LOCUS       sequence-255326-        3190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an iconic representation of a skyscraper.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Banal JL, Shepherd TR, Berleant J, Huang H, Reyes M, Ackerman CM, 
            Blainey PC, Bathe M
  TITLE     Random access DNA memory using Boolean search in an archival file 
            storage system.
  JOURNAL   Nat Mater. 2021 Sep;20(9):1272-1280. doi: 
            10.1038/s41563-021-01021-3. Epub 2021 Jun 10.
  PUBMED    34112975
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41563-021-01021-3"; journalName: "Nat Mater"; date: 
            "2021-09"; volume: "20"; issue: "9"; pages: "1272-1280"
COMMENT     Sequence Label: pUCIDT-Skyscraper1
FEATURES             Location/Qualifiers
     source          1..3190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(969..985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(969..985)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(982..1004)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    993..1009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1017..1023,1024..1041,1042..1047))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1062..1083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1200..1217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1451..1470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2130..2921,2922..2990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2753..2772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2991..3095)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3163..3181
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atgaattcgt cgtcgtcccc tcaaactatc atatctcagc tcatctacta gacatatgca
      541 tcgtgtcaga tgcgttgtcc caggactatg agtaggtgtt atcactacgc acaccaactt
      601 gtggtagcgg gtaagacata gacgcggatc ctcctcggcc tacgtaaaaa ttctcgctgc
      661 gtgacttaag actcctcgca agacggagat ctcgccgttg ccggaagaag ccgccgaccg
      721 tggtggaaag gtcatcgtta ctaacgaggc aggcgcaaga tgcgtgtccg taggccgagt
      781 ggataatgac cgtgcgagtg atctacagtg tcatagcggt actctgtacc ggcggttccc
      841 gattggtaag ttaccaccat tttgtacact atcgtcagcg tagcatgcgc tatagctgaa
      901 aaggtggcat caatctgcag atcagttctg gaccagcgag ctgtgctgcg actcgtggcg
      961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1021 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1081 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1141 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgt cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actctacctt
     3001 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3121 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3181 ccctttcgtc
//
