LOCUS       sequence-255302-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for multivalent scaffold of 
            (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing 
            client proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK
  TITLE     Compositional Control of Phase-Separated Cellular Bodies.
  JOURNAL   Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. 
            Epub 2016 Jun 30.
  PUBMED    27374333
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.06.010"; journalName: "Cell"; date: 
            "2016-07-28- 28"; volume: "166"; issue: "3"; pages: "651-663"
COMMENT     Sequence Label: (SUMO)5-(SIM)10
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1101
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      958..967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1075..1098
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1150..1161
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1174..1194
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3823..3843
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3844..3861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3874..3891)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3874..3890)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3883..3905)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4069..4086)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4239..4325
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4417..4444
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4463..4554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4555..4623,4624..5415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4773..4792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5457..5970)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5652..5673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5864..5883
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6081..6669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6570..6589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6823..6840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6946..6965)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7106..7128
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7122..7313)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7809..7886
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7887..8969
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7906..7925)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(9212..9217,9218..9233,9234..9240)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    9248..9264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9253..9275
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaaaactg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt
       61 ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat
      121 ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt
      181 atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc
      241 accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac
      301 aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa
      361 gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg
      421 aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg
      481 ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa
      541 gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt
      601 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
      661 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
      721 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt
      781 ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc
      841 ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg
      901 ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc
      961 accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
     1021 tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
     1081 gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac
     1141 aacctcggga tcgagggaag gatttcagaa tttgaaaacc tgtattttca gggccatatg
     1201 ggtgggagtt ggggagggtc tatgtcagaa gagaagccta aggagggcgt gaaaaccgag
     1261 aatgaccaca ttaacttgaa ggttgctgga caggatgggt ccgtggttca gttcaaaatt
     1321 aaacgccaca caccgttgtc gaaactgatg aaagcgtaca gcgaacgcca gggtctttct
     1381 atgcgtcaga ttcgcttccg ctttgatggt caaccgatta atgaaacgga cacgccggca
     1441 cagctggaga tggaggatga agacactatc gacgtatttc agcagcagac cgtcgtaggg
     1501 ggctccggcg gaagcggggg gtcgggaggt tccatgagcg aagagaaacc aaaagaagga
     1561 gttaaaaccg aaaacgacca tatcaacctg aaggtcgccg gtcaggacgg ctcagtcgtg
     1621 cagttcaaga ttaaacgtca taccccatta agtaagctga tgaaggcgta tagtgagcgc
     1681 caaggcttgt cgatgcgcca gattcgtttt cgctttgatg gtcagccaat caacgaaacc
     1741 gacaccccgg cgcagctgga aatggaagat gaagatacca tcgatgtgtt ccaacagcaa
     1801 acggttgttg gcggaagcgg cgggagcggt ggtagcgggg gttctatgtc cgaagaaaaa
     1861 ccgaaagagg gtgtgaagac tgaaaacgac cacattaatc tgaaggtggc gggccaggac
     1921 ggttccgttg ttcagtttaa aatcaaacgt cacaccccgc tgtcgaaact gatgaaagcc
     1981 tatagcgaac gtcaagggct gagcatgcgt caaattcgtt ttcgctttga tggtcagcca
     2041 atcaacgaaa ccgatacacc ggcgcagctg gagatggaag acgaggacac cattgacgtc
     2101 ttccagcaac aaactgtagt gggtggctcc ggtggctctg gtgggtctgg tggctcgatg
     2161 agtgaggaaa aacccaaaga aggcgtcaag actgagaacg atcatattaa cctgaaagtc
     2221 gcgggtcaag atggttcagt ggtgcagttc aaaattaaac gccacacccc gctgtcaaag
     2281 ctgatgaaag cctatagtga gcgtcaaggt ctgtcgatgc gccagatccg ctttcgcttt
     2341 gatggtcagc caatcaacga gacagacact ccggcacaac tggagatgga agatgaagac
     2401 accatcgatg tttttcaaca gcagaccgtg gtgggaggga gcggcggtag cgggggatca
     2461 ggtggtagta tgtctgagga aaagccgaag gaaggggtaa agaccgaaaa tgatcacatt
     2521 aatctgaaag ttgcgggcca agatggtagt gtagtgcaat ttaaaatcaa gcgtcacacc
