LOCUS       sequence-255324-        3187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an iconic representation of a portrait of George Washington.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Banal JL, Shepherd TR, Berleant J, Huang H, Reyes M, Ackerman CM, 
            Blainey PC, Bathe M
  TITLE     Random access DNA memory using Boolean search in an archival file 
            storage system.
  JOURNAL   Nat Mater. 2021 Sep;20(9):1272-1280. doi: 
            10.1038/s41563-021-01021-3. Epub 2021 Jun 10.
  PUBMED    34112975
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41563-021-01021-3"; journalName: "Nat Mater"; date: 
            "2021-09"; volume: "20"; issue: "9"; pages: "1272-1280"
COMMENT     Sequence Label: pUCIDT-Washington
FEATURES             Location/Qualifiers
     source          1..3187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(966..982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(966..982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(979..1001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    990..1006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1014..1020,1021..1038,1039..1044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1059..1080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1197..1214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1368..1956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1448..1467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2127..2918,2919..2987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2750..2769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2988..3092)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3160..3178
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atgaattcgt cgtcgtcccc tcaaactatc atatcgctcg ctacgtcaga ttatgagcag
      541 tcatcacgac agatgtccat tccaatggta cacatagtgt gtcctagatc gacagtattc
      601 gcggcgagta ttatttagcg gctctgtttc agcaccggaa caataaggac gaatagtata
      661 catatgccta atacgttttc acgtcggcac aactcataga gaatatccgg gatgacgcgt
      721 gacaatcagt gatcaatgta agcttttaac cgattaggta gcgtcgtggt taaccgagac
      781 tgtgagttac tcctatgttc tgcaatagcc gaggtcacct ttgttcatca actgggcggc
      841 caatcgtaaa tcctgcttat agctaggatc atcgacgcga gagctatcta ggctgaaaag
      901 gtggcatcaa tctgcagatc agttctggac cagcgagctg tgctgcgact cgtggcgtaa
      961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1501 accctgtcgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact ctaccttttt
     3001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3121 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3181 tttcgtc
//
