LOCUS       sequence-25519-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Chen G, DenBoer L, Shin J
  TITLE     Design of a single plasmid-based modified yeast one-hybrid system 
            for investigation of in vivo protein-protein and protein-DNA 
            interactions.
  JOURNAL   Biotechniques. 2008 Sep . 45(3):295-304.
  PUBMED    18778253
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2008 Sep . 45(3):295-304."
COMMENT     Sequence Label: pCETT
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(198..302)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(345..532)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     453..477
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cattggaacg ttcttcgggg cgaaaactct caaggatctt accgctgttt gagatccagt
       61 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      301 cgcacatttc cccgaaaagt gccacctgac gtcaagcttg catgccggta gaggtgtggt
      361 caataagagc gacctcatgc tatacctgag aaagcaacct gacctacagg aaagagttac
      421 tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta
      481 ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag
      541 aagtgtcaac aacgtatcta ccaacgattt gacccttttc catcttttcg taaatttctg
      601 gcaaggtaga caagccgaca accttgattg gagacttgac caaacctctg gcgaagaagt
      661 ccaaagcttc tgaatacncc cccgtantna ttttnccnga cnggtttggg nnccaaanaa
      721 aagatgggcc ttaattctag ccattaaaaa ttctatagat canaggtcnc cggcccncaa
      781 tgnanctttn aggggttntt ttcntnaaaa ag
//
