LOCUS       sequence-255270-       11949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: SUN2delCC
FEATURES             Location/Qualifiers
     source          1..11949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1189..1212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1216..1242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2492..2516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2888..3067
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3103..4128
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4326..4578
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4671..4695
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5216..6007
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6253..6841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6742..6761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6995..7012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7027..7167
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7093..7112)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7253..7275
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7511..7705
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7771..8844)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9273..9902)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11233..11257
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtgtcc
      481 aagggcgagg aggacaacat ggccatcatt aaggagttca tgcgcttcaa ggtccacatg
      541 gagggctccg ttaatgggca tgagttcgag atcgaggggg agggcgaggg gaggccctac
      601 gagggcacac agactgccaa gctgaaggtg acgaagggcg ggcctctccc cttcgcctgg
      661 gacatcctgt cgcctcagtt catgtacggc tctaaggcct acgtgaagca ccccgctgac
      721 attccagatt acctcaagct gagcttcccg gagggcttca agtgggagcg ggttatgaac
      781 ttcgaggacg gcggggtggt cacggtgacc caggactcca gcctccagga tggcgagttc
      841 atctacaagg tcaagctgcg cggcacaaat ttcccttccg acgggccggt tatgcagaag
      901 aagactatgg gctgggaggc ctcgtctgag cggatgtacc cggaggatgg cgcgctcaag
      961 ggggagatta agcagcgcct caagctgaag gacggtggcc actacgatgc tgaggtgaag
     1021 accacgtaca aggccaagaa gccggtccag ctcccaggcg cctacaacgt taatatcaag
     1081 ctggacatta catcgcacaa cgaggattac actatcgtgg agcagtacga gagggccgag
     1141 ggcaggcatt ctacgggcgg gatggacgag ctgtacaagg gcgccgggga ttacaaggac
     1201 gatgacgata aggcgtaccc ttacgacgtc cccgattacg cgggcgccgg gatggcttct
     1261 ccctcattcg cggccgctgc tacctctccg gcgtcatccc cactcacgct ggacgctatc
     1321 ccactggcct caaggcccgc cccaggcgcc gctgcggccc agcggaagcg ctcggtgttc
     1381 ctcctgaacc accggcctca tccggtctcg cccaccccgc ccctgcagcc aaccgctgcg
     1441 gcctccgctc agcacgccag gaggaagaag acctctcatc cacctaggcc caggtggcag
     1501 accgtgctct ccatcgctgc caagaatgcc gctctcctgg cggccctcct gtacctcggc
     1561 gacctggcct ggaggtggag ccacccgccc ccacctagcc cgcccccaga ccgggctgcc
     1621 ctggagggct acgcggctcg cgtcgatgag gttgaggcca gcctcacacg gactttccgc
     1681 atgatccagg tccagctgga ggcggttgac aggaagattg atggcgaagt gggggcggcg
     1741 cggggcgggg atgcgctcgc tggcctgaag aggatggagt tcctccggaa ggacgagttc
     1801 gagaagttct gggatgaggt gaagggctca ctgggctcct cctccgagtc ggaggtggac
     1861 ctcgatcagg tccgggccct ggcgcgcgag atcgtcatgc gcgagattga gaagcacgct
     1921 gcggacggca tcgggcgggt ggattacgct gtggcttccg gcgggggcag ggttgtgcgg
     1981 catagcgagg cctacgttcc caagaggggc ttcatggtgt ggatgtccgg cgtggacgtt
     2041 gggcccaagc cagagaagat gctgcagcct agcttcggcg agcccgggca gtgcttcgct
     2101 ctccagggct caaacgggtt cgtggaggtc aagctgaagt ccggcatcat tccagaggcg
     2161 gtgaccctgg agcacgtttc taaggacgtg gcctacgatc gctcaacggc gcctaagggc
     2221 tgcagggtct acgggtggta cgaggagacg cccggcgaga cccagtcggg gcatgccgct
     2281 aagatggcgc tcgctgagtt cgcctacgac ctggagaaga acaatgttca gacattcgac
     2341 gtgactgcgc cggatgttgg cgtgatcaac atggtgcgcc tcgacttcac atcgaatcac
     2401 ggctcatccc agctcacttg catctaccgc ctgcgggtgc acggccatga gcctgtcagc
     2461 cccggcagct cggctgggtc gcaggcctga gacccagctt tcttgtacaa agtggtgata
     2521 tcccgcggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc
     2581 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat
     2641 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     2701 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg
     2761 cgacgtcggg ccctctagag gatccccggg taccgcgaat tatcgatcat gagcggagaa
     2821 ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga
     2881 actgacagaa ccgcaacgtt gaaggagcca ctgagccgcg ggtttctgga gtttaatgag
     2941 ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca
     3001 gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat
     3061 ccaattagag tctcatattc actctcaact cgaatccccc ctatgaaaaa gcctgaactc
     3121 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3181 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3241 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3301 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt cagcgagagc
     3361 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3421 gaactgcccg ctgttcttca gccggtcgcg gaggctatgg atgctatcgc tgcggccgat
     3481 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3541 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3601 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3661 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3721 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3781 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3841 cgctacttcg agcggaggca tccggagctt gcaggatcgc caaggctccg ggcgtatatg
     3901 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     3961 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4021 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4081 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga
     4141 ccgaacaaga gctgatttcg agaacgcctc agccagcaac tcgcgcgagc ctagcaaggc
     4201 aaatgcgaga gaacggcctt acgcttggtg gcacagttct cgtccacagt tcgctaagct
     4261 cgctcggctg ggtcgcggga gggccggtcg cagtgattca ggaattaatt ccctagagtc
     4321 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     4381 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     4441 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     4501 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     4561 catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc gttaattcag
     4621 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     4681 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     4741 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     4801 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     4861 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     4921 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     4981 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     5041 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     5101 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     5161 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     5221 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     5281 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     5341 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     5401 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     5461 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     5521 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     5581 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     5641 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     5701 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     5761 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     5821 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     5881 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     5941 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     6001 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     6061 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     6121 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
     6181 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6361 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6901 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6961 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7021 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     7081 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     7141 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     7201 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7261 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     7321 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     7381 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     7441 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     7501 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     7561 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     7621 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     7681 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     7741 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     7801 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     7861 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     7921 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     7981 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     8041 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     8101 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     8161 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     8221 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     8281 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     8341 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     8401 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
     8461 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
     8521 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
     8581 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
     8641 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
     8701 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
     8761 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
     8821 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
     8881 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
     8941 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
     9001 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
     9061 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
     9121 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
     9181 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
     9241 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
     9301 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
     9361 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
     9421 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
     9481 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
     9541 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
     9601 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
     9661 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
     9721 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
     9781 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
     9841 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
     9901 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
     9961 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    10021 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    10081 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    10141 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    10201 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    10261 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    10321 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    10381 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    10441 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    10501 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    10561 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    10621 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    10681 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    10741 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    10801 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    10861 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    10921 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    10981 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    11041 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt aaagtacttt
    11101 aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
    11161 tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct
    11221 cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag
    11281 gcgggaaacg acaatctgat ccaagctcaa gctaagcttg agctctccca tatggtcgac
    11341 tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc tctatctcta
    11401 cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca ccgataatga
    11461 gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta
    11521 cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga tcaaatacct
    11581 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
    11641 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
    11701 tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc ctactgaatc
    11761 aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg tgaagactgg
    11821 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
    11881 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
    11941 aagggctat
//