     2581 ccgttatcta aactgatgaa agcatatagc gaacgccaag gcctgtctat gcgccagatt
     2641 cgctttcgct ttgacgggca accaattaat gaaacggaca ccccggcaca actggaaatg
     2701 gaagacgagg atactattga cgtgtttcag caacagacag tggtcggcgg ctccggtgga
     2761 tctggtggct cttggggtgg gagcaaagta gatgtgatcg acttgaccat cgaaagcagc
     2821 agcgacgaag aagaagatcc gcctgccaaa cgcggcggta gcggtggctc tggcgggagc
     2881 ggcggctcga aagttgatgt cattgacctt acaattgaat cttcgagcga tgaagaggaa
     2941 gatcctccag cgaaacgtgg cggctcggga ggttcgggcg gcagcggcgg aagcaaagtc
     3001 gatgtaattg acttaacgat cgaatctagc agcgatgaag aagaagatcc accggctaaa
     3061 cgtgggggct cagggggttc tggcggttca ggcggcagta aagttgacgt gattgacctc
     3121 accatcgaaa gcagtagcga cgaggaagaa gatccaccag caaaacgcgg agggagcggg
     3181 ggcagcggcg gcagcggcgg atctaaagtg gacgttatcg atctgacgat cgaaagcagt
     3241 agcgatgagg aagaggaccc gccgacgaaa cggggcggct cggggggatc cggtggctct
     3301 tggggtggga gcaaagtaga tgtgatcgac ttgaccatcg aaagcagcag cgacgaagaa
     3361 gaagatccgc ctgccaaacg cggcggtagc ggtggctctg gcgggagcgg cggctcgaaa
     3421 gttgatgtca ttgaccttac aattgaatct tcgagcgatg aagaggaaga tcctccagcg
     3481 aaacgtggcg gctcgggagg ttcgggcggc agcggcggaa gcaaagtcga tgtaattgac
     3541 ttaacgatcg aatctagcag cgatgaagaa gaagatccac cggctaaacg tgggggctca
     3601 gggggttctg gcggttcagg cggcagtaaa gttgacgtga ttgacctcac catcgaaagc
     3661 agtagcgacg aggaagaaga tccaccagca aaacgcggag ggagcggggg cagcggcggc
     3721 agcggcggat ctaaagtgga cgttatcgat ctgacgatcg aaagcagtag cgatgaggaa
     3781 gaggacccgc cggcgaaacg gggcggctcg tgcgggagat ccgaaaacct gtattttcag
     3841 ggccatcatc atcatcatca ctgaaagctt ggcactggcc gtcgttttac aacgtcgtga
     3901 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3961 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4021 tggcgaatgg cagcttggct gttttggcgg atgagataag attttcagcc tgatacagat
     4081 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     4141 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     4201 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     4261 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     4321 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     4381 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     4441 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
     4501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4741 cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     4801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaccc
     5461 cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg
     5521 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     5581 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagggt
     5641 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     5701 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa
     5761 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     5821 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     5881 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     5941 ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat cctttttgat
     6001 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6841 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     6901 cgcatgtggt gcactctcag tacaccgcat gtggtgcact ctcagtacaa tctgctctga
     6961 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     7021 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     7081 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     7141 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg cagcgattca
     7201 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     7261 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     7321 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     7381 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     7441 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     7501 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     7561 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     7621 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     7681 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     7741 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     7801 gaaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg
     7861 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
     7921 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
     7981 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     8041 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
     8101 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
     8161 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     8221 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     8281 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
     8341 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
     8401 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
     8461 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
     8521 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
     8581 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
     8641 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
     8701 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
     8761 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     8821 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     8881 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8941 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact
     9001 cattaggcac aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt
     9061 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat
     9121 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
     9181 acggttctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg
     9241 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt
     9301 tcacgagcac ttcaccaaca aggaccatag att
//
