LOCUS       sequence-255274-       11571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: MLKP2delKASH
FEATURES             Location/Qualifiers
     source          1..11571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2114..2138)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2510..2689
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2725..3750
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3948..4200
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4293..4317
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4838..5629
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5875..6463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6364..6383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6617..6634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6649..6789
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6715..6734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6875..6897
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7133..7327
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7393..8466)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8895..9524)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10855..10879
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt aatggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccgatcctgg tggagctgga cggggatgtc
      541 aacggccaca agttctcagt gtccggcgag ggggagggcg acgcgacata cgggaagctc
      601 actctgaagt tcatttgcac gaccggcaag ctcccggttc cctggccaac cctggtgaca
      661 actctcacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc agagggctac gtgcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagaccag ggcggaggtg aagttcgagg gggacacgct ggtcaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctgggcca caagctggag
      901 tacaactaca attcgcataa cgtttacatc atggctgaca agcagaagaa cggcattaag
      961 gtcaatttca agatccgcca caatattgag gacggctccg tccagctggc cgatcattac
     1021 cagcagaaca caccgatcgg ggacggccct gtcctcctgc ccgataatca ctacctgtct
     1081 actcagtcag ccctctccaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacgg cggccgggat caccctgggc atggacgagc tgtacaaggg ggccggcgat
     1201 tacaaggacg atgacgataa ggcgtacccc tacgacgtcc cagattacgc gggggctggc
     1261 atgctcatgc actacgacgt ggataggtcg gaggacaggc agtccgagga ggtccagtct
     1321 gcgtaccgca gggacagcga tatcggcgag gagatgatca tttccagcga ggagcagaac
     1381 gacgagaatg agtggtcgtg ggtgaaccag gacagggatg gtggcccact ggctgagtct
     1441 gtttcgtctc tccacatgac ccagcaggtg ctggagaatg agatccagaa gctctcggag
     1501 ctggggaagg agctggaggc cgaggagagc acgtcgggca acaaggacca ggatgtcatt
     1561 gttctgccat acgctgaggt ggacatgctc aagctgaatg agaagatgga gcacctggag
     1621 cagaaggtga aggaggtctc taacacaatc agggagaagg acctccggct gtcaaagctc
     1681 cagatcctga ttggcacagc ggacagggcc actgtggagg aggctgctgc gagcattgat
     1741 cagctggaga ccgagttcga gcaccatctg caggagatcc tggaggctga gattcagtac
     1801 ctcgtcatga tgaaggcccg ccagaattgg caggttaggg cggaggacca gatggctctg
     1861 gaggagcaca gggcctcagc tgcgggcgat aacacacgga tgctcctgaa gctccgcgag
     1921 actgagagca agatcgttat gctcaaggag caggtggaca agctggaggt tagggagaag
     1981 gatctctacc ggaccacgga ggtgctgaag atgcagtcac gcaccttcaa ggtctccctc
     2041 ttcggcctgg ttcagctcat catgatgtgc ctcagcctga agatgttctt cgctcaggtg
     2101 gctgccgcat gaaacccagc tttcttgtac aaagtggtga tatcccgcgg ccatgctaga
     2161 gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat
     2221 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt
     2281 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     2341 tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg ggccctctag
     2401 aggatccccg ggtaccgcga attatcgatc atgagcggag aattaaggga gtcacgttat
     2461 gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag aaccgcaacg
     2521 ttgaaggagc cactgagccg cgggtttctg gagtttaatg agctaagcac atacgtcaga
     2581 aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatttcttg
     2641 tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag agtctcatat
     2701 tcactctcaa ctcgaatccc ccctatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     2761 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     2821 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     2881 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     2941 ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc
     3001 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctt
     3061 cagccggtcg cggaggctat ggatgctatc gctgcggccg atcttagcca gacgagcggg
     3121 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     3181 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     3241 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     3301 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     3361 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     3421 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     3481 catccggagc ttgcaggatc gccaaggctc cgggcgtata tgctccgcat tggtcttgac
     3541 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     3601 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     3661 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     3721 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt
     3781 cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc
     3841 ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg
     3901 gagggccggt cgcagtgatt caggaattaa ttccctagag tcaagcagat cgttcaaaca
     3961 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4021 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4081 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4141 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4201 gaccggcatg caagctgata attcaattcg gcgttaattc agtacattaa aaacgtccgc
     4261 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     4321 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     4381 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     4441 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     4501 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     4561 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     4621 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     4681 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     4741 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     4801 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     4861 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     4921 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     4981 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     5041 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     5101 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     5161 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     5221 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     5281 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     5341 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     5401 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     5461 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     5521 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     5581 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     5641 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     5701 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     5761 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     5821 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5881 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5941 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6001 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6061 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6121 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6181 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6241 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6301 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6361 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6421 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6481 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6541 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6601 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     6661 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     6721 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     6781 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6841 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6901 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     6961 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     7021 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     7081 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     7141 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     7201 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     7261 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     7321 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     7381 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     7441 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     7501 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     7561 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     7621 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     7681 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     7741 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     7801 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     7861 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     7921 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     7981 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     8041 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     8101 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     8161 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     8221 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     8281 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     8341 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     8401 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     8461 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     8521 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     8581 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     8641 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     8701 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     8761 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     8821 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     8881 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     8941 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     9001 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     9061 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     9121 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     9181 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     9241 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     9301 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     9361 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     9421 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     9481 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     9541 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     9601 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     9661 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     9721 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     9781 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     9841 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     9901 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     9961 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    10021 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    10081 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    10141 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    10201 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    10261 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    10321 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    10381 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    10441 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    10501 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    10561 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    10621 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    10681 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
    10741 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    10801 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
    10861 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
    10921 atccaagctc aagctaagct tgagctctcc catatggtcg actagagcca agctgatctc
    10981 ctttgccccg gagatcacca tggacgactt tctctatctc tacgatctag gaagaaagtt
    11041 cgacggagaa ggtgacgata ccatgttcac caccgataat gagaagatta gcctcttcaa
    11101 tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcggcag gtctcatcaa
    11161 gacgatctac ccgagtaata atctccagga gatcaaatac cttcccaaga aggttaaaga
    11221 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa
    11281 gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac caaggcaagt
    11341 aatagagatt ggagtctcta agaaagtagt tcctactgaa tcaaaggcca tggagtcaaa
    11401 aattcagatc gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag
    11461 tcttttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct
    11521 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta t
//
