LOCUS       sequence-255339-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a donor vector for recombination with the Bxb1 recombinase 
            with variant G315T of the Beta-2 Adrenergic Receptor and a 
            cAMP-responsive luciferase reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Jones EM, Lubock NB, Venkatakrishnan AJ, Wang J, Tseng AM, Paggi JM,
            Latorraca NR, Cancilla D, Satyadi M, Davis JE, Babu MM, Dror RO, 
            Kosuri S
  TITLE     Structural and functional characterization of G protein-coupled 
            receptors with deep mutational scanning.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 54895. doi: 10.7554/eLife.54895.
  PUBMED    33084570
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 54895. doi"; pages: " 
            10.7554/eLife.54895"
COMMENT     Sequence Label: Bxb1Donor_G315T_ADRB2_LucReporter
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(594..611)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     821..840
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctgggcatca tcatgggcac cttcaccctg tgctggctgc ccttcttcat cgtgaacatc
       61 gtgcacgtga tccaggacaa cctgatccgg aaagaggtgt acatcctgct gaactggatc
      121 acatacgtga acagcggctt caaccccctg atctactgca gaagccccga cttccggatc
      181 gccttccagg aactgctgtg cctgagaaga agcagcctga aggcctacgg caacggctac
      241 agcagcaacg gcaataccgg cgagcagagc ggctaccatg tggaacagga aaaagaaaac
      301 aagctgctgt gcgaggacct gcccggcacc gaggattttg tgggccacca gggaaccgtg
      361 cccagcgaca acatcgacag ccagggcaga aactgcagca ccaacgacag cctgctgtga
      421 gctagccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
      481 ccggtgtgcg tttgtctata tgtgattttc caccatattg ccgtcttttg gcaatgtgag
      541 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
      601 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
      661 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
      721 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
      781 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
      841 caacnaaggg gctgaaggat gcccagaaag gtaccccatt gtatgggatc tgatctgggg
      901 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct nnncccccga
      961 accacggngn cntggntttc ctttnaaaan ncgatgatan ntgaaaancc ttcannatct
     1021 ctcancagnn tctnganct
//
