LOCUS       sequence-255223-         286 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of first-generation toehold repressor index 1 
            switch RNA with a GFP-ASV reporter via the Ptet* promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, 
            Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
  TITLE     De novo-designed translation-repressing riboregulators for 
            multi-input cellular logic.
  JOURNAL   Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 
            10.1038/s41589-019-0388-1. Epub 2019 Nov 4.
  PUBMED    31686032
REFERENCE   2  (bases 1 to 286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0388-1"; journalName: "Nat Chem Biol"; date: 
            "2019-12"; volume: "15"; issue: "12"; pages: "1173-1182"
COMMENT     Sequence Label: pAG_Ptet*_ToeRep_N01_switch
FEATURES             Location/Qualifiers
     source          1..286
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..41
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        79..128
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 ttttcagcag gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga
       61 tagagatact gagcacagct gtcaccggat gtgctttccg gtctgatgag tccgtgagga
      121 cgaaacagcc tctacaaata attttgttta aagaaatgaa gataagatag atgtgatgaa
      181 gtgcgaagtt gtaataacaa ataactatta ctacttcgct cttcatctca tctataaaca
      241 gaggagaata gatatgatga agagcaacct ggcggcagcg caaaag
//
