LOCUS       sequence-255175-       12070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Codon Optimized dCas9-DNMT3A3L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Tarjan DR, Flavahan WA, Bernstein BE
  TITLE     Epigenome editing strategies for the functional annotation of CTCF 
            insulators.
  JOURNAL   Nat Commun. 2019 Sep 18;10(1):4258. doi: 10.1038/s41467-019-12166-w.
  PUBMED    31534142
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-18"; volume: "10(1):4258. doi"; pages: " 
            10.1038/s41467-019-12166-w"
COMMENT     Sequence Label: pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
FEATURES             Location/Qualifiers
     source          1..12070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(763..789,790..792,793..3276,3277..3282,3283..3348,
                     3349..3351,3352..4866)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             4323..4331
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4879..4899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6592..6612
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6628..6684
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6685..6687,6688..7401)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6685..7398
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6727..6748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6988..7007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7335..7356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7427..7444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7433..7657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7703..8131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7790..7809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8000..8021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8140..8160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8145..8474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8325..8460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8387..8406
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8541..9335
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8595..8614)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9205..9224
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9509..9630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9546..9565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9600..9619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9679..9695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9679..9695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9692..9714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9703..9719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9727..9733,9734..9751,9752..9757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9772..9793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9910..9927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10081..10669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10161..10180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10840..11631,11632..11700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11463..11482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11701..11805)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11880..11899)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gaatggacaa gaagtattca
      781 atcggcctgg ctatcggaac caacagcgtg gggtgggcag tcatcacaga cgagtataag
      841 gtcccctcca agaagtttaa ggtgctgggc aataccgatc gccactccat caagaagaac
      901 ctgatcggcg ccctgctgtt cgactctggc gagacagcag aggcaaccag gctgaagagg
      961 acagcacgga gaaggtatac ccgccggaag aaccggatct gctacctgca ggagatcttc
     1021 agcaatgaga tggccaaggt ggacgattcc ttctttcacc gcctggagga gtctttcctg
     1081 gtggaggagg ataagaagca cgagcggcac ccaatctttg gcaacatcgt ggacgaggtg
     1141 gcctatcacg agaagtaccc caccatctat cacctgcgga agaagctggt ggattccaca
     1201 gacaaggccg atctgagact gatctatctg gccctggccc acatgatcaa gttcaggggc
     1261 cactttctga tcgagggcga cctgaatccc gataacagcg atgtggacaa gctgttcatc
     1321 cagctggtgc agacctacaa ccagctgttt gaggagaatc ctatcaacgc ctctggagtg
     1381 gacgcaaagg caatcctgag cgccagactg tctaagagca gaaggctgga gaatctgatc
     1441 gcccagctgc ctggcgagaa gaagaatggc ctgtttggca acctgatcgc cctgtccctg
     1501 ggcctgaccc caaatttcaa gtctaacttt gacctggccg aggatgccaa gctgcagctg
     1561 tccaaggata catatgacga tgacctggac aacctgctgg cccagatcgg cgaccagtac
     1621 gccgatctgt tcctggccgc caagaacctg agcgatgcca tcctgctgtc cgacatcctg
     1681 cgcgtgaata cagagatcac caaggcccct ctgtccgcct ctatgatcaa gagatatgat
     1741 gagcaccacc aggacctgac cctgctgaag gccctggtga ggcagcagct gccagagaag
     1801 tacaaggaga tcttctttga tcagagcaag aacggatacg caggatatat cgacggagga
     1861 gcatcccagg aggagttcta caagtttatc aagcctatcc tggagaagat ggacggcacc
     1921 gaggagctgc tggtgaagct gaaccgggag gacctgctgc ggaagcagag aacattcgat
     1981 aatggcagca tcccccacca gatccacctg ggagagctgc acgcaatcct gcgccggcag
