LOCUS       sequence-255198-        2030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Starting substrate for in vitro DNA mismatch repair substrate 
            monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and 
            J.B. Hays 2002 JBC 277:26136..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P
  TITLE     In vitro studies of DNA mismatch repair proteins.
  JOURNAL   Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 
            10.1016/j.ab.2011.02.017. Epub 2011 Feb 15.
  PUBMED    21329650
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2011.02.017"; journalName: "Anal Biochem"; date: 
            "2011-06-15- 15"; volume: "413"; issue: "2"; pages: "179-84"
COMMENT     Sequence Label: pSCW02
FEATURES             Location/Qualifiers
     source          1..2030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(410..998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(490..509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1169..1960,1961..2029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1792..1811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcttccttt ttcaatctcg aggatctcgt gcacagctta ttattgaagc attgctgagg
       61 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      121 ttccgcgcac atttccccga aaagtgccac ctgacgtcat cgactcggat ccgactcatt
      181 cctgcagcga ctccatggga ctcaaataga caacgatttg aattcgctct tccgcttcct
      241 cgctcactgc ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtctta
      721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      901 gagttggtag ctcttggtcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      961 gcaagcagca gattacgcgc agaaaaaaag catctcaaga agattctttg atcttttcta
     1021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1081 caaaaaggat gttcacctag atgcttttaa attaaaaatg aagttttaaa tcaatctaaa
     1141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1201 cagcgatctg tctatttcgt tcatccatag ttgcctgact tcccgtcgtg tagataacta
     1261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1561 catggtcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1861 tctcaagaat cttaccgctg ttgaggtcca gttcgatgta acccactcgt gcacccaact
     1921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
//
