LOCUS       sequence-25522-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Yao T, Song L, Xu W, DeMartino GN, Florens L, Swanson SK, Washburn 
            MP, Conaway RC, Conaway JW, Cohen RE
  TITLE     Proteasome recruitment and activation of the Uch37 deubiquitinating 
            enzyme by Adrm1.
  JOURNAL   Nat Cell Biol. 2006 Sep . 8(9):994-1002.
  PUBMED    16906146
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Sep . 8(9):994-1002."
COMMENT     Sequence Label: pcDNA3-HA-Uch37C88A
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             150..176
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgnggggnnt tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gcttggtacc gctagccgcc accatggctt
      121 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat
      181 ccatgacggg caacgccggg gagtggtgcc tcatggaaag cgaccccggg gtcttcaccg
      241 agctcattaa aggattcggt tgccgaggag cccaagtaga agaaatatgg agtttagagc
      301 ctgagaattt tgaaaaatta aagccagttc atgggttaat ttttcttttc aagtggcagc
      361 caggagaaga accagcaggc tctgtggttc aggactcccg acttgacacg atattttttg
      421 ctaagcaggt aattaataat gctgcagcta ctcaagccat agtgagtgtg ttactgaact
      481 gtacccacca ggatgtccat ttaggcgaga cattatcaga gtttaaagaa ttttcgcaaa
      541 gttttgatgc agctatgaaa ggcttggcac tgagcaattc agatgtgatt cgacaagtac
      601 acaacagttt cgccagacag caaatgctgg nttttanacg aagacatcac aaaaaactct
      661 tntctncnnn tnnnnnntnn nncngggngg gggnnttgtt nnttnngngn aaaagaagcc
      721 cgttnttngg cncacanaaa tgtggnccng cgccgccgca aaaaaaaaan anaaggcggg
      781 aggaattttt tttttggctn ggcgnaaang tttngnaaaa cnattcaact ccngnacnct
      841 ccctgttttt t
//
