LOCUS       sequence-255195-       11535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: SUN2delSUN
FEATURES             Location/Qualifiers
     source          1..11535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1189..1212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1216..1242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2078..2102)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2474..2653
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2689..3714
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3912..4164
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4257..4281
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4802..5593
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5839..6427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6328..6347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6581..6598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6613..6753
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6679..6698)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6839..6861
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7097..7291
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7357..8430)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8859..9488)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10819..10843
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtgtcc
      481 aagggcgagg aggacaacat ggccatcatt aaggagttca tgcgcttcaa ggtccacatg
      541 gagggctccg ttaatgggca tgagttcgag atcgaggggg agggcgaggg gaggccctac
      601 gagggcacac agactgccaa gctgaaggtg acgaagggcg ggcctctccc cttcgcctgg
      661 gacatcctgt cgcctcagtt catgtacggc tctaaggcct acgtgaagca ccccgctgac
      721 attccagatt acctcaagct gagcttcccg gagggcttca agtgggagcg ggttatgaac
      781 ttcgaggacg gcggggtggt cacggtgacc caggactcca gcctccagga tggcgagttc
      841 atctacaagg tcaagctgcg cggcacaaat ttcccttccg acgggccggt tatgcagaag
      901 aagactatgg gctgggaggc ctcgtctgag cggatgtacc cggaggatgg cgcgctcaag
      961 ggggagatta agcagcgcct caagctgaag gacggtggcc actacgatgc tgaggtgaag
     1021 accacgtaca aggccaagaa gccggtccag ctcccaggcg cctacaacgt taatatcaag
     1081 ctggacatta catcgcacaa cgaggattac actatcgtgg agcagtacga gagggccgag
     1141 ggcaggcatt ctacgggcgg gatggacgag ctgtacaagg gcgccgggga ttacaaggac
     1201 gatgacgata aggcgtaccc ttacgacgtc cccgattacg cgggcgccgg gatggcttct
     1261 ccctcattcg cggccgctgc tacctctccg gcgtcatccc cactcacgct ggacgctatc
     1321 ccactggcct caaggcccgc cccaggcgcc gctgcggccc agcggaagcg ctcggtgttc
     1381 ctcctgaacc accggcctca tccggtctcg cccaccccgc ccctgcagcc aaccgctgcg
     1441 gcctccgctc agcacgccag gaggaagaag acctctcatc cacctaggcc caggtggcag
     1501 accgtgctct ccatcgctgc caagaatgcc gctctcctgg cggccctcct gtacctcggc
     1561 gacctggcct ggaggtggag ccacccgccc ccacctagcc cgcccccaga ccgggctgcc
     1621 ctggagggct acgcggctcg cgtcgatgag gttgaggcca gcctcacacg gactttccgc
     1681 atgatccagg tccagctgga ggcggttgac aggaagattg atggcgaagt gggggcggcg
     1741 cggggcgacc tcctggcgct cctggaggat aagaggctgg ctctggagag gcagctcacg
     1801 cgcctggacg ctagggccgg cgagctgggg gatgcgctcg ctggcctgaa gaggatggag
     1861 ttcctccgga aggacgagtt cgagaagttc tgggatgagg tgaagggctc actgggctcc
     1921 tcctccgagt cggaggtgga cctcgatcag gtccgggccc tggcgcgcga gatcgtcatg
     1981 cgcgagattg agaagcacgc tgcggacggc atcgggcggg tggattacgc tgtggcttcc
     2041 gtcagccccg gcagctcggc tgggtcgcag gcctgagacc cagctttctt gtacaaagtg
     2101 gtgatatccc gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
     2161 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
     2221 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     2281 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca
     2341 ggcatgcgac gtcgggccct ctagaggatc cccgggtacc gcgaattatc gatcatgagc
     2401 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
     2461 tttggaactg acagaaccgc aacgttgaag gagccactga gccgcgggtt tctggagttt
     2521 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
     2581 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
     2641 cggtatccaa ttagagtctc atattcactc tcaactcgaa tcccccctat gaaaaagcct
     2701 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     2761 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     2821 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     2881 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagttcagc
     2941 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     3001 gaaaccgaac tgcccgctgt tcttcagccg gtcgcggagg ctatggatgc tatcgctgcg
     3061 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     3121 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     3181 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     3241 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     3301 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     3361 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     3421 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccaag gctccgggcg
     3481 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     3541 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     3601 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     3661 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga
     3721 tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc gcgagcctag
     3781 caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc cacagttcgc
     3841 taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggaa ttaattccct
     3901 agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     3961 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4021 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4081 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4141 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     4201 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     4261 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     4321 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     4381 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     4441 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     4501 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     4561 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     4621 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     4681 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     4741 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     4801 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     4861 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     4921 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     4981 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5041 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5101 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     5161 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     5221 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     5281 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     5341 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     5401 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     5461 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     5521 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     5581 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     5641 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     5701 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     5761 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5941 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6481 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6541 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6601 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6661 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     6721 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     6781 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     6841 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     6901 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     6961 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     7021 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     7081 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     7141 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     7201 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     7261 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     7321 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     7381 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     7441 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     7501 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     7561 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     7621 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     7681 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     7741 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     7801 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     7861 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     7921 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     7981 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     8041 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     8101 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     8161 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     8221 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     8281 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     8341 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     8401 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     8461 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     8521 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     8581 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     8641 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     8701 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     8761 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     8821 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     8881 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
     8941 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     9001 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     9061 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     9121 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     9181 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     9241 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     9301 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     9361 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     9421 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     9481 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     9541 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     9601 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
     9661 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
     9721 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
     9781 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
     9841 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
     9901 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
     9961 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    10021 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    10081 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    10141 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    10201 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    10261 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    10321 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    10381 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    10441 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    10501 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    10561 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    10621 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag
    10681 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    10741 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
    10801 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
    10861 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagcta agcttgagct ctcccatatg
    10921 gtcgactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
    10981 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
    11041 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
    11101 ggcctacgcg gcaggtctca tcaagacgat ctacccgagt aataatctcc aggagatcaa
    11161 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
    11221 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
    11281 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
    11341 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
    11401 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
    11461 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
    11521 agaccaaagg gctat
//
