LOCUS       sequence-25520-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Chen G, DenBoer L, Shin J
  TITLE     Design of a single plasmid-based modified yeast one-hybrid system 
            for investigation of in vivo protein-protein and protein-DNA 
            interactions.
  JOURNAL   Biotechniques. 2008 Sep . 45(3):295-304.
  PUBMED    18778253
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2008 Sep . 45(3):295-304."
COMMENT     Sequence Label: pCETF
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(203..307)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(350..537)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     458..482
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aggncncatt ggnaacgttc ttcggggcga aactctcaag gatcttaccg ctgttgagat
       61 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      301 ttccgcgcac atttccccga aaagtgccac ctgacgtcaa gcttgcatgc cggtagaggt
      361 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      421 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      481 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      541 tttagaagtg tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat
      601 ttctggcaag gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa
      661 gaagtccaaa gcttctgaat aagccctcgt aatatcgntc nagannnttt cntgncccaa
      721 aaaanggggg nnnnttnctc ngncttaaaa atctcnaaag accgcngtnc ggggncaaga
      781 tgnancttna gggggttagt ncta
//
