LOCUS       sequence-255177-        2673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus right homology arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I2R
FEATURES             Location/Qualifiers
     source          1..2673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1038..1072)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1143..1802)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1717..1736
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1984..2572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2473..2492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agttgaagca tagaagaaat gtttaaccaa aaactatcct ttttatttat
       61 tttgaatttt ctctctcata tttttgtaaa acgatacttt aaccacactc aagcttttgc
      121 ttgctactca aatacctgaa atctttatag acaattttcc ttcttattcg taataataaa
      181 actatattgt tctcttcctg tttttttttt taaaaaaaaa aactgtcaaa atctccccca
      241 cagaaaaata aaaacaaatt atacttttgg gagtaaacca tgaacttaaa acaacagttc
      301 atattcttaa gtagtgccac tatagttaac cagagacaat ttaactccta aatcttcata
      361 ctgaagattg acataaacta aataatgact acactgttac ttttgagtca gttgggtctt
      421 ttggcattcg ccaatgctat taaccctatg gtaaagggcg aatatagaat tgaacatcct
      481 aagataggag acttgtcaga aattgaatct gctatctctc ccaaatcttt ctatgaatca
      541 tttcaatatg gttccgaagc ggagttccgt aagaattggg tttcttctga tctaacaatg
      601 gaacatgtag acgaaacatc ttcgaaggcg ttccctggca catggaagtt gcagtcacca
      661 tttttggtcc caggattcag tgatgatcta tctttagttt tgggtagcga acaaactaga
      721 gctgctattg cccataggtt aaaatacgat ctatcgatca aggataatga gaaactagtg
      781 gtgcaatatg aagttaaact tcaaaaaatg cttgaatgtg gcggtgcata tatcaaattg
      841 ttgaaaacct ccgcaactga tctttctcag tacgaccact cgtcagatga ctatgctgtc
      901 atctttggcc cagacttttg ccaaggttat gtggacgaaa ttcatttggt tttgaagcgc
      961 gagaatcctt tttctggaaa atctgaagac aaattcttat taaatgccgg cgcgcctcag
     1021 ccgatgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1081 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1141 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     1201 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1261 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1321 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     1381 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1441 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1501 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1561 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1621 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1681 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1741 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1801 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1861 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1921 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1981 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2041 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2101 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2161 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2221 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2281 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2341 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2401 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2461 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2521 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2581 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2641 tgcgttatcc cctgattctg tggataaccg tag
//
