LOCUS       sequence-255217-         367 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV 
            reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and
            13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, 
            Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
  TITLE     De novo-designed translation-repressing riboregulators for 
            multi-input cellular logic.
  JOURNAL   Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 
            10.1038/s41589-019-0388-1. Epub 2019 Nov 4.
  PUBMED    31686032
REFERENCE   2  (bases 1 to 367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0388-1"; journalName: "Nat Chem Biol"; date: 
            "2019-12"; volume: "15"; issue: "12"; pages: "1173-1182"
COMMENT     Sequence Label: pYZ_NAND4_L17_S24_S11_S19_S13
FEATURES             Location/Qualifiers
     source          1..367
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggctcctat cactttactt gttatagtta tgaacagagg agacataaca tgaacaagca
       61 cactaactac aaattcctaa ctttcaatcc agcatcaatc aattctactt gttatagtta
      121 tgaacagagg agacataaca tgaacaatca cataaaccta agaactaagc cgacaatact
      181 aacactaatc agatctactt gttatagtta tgaacagagg agacataaca tgaacaagca
      241 cctaacaaga ctaatcaact caatatcgca gatacaatca aatacaaatt gttatagtta
      301 tgaacagagg agacataaca tgaacaatca catacaaagc aaacgaaacc tggcggcagc
      361 gcaaaag
//
