LOCUS       sequence-255271-       11580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: MLKP2del4
FEATURES             Location/Qualifiers
     source          1..11580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2123..2147)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2519..2698
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2734..3759
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3957..4209
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4302..4326
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4847..5638
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5884..6472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6373..6392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6626..6643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6658..6798
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6724..6743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6884..6906
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7142..7336
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7402..8475)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8904..9533)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10864..10888
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt aatggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccgatcctgg tggagctgga cggggatgtc
      541 aacggccaca agttctcagt gtccggcgag ggggagggcg acgcgacata cgggaagctc
      601 actctgaagt tcatttgcac gaccggcaag ctcccggttc cctggccaac cctggtgaca
      661 actctcacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc agagggctac gtgcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagaccag ggcggaggtg aagttcgagg gggacacgct ggtcaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctgggcca caagctggag
      901 tacaactaca attcgcataa cgtttacatc atggctgaca agcagaagaa cggcattaag
      961 gtcaatttca agatccgcca caatattgag gacggctccg tccagctggc cgatcattac
     1021 cagcagaaca caccgatcgg ggacggccct gtcctcctgc ccgataatca ctacctgtct
     1081 actcagtcag ccctctccaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacgg cggccgggat caccctgggc atggacgagc tgtacaaggg ggccggcgat
     1201 tacaaggacg atgacgataa ggcgtacccc tacgacgtcc cagattacgc gggggctggc
     1261 atgctcatgc actacgacgt ggataggtcg gaggacaggc agtccgagga ggtccagtct
     1321 gcgtaccgca gggacagcga tatcggcgag gagatgatca tttccagcga ggagcagaac
     1381 gacgagaatg agtggtcgtg ggtgaaccag gacagggatg gtggcccact ggctgagtct
     1441 gtttcgtctc tccacatgac ccagcaggtg ctggagaatg agatccagaa gctctcggag
     1501 ctggggaagg agctggaggc cgaggagagc acgtcgggca acaaggacca ggatgtcatt
     1561 gttctgccat acgctgaggt ggacatgctc aagctgaatg agaagatgga gcacctggag
     1621 cagaaggtga aggaggtctc taacacaatc agggagaagg acctccggct gtcaaagctc
     1681 cagatcctga ttggcacagc ggacagggcc actgtggagg aggctgctgc gagcattgat
     1741 cagctggaga ccgagttcga gcaccatctg caggagatcc tggaggctga gattcagtac
     1801 ctcgtcatga tgaaggcccg ccagaattgg caggttaggg cggaggacca gatggctctg
     1861 gaggagcaca gggcctcagc tgcgggcgat aacacacgga tgctcctgaa gctccgcgag
     1921 actgagagca agatcgttat gctcaaggag caggtggaca agctggaggt tagggagaag
     1981 gatctctacc ggaccacgga ggtgctgaag atgcagtcac gcaccttcaa ggtctccctc
     2041 ttcggcctgg ttcagctcat catgatgtgc ctcagcctga agatgttctt cgctcaggtg
     2101 tcggcccctt tcgacgagtg aaacccagct ttcttgtaca aagtggtgat atcccgcggc
     2161 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
     2221 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     2281 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     2341 tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg
     2401 gccctctaga ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga attaagggag
     2461 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga
     2521 accgcaacgt tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca
     2581 tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa
     2641 tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga
     2701 gtctcatatt cactctcaac tcgaatcccc cctatgaaaa agcctgaact caccgcgacg
     2761 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     2821 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     2881 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     2941 gccgcgctcc cgattccgga agtgcttgac attggggagt tcagcgagag cctgacctat
     3001 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     3061 gctgttcttc agccggtcgc ggaggctatg gatgctatcg ctgcggccga tcttagccag
     3121 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     3181 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     3241 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     3301 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     3361 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     3421 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     3481 gagcggaggc atccggagct tgcaggatcg ccaaggctcc gggcgtatat gctccgcatt
     3541 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     3601 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     3661 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     3721 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag
     3781 agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag
     3841 agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct
     3901 gggtcgcggg agggccggtc gcagtgattc aggaattaat tccctagagt caagcagatc
     3961 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     4021 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     4081 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     4141 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     4201 tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca gtacattaaa
     4261 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     4321 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     4381 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     4441 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     4501 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     4561 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     4621 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     4681 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     4741 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     4801 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     4861 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     4921 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     4981 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     5041 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     5101 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     5161 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     5221 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     5281 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     5341 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     5401 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     5461 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     5521 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     5581 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     5641 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     5701 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     5761 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     5821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6001 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     6661 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     6721 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     6781 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6841 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     6901 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     6961 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     7021 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     7081 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     7141 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     7201 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     7261 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     7321 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     7381 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     7441 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     7501 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     7561 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     7621 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     7681 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     7741 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     7801 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     7861 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     7921 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     7981 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     8041 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     8101 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     8161 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     8221 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     8281 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     8341 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     8401 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     8461 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     8521 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     8581 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     8641 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     8701 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     8761 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     8821 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     8881 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     8941 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     9001 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     9061 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     9121 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     9181 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     9241 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     9301 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     9361 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     9421 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     9481 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     9541 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     9601 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     9661 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     9721 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     9781 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     9841 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     9901 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     9961 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    10021 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    10081 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    10141 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    10201 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    10261 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    10321 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    10381 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    10441 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    10501 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    10561 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    10621 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    10681 gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt taaagtactt
    10741 taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    10801 ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct
    10861 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac
    10921 gacaatctga tccaagctca agctaagctt gagctctccc atatggtcga ctagagccaa
    10981 gctgatctcc tttgccccgg agatcaccat ggacgacttt ctctatctct acgatctagg
    11041 aagaaagttc gacggagaag gtgacgatac catgttcacc accgataatg agaagattag
    11101 cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct acgcggcagg
    11161 tctcatcaag acgatctacc cgagtaataa tctccaggag atcaaatacc ttcccaagaa
    11221 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
    11281 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcataaacc
    11341 aaggcaagta atagagattg gagtctctaa gaaagtagtt cctactgaat caaaggccat
    11401 ggagtcaaaa attcagatcg aggatctaac agaactcgcc gtgaagactg gcgaacagtt
    11461 catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
    11521 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
//
