgac gccatgtccc agaagcacct tcagatcaac
     1921 caaaggtttg aggagctgcg cctgatcaca caggacacgg agaacgagct gaagaagctg
     1981 cagcagaccc aagagtactt catcatccag taccaggaga gcctgcggat ccaagctcag
     2041 tttgcccagc tgggacagct gaacccccag gagcgcatga gcagggagac ggccctccag
     2101 cagaagcaag tgtccctgga gacctggctg cagcgagagg cacagacact gcagcagtac
     2161 cgagtggagc tggctgagaa gcaccagaag accctgcagc tgctgcggaa gcagcagacc
     2221 atcatcctgg acgacgagct gatccagtgg aagcggagac agcagctggc cgggaacggg
     2281 ggtccccccg agggcagcct ggacgtgctg cagtcctggt gtgagaagct ggccgagatc
     2341 atctggcaga accggcagca gatccgcagg gctgagcgcc tgtgccagca gctgcccatc
     2401 ccaggccccg tggaggagat gctggctgag gtcaacgcca ccatcacgga catcatctca
     2461 gctctggtca ccagcacgtt catcatcgag aagcagcctc ctcaggtcct gaagacccag
     2521 accaagtttg cggccaccgt gcgcctgctg gtggggggaa agctgaatgt gcacatgaac
     2581 cccccgcagg tgaaggcgac catcatcagc gagcagcagg ccaagtccct gctcaagaat
     2641 gagaacaccc gcaatgagtg cagcggcgag atcctgaaca actgttgcgt catggagtac
     2701 caccaggcca ctggcacgct cagcgcccac ttcagaaaca tgtcactgaa aagaatcaag
     2761 cgcgccgaca ggcgtggtgc agagtcggtg acggaggaga agttcacagt cctgtttgag
     2821 tctcagttca gcgttggcag caacgagctg gtgttccagg tgaagaccct gtccctccct
     2881 gtggtcgtta tcgtccatgg cagccaggac cacaatgcta ctgccaccgt gctgtgggac
     2941 aatgcctttg ctgagccggg cagggtgcca tttgctgtgc ctgacaaggt gctgtggccg
     3001 cagctgtgtg aagcgctcaa catgaaattc aaggctgaag tacagagcaa ccggggcttg
     3061 accaaggaga acctcgtgtt cctggcacag aaactgttca acatcagcag caaccacctc
     3121 gaggactaca acagcatgtc tgtgtcctgg tcccagttca accgggagaa cttgcccggc
     3181 tggaactaca ccttctggca gtggttcgac ggggtgatgg aggtgctgaa gaagcaccat
     3241 aagccccatt ggaatgatgg ggctatcctg ggtttcgtga acaagcaaca ggcccacgac
     3301 ctgctcatca acaagccgga cgggaccttc ctgctgcgct tcagtgactc ggaaatcggg
     3361 ggcatcacca ttgcttggaa gtttgactct ccggaccgaa acctctggaa tctgaagcca
     3421 ttcacgacgc gagatttctc cattcggtcc ctggccgacc ggctggggga cctgaactac
     3481 cttatctacg tgttcccaga ccgacccaag gacgaggtct ttgccaagta ttacactcct
     3541 gtacttgcga aagcagttga cggatacgtg aagccacaga tcaagcaagt ggtccctgag
     3601 ttcgtcaatg cattcacaga tgccggagcc agcgccacct acatggacca ggctccttcc
     3661 ccagtcgtgt gccctcaacc tcactacaac atgtacccac ccaaccctga ccctgtcctt
     3721 gaccaagatg gcgagtttga cctggatgag agcatggatg ttgccaggca cgtggaagaa
     3781 cttttacgcc ggcccatgga cagtctcgac gcccgcctct ccccacctgc tggtctcttc
     3841 acctccgcta gaagctccct gtcctgagcg gccgccagca cagtggtcga ccctgtggaa
     3901 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3961 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4021 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4081 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4141 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4201 aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt acaagcccac
     4261 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4321 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4381 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4441 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4501 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4561 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4621 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4681 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4741 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4801 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     4861 gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga ttttatttag
     4921 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     4981 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     5041 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     5101 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     5161 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     5221 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     5281 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     5341 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     5401 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     5461 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     5521 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     5581 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5641 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6241 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6481 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccgcaaa
     6541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7561 gtgccacctg cagc
//
