LOCUS       sequence-255191-       11512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: GFP-PK1Dexp
FEATURES             Location/Qualifiers
     source          1..11512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2055..2079)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2451..2630
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2666..3691
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3889..4141
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4234..4258
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4779..5570
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5816..6404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6305..6324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6558..6575
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6590..6730
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6656..6675)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6816..6838
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7074..7268
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7334..8407)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8836..9465)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10796..10820
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccaatcctcg tcgagctgga cggggatgtt
      541 aacggccaca agttctccgt gagcggcgag ggggagggcg acgccaccta cgggaagctc
      601 acgctgaagt tcatttgcac gaccggcaag ctgccggtgc cctggccaac cctggtcaca
      661 actctgacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc ggagggctac gttcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagacaag ggcggaggtt aagttcgagg gcgacactct cgtgaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctcggcca caagctggag
      901 tacaactaca attcgcataa cgtctacatc atggctgaca agcagaagaa cggcattaag
      961 gttaatttca agatccgcca caatattgag gacggctccg tgcagctcgc ggatcattac
     1021 cagcagaaca cgccgatcgg ggacggccct gtcctcctgc cggataatca ctacctctcg
     1081 acgcagtctg cgctgtcaaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacag ccgcggggat cactctcggc atggacgagc tgtacaaggg ggcgggcgac
     1201 tacaaggacg atgacgataa ggcttacccc tacgacgtgc cagattacgc tggggccggc
     1261 ggatccatgg acatgccgcc ggcggcgcag ggacggaggg cgatcgccgt ggtggaccgg
     1321 aagcttgacg agctctgcgc ctgcctcgac gacgcgatgt cgtccaggca gcggagcccg
     1381 tacgcggagg agcggctccg cgcggagatc aagtcgaggg ccgacttcct tcggtcgctc
     1441 atggccgccg aggtggactg ccacggcggg acgcgcccgg agcacctggc tgaggccgag
     1501 gcgcgcttcg ccgtcctaga ggccacgttc gatcggtggg cgcggcgcgc agtcgctgca
     1561 cctgaaccag tcgtcgagga gaaggaggag gaggccgact ccttggggtc ctcccggtcc
     1621 acgtgctcct gcaccgagtc gtgccaggag gcgacggagg gggacgccat gcgcgacgcc
     1681 gtggaaacac gcggcaagga ggggacgtcg gatggcatcg ccgaggacaa cgacgccgaa
     1741 tgcgagacgg tggtaatccg cagggaggag gcggcggcgg ttcctgttgc caagaagcgg
     1801 gatgaggcta tgggaacacg cgaggaggtg gcgctggacg ccattgccaa gaagaagggc
     1861 gataccgatc gcgatgccgc ggcggagacg gggaggagga ccgtagacca gcggcgctgg
     1921 tggaggcgga gtgctgcctg gtgcggtgca gccggcgtcg tcgcgttggt cgcggtcggg
     1981 ttggcggtgg agttggtcgc ggtggctgac cacgatgtga atgcttacgt tgttccgacg
     2041 tagcctgcag gcagacccag ctttcttgta caaagtggtg atatcccgcg gccatgctag
     2101 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     2161 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     2221 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     2281 atttctaatt cctaaaacca aaatccagtg acctgcaggc atgcgacgtc gggccctcta
     2341 gaggatcccc gggtaccgcg aattatcgat catgagcgga gaattaaggg agtcacgtta
     2401 tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac
     2461 gttgaaggag ccactgagcc gcgggtttct ggagtttaat gagctaagca catacgtcag
     2521 aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt
     2581 gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata
     2641 ttcactctca actcgaatcc cccctatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     2701 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     2761 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     2821 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     2881 cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc
     2941 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     3001 tcagccggtc gcggaggcta tggatgctat cgctgcggcc gatcttagcc agacgagcgg
     3061 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     3121 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     3181 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     3241 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     3301 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     3361 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     3421 gcatccggag cttgcaggat cgccaaggct ccgggcgtat atgctccgca ttggtcttga
     3481 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     3541 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     3601 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     3661 ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgaaca agagctgatt
     3721 tcgagaacgc ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc
     3781 cttacgcttg gtggcacagt tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg
     3841 ggagggccgg tcgcagtgat tcaggaatta attccctaga gtcaagcaga tcgttcaaac
     3901 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3961 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     4021 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     4081 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     4141 cgaccggcat gcaagctgat aattcaattc ggcgttaatt cagtacatta aaaacgtccg
     4201 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     4261 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     4321 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     4381 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     4441 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc
     4501 gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     4561 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     4621 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     4681 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     4741 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg
     4801 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     4861 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     4921 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     4981 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     5041 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     5101 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     5161 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     5221 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     5281 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     5341 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     5401 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     5461 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     5521 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta
     5581 gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
     5641 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     5701 gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac
     5761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5941 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6481 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6541 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     6601 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     6661 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     6721 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6781 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6841 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
     6901 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt
     6961 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
     7021 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
     7081 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
     7141 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
     7201 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
     7261 tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
     7321 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
     7381 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
     7441 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
     7501 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
     7561 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
     7621 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
     7681 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
     7741 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
     7801 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
     7861 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
     7921 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
     7981 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
     8041 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
     8101 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
     8161 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
     8221 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
     8281 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
     8341 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
     8401 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
     8461 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
     8521 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
     8581 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
     8641 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
     8701 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
     8761 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
     8821 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
     8881 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
     8941 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
     9001 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
     9061 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
     9121 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
     9181 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
     9241 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
     9301 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
     9361 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
     9421 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
     9481 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
     9541 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
     9601 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
     9661 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
     9721 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
     9781 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
     9841 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
     9901 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
     9961 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    10021 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    10081 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    10141 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    10201 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    10261 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    10321 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    10381 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    10441 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    10501 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    10561 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    10621 tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttaaagtac tttaaagtac
    10681 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    10741 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
    10801 cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct
    10861 gatccaagct caagctaagc ttgagctctc ccatatggtc gactagagcc aagctgatct
    10921 cctttgcccc ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt
    10981 tcgacggaga aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca
    11041 atttcagaaa gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctcatca
    11101 agacgatcta cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag
    11161 atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat atatttctca
    11221 agatcagaag tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag
    11281 taatagagat tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa
    11341 aaattcagat cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga
    11401 gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
    11461 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct at
//