     2041 gaggatttct acccttttct gaaggacaat agggagaaga tcgagaagat cctgaccttc
     2101 cgcatcccat actatgtggg accactggca aggggaaaca gcagatttgc ctggatgaca
     2161 agaaagtccg aggagacaat caccccttgg aatttcgagg aggtggtgga taagggcgcc
     2221 tctgcccaga gcttcatcga gaggatgacc aactttgaca agaatctgcc caacgagaag
     2281 gtgctgccta agcactctct gctgtacgag tatttcacag tgtataatga gctgaccaag
     2341 gtgaagtacg tgacagaggg catgagaaag ccagccttcc tgagcggcga gcagaagaag
     2401 gccatcgtgg acctgctgtt taagacaaac aggaaggtga ccgtgaagca gctgaaggag
     2461 gactatttca agaagatcga gtgttttgat tccgtggaga tctctggcgt ggaggaccgg
     2521 tttaatgcct ccctgggcac ataccacgat ctgctgaaga tcatcaagga taaggacttc
     2581 ctggacaatg aggagaacga ggacatcctg gaggatatcg tgctgacact gaccctgttt
     2641 gaggatcggg agatgatcga ggagagactg aagacctatg cccacctgtt cgatgacaaa
     2701 gtgatgaagc agctgaagag aaggcgctac acaggatggg gcaggctgag ccgcaagctg
     2761 atcaacggca tcagagacaa gcagtctggc aagaccatcc tggattttct gaagagcgac
     2821 ggcttcgcca acaggaactt catgcagctg atccacgatg actccctgac attcaaggag
     2881 gatatccaga aggcacaggt gtccggacag ggcgactctc tgcacgagca catcgccaat
     2941 ctggccggca gcccagccat caagaagggc atcctgcaga ccgtgaaggt ggtggacgag
     3001 ctggtgaaag tgatgggcag gcacaagccc gagaatatcg tgatcgagat ggcccgcgag
     3061 aaccagacca cacagaaggg ccagaagaat agcagggagc gcatgaagcg catcgaggag
     3121 ggcatcaagg agctgggctc ccagatcctg aaggagcacc ccgtggagaa tacccagctg
     3181 cagaacgaga agctgtatct gtactatctg cagaacggcc gggacatgta cgtggatcag
     3241 gagctggaca tcaatagact gtctgattat gacgtggccg ccatcgtgcc ccagtctttc
     3301 ctgaaggatg acagcatcga caacaaggtg ctgacacgga gcgataaggc cagaggcaag
     3361 agcgacaatg tgccttccga ggaggtggtg aagaagatga agaattactg gcggcagctg
     3421 ctgaacgcca agctgatcac ccagcggaag tttgataacc tgacaaaggc agagagagga
     3481 ggcctgtccg agctggacaa ggcaggcttc atcaagaggc agctggtgga gacacgccag
     3541 atcaccaagc acgtggccca gatcctggat tctcgcatga ataccaagta cgacgagaac
     3601 gataagctga tccgggaggt gaaagtgatc accctgaaga gcaagctggt gtccgacttc
     3661 cggaaggact tccagtttta taaggtgcgc gagatcaaca attatcacca cgcccacgac
     3721 gcatacctga acgcagtggt gggaaccgcc ctgatcaaga agtacccaaa gctggagagc
     3781 gagttcgtgt acggcgatta taaggtgtac gacgtgagaa agatgatcgc caagtccgag
     3841 caggagatcg gcaaggccac agccaagtat ttcttttact ctaacatcat gaacttcttt
     3901 aagacagaga tcaccctggc aaacggagag atcaggaagc ggcccctgat cgagacaaat
     3961 ggcgagacag gcgagatcgt gtgggataag ggccgggact ttgccaccgt gagaaaggtg
     4021 ctgagcatgc cacaggtgaa catcgtgaag aagacagagg tgcagaccgg cggcttctcc
     4081 aaggagtcta tcctgcccaa gaggaattcc gataagctga tcgcccgcaa gaaggattgg
     4141 gaccccaaga agtatggcgg cttcgactcc cctaccgtgg cctactctgt gctggtggtg
     4201 gccaaggtgg agaagggcaa gtctaagaag ctgaagagcg tgaaggagct gctgggcatc
     4261 acaatcatgg agaggagctc cttcgagaag aaccccatcg attttctgga ggccaagggc
     4321 tataaggagg tgaagaagga cctgatcatc aagctgccta agtactccct gtttgagctg
     4381 gagaatggaa ggaagaggat gctggcatct gccggagagc tgcagaaggg caacgagctg
     4441 gccctgccaa gcaagtacgt gaatttcctg tatctggcca gccactacga gaagctgaag
     4501 ggctcccccg aggataacga gcagaagcag ctgtttgtgg agcagcacaa gcactatctg
     4561 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgatgccaac
     4621 ctggacaagg tgctgtccgc ctacaataag cacagggaca agcctatccg cgagcaggcc
     4681 gagaacatca tccacctgtt cacactgacc aatctgggcg ccccagccgc cttcaagtat
     4741 tttgatacca caatcgaccg gaagagatac acatctacca aggaggtgct ggatgccacc
     4801 ctgatccacc agagcatcac aggcctgtac gagacaagga tcgacctgag ccagctggga
     4861 ggcgattccc gggcagatcc caagaagaag cggaaggtgg aggcaagcgg atccggaagg
     4921 gcatctcctg gaatcccagg aagcacccgc aaccacgatc aggagtttga cccccctaag
     4981 gtgtacccac ccgtgccagc cgagaagagg aagcccatcc gcgtgctgtc cctgttcgac
     5041 ggcatcgcca caggcctgct ggtgctgaag gatctgggca tccaggtgga cagatatatc
     5101 gcctccgagg tgtgcgagga ttctatcacc gtgggcatgg tgaggcacca gggcaagatc
     5161 atgtacgtgg gcgacgtgcg cagcgtgaca cagaagcaca tccaggagtg gggacccttc
     5221 gacctggtca tcggaggcag cccctgtaat gacctgtcca tcgtgaaccc tgcaaggaag
     5281 ggcctgtatg agggaaccgg cagactgttc tttgagttct acaggctgct gcacgacgcc
     5341 cgccctaagg agggcgatga caggccattc ttttggctgt ttgagaacgt ggtggccatg
     5401 ggcgtgagcg acaagcggga tatctccaga ttcctggagt ctaatcccgt gatgatcgat
     5461 gcaaaggagg tgtctgccgc acacagggca aggtactttt ggggaaatct gcctggcatg
     5521 aaccgcccac tggccagcac cgtgaacgac aagctggagc tgcaggagtg cctggagcac
     5581 ggaaggatcg ccaagttctc caaggtgcgg acaatcacca caagatctaa cagcatcaag
     5641 cagggcaagg atcagcactt ccccgtgttc atgaatgaga aggaggacat cctgtggtgt
     5701 accgagatgg agcgcgtgtt cggctttcca gtgcactata cagacgtgag caatatgagc
     5761 cggctggcaa ggcagagact gctgggccgg tcctggtctg tgccagtgat cagacacctg
     5821 ttcgcccccc tgaaggagta ctttgcctgc gtgtctagcg gcaactctaa tgccaacagc
     5881 agaggccctt ccttttcctc tggcctggtg ccactgtctc tgaggggcag ccacatgggc
     5941 cccatggaga tctacaagac cgtgtccgcc tggaagaggc agcctgtgcg cgtgctgtct
