LOCUS       sequence-255312-         116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes short hairpin RNA (shRNA) #2 that targets the 
            3'-untranslated region of the rat Nts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 116)
  AUTHORS   Pomrenze MB, Giovanetti SM, Maiya R, Gordon AG, Kreeger LJ, Messing 
            RO
  TITLE     Dissecting the Roles of GABA and Neuropeptides from Rat Central 
            Amygdala CRF Neurons in Anxiety and Fear Learning.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi: 
            10.1016/j.celrep.2019.08.083.
  PUBMED    31577943
REFERENCE   2  (bases 1 to 116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "13-21.e4"
COMMENT     Sequence Label: pPRIME-CMV-GFP-shNts2
FEATURES             Location/Qualifiers
     source          1..116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           58..72
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ctcgagaagg tatattgctg ttgacagtga gcgcacctgt tatctggata cacattagtg
       61 aagccacaga tgtaatgtgt atccagataa caggtatgcc tactgcctcg gaattc
//
