LOCUS       sequence-255338-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a donor vector for recombination with the Bxb1 recombinase 
            with variant G315T of the Beta-2 Adrenergic Receptor and a 
            cAMP-responsive luciferase reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Jones EM, Lubock NB, Venkatakrishnan AJ, Wang J, Tseng AM, Paggi JM,
            Latorraca NR, Cancilla D, Satyadi M, Davis JE, Babu MM, Dror RO, 
            Kosuri S
  TITLE     Structural and functional characterization of G protein-coupled 
            receptors with deep mutational scanning.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 54895. doi: 10.7554/eLife.54895.
  PUBMED    33084570
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 54895. doi"; pages: " 
            10.7554/eLife.54895"
COMMENT     Sequence Label: Bxb1Donor_G315T_ADRB2_LucReporter
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..51
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             87..152
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tgcacaagct tccggcttgt cgacgacggc ggtctccgtc gtcaggatca tccggggtag
       61 taataagggc gaccgccgcc accatggact acaaagacca tgacggtgat tataaagatc
      121 atgacatcga ttacaaggat gacgatgata aaggatccgg ccagcctggc aatggcagcg
      181 cctttctgct ggcccctaat ggctctcacg cccccgatca cgacgtgacc caggaacggg
      241 atgaagtgtg ggtcgtgggc atgggcatcg tgatgagcct gatcgtgctg gccatcgtgt
      301 tcggcaacgt gctcgtgatc accgctatcg ccaagttcga gcggctgcag accgtgacca
      361 actacttcat cacctccctg gcctgcgccg acctcgtgat gggactggct gtggtgcctt
      421 ttggagccgc ccacatcctg atgaagatgt ggaccttcgg caacttttgg tgcgagttct
      481 ggaccagcat cgacgtgctg tgcgtgaccg cctccatcga gacactgtgt gtgatcgccg
      541 tggacagata cttcgccatc accagcccct tcaagtacca gagcctgctg accaagaaca
      601 aggccagagt gatcattctg atggtgtgga tcgtgtccgg cctgaccagc ttcctgccca
      661 tccagatgca ctggtacaga gccacccacc aggaagccat caactgctac gccaacgaaa
      721 cctgctgcga cttcttcaca aaccaggcct acgccattgc ctccagcatc gtgtccttct
      781 acgtgcccct cgtgattatg gtgttcgtgt acagccgggt gttccaggaa gctaagcggc
      841 agctgcagaa gatcgacaag agcgagggcc ggttccacgt gcagaacctg agccaggtgg
      901 aacaggacgg cagaacaggc cacggcctgc gganaagcag caagtnctgc ctgaaagagc
      961 acaanncctg aaaaccctnn natcatcatg ggcacntcac cctgngctgg ctgncctncn
     1021 tcatcnngan atcnngcacg tgatccanna nanctgatcc ggaannngnt
//
