LOCUS       sequence-255318-        3260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an iconic representation of a house cat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Banal JL, Shepherd TR, Berleant J, Huang H, Reyes M, Ackerman CM, 
            Blainey PC, Bathe M
  TITLE     Random access DNA memory using Boolean search in an archival file 
            storage system.
  JOURNAL   Nat Mater. 2021 Sep;20(9):1272-1280. doi: 
            10.1038/s41563-021-01021-3. Epub 2021 Jun 10.
  PUBMED    34112975
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41563-021-01021-3"; journalName: "Nat Mater"; date: 
            "2021-09"; volume: "20"; issue: "9"; pages: "1272-1280"
COMMENT     Sequence Label: pUCIDT-Cat2
FEATURES             Location/Qualifiers
     source          1..3260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1039..1055)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1039..1055)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1052..1074)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1063..1079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1087..1093,1094..1111,1112..1117))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1132..1153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1270..1287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1441..2029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1521..1540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2200..2991,2992..3060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2823..2842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3233..3251
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atgaattcgt cgtcgtcccc tcaaactatc atatctctag acgtgtcgcg aagtcgacac
      541 agcgtacacg ctgtcggtta caacgcagtg agctaacata tgcagatcac gtttgacggg
      601 agtacattat aaactcaccg cgagcttagt ctcgagctcg ccgtggagat agatagtgtg
      661 ccagcatcga cgcgtccgtc ttcgtcagca gtaacgcgct cgaaaccagt ctgagacacg
      721 aaatcgtata tctgtcatat tcgtccgatg ggctattgga ccggacagct ctcgctcgaa
      781 cccgcccgtg ctaaaaattt gcggactatt ttaaacaaga gtcatcctgt tctactacag
      841 tgaagccctg ttgatgggcg cgtgctggag agtagtacgg atgacaacat taacgacgag
      901 gccggtacga gatatactag gaatcgaggt cgtcccgagc atcgctgatg acgacgtgtc
      961 agtggctgaa aaggtggcat caatctgcag atcagttctg gaccagcgag ctgtgctgcg
     1021 actcgtggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1201 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1561 ctctcctgtt ccgaccctgt cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actctacctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3181 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3241 tatcacgagg ccctttcgtc
//
