LOCUS       sequence-255222-         182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of first-generation toehold repressor index 1 
            trigger RNA via the PluxB promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 182)
  AUTHORS   Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, 
            Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
  TITLE     De novo-designed translation-repressing riboregulators for 
            multi-input cellular logic.
  JOURNAL   Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 
            10.1038/s41589-019-0388-1. Epub 2019 Nov 4.
  PUBMED    31686032
REFERENCE   2  (bases 1 to 182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0388-1"; journalName: "Nat Chem Biol"; date: 
            "2019-12"; volume: "15"; issue: "12"; pages: "1173-1182"
COMMENT     Sequence Label: pAG_PluxB_ToeRep_N01_trigger
FEATURES             Location/Qualifiers
     source          1..182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      135..182
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttacagtcga ataaagggtc
       61 agacaaaccg gtaggtttgt ctgaatatat tacaacttcg cacttcatca catctatctt
      121 atcttcattt ctaactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      181 tg
//