     6001 ctgttccgca acatcgacaa ggtgctgaag agcctgggct ttctggagag cggatccgga
     6061 tctggaggag gcaccctgaa gtatgtggag gatgtgacaa atgtggtgcg gagagacgtg
     6121 gagaagtggg gccccttcga tctggtgtac ggatccaccc agccactggg aagctcctgc
     6181 gataggtgtc caggatggta tatgttccag tttcacagaa tcctgcagta cgcactgcca
     6241 aggcaggaga gccagcgccc tttcttttgg atctttatgg acaacctgct gctgacagag
     6301 gatgaccagg agacaacaac ccgcttcctg cagacagagg cagtgaccct gcaggatgtg
     6361 aggggacgcg actatcagaa tgccatgcgg gtgtggtcta acatccctgg cctgaagagc
     6421 aagcacgccc ccctgacccc taaggaggag gagtacctgc aggcccaggt gcggagcaga
     6481 tccaagctgg atgcccctaa ggtggacctg ctggtgaaga attgtctgct gccactgcgg
     6541 gagtacttca agtactttag tcagaatagc ctgccactgt cacgagccga ccccaagaag
     6601 aaacggaagg tgtctagagg aagcggagct actaacttca gcctgctgaa gcaggctgga
     6661 gacgtggagg agaaccctgg acctatgagc aagggcgagg agctgttcac cggggtggtg
     6721 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6781 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6841 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     6901 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6961 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     7021 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     7081 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     7141 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     7201 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     7261 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     7321 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc
     7381 atggacgagc tgtacaagta agggcccgtt taaacccgct gatcagcctc gactgtgcct
     7441 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7501 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7561 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7621 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7681 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7741 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8041 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8101 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     8161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8221 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8281 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8341 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8401 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8461 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     8521 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     8581 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     8641 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     8701 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     8761 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     8821 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     8881 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     8941 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     9001 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     9061 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     9121 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     9181 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     9241 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     9301 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     9361 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     9421 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     9481 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     9541 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9601 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9661 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9721 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9781 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9841 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9901 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11101 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11161 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11221 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11281 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11341 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11401 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11461 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11521 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11581 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11641 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11701 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11761 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11821 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
    11881 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
    11941 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
    12001 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
    12061 tatacgcgtt
//
