LOCUS       sequence-25536-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, 
            Florens L, Conaway RC, Cohen RE, Conaway JW
  TITLE     Distinct modes of regulation of the Uch37 deubiquitinating enzyme in
            the proteasome and in the Ino80 chromatin-remodeling complex.
  JOURNAL   Mol Cell. 2008 Sep 26. 31(6):909-17.
  PUBMED    18922472
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Sep 26. 31(6):909-17."
COMMENT     Sequence Label: pET19b NFRKB 1-101
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(361..375)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(391..420)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     RBS             433..455
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    470..494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(494..513)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(495..513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(597..616)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctcttngggc ttgttgcagc cggatccgcc tgcagcggta cctcatccaa agcggaagtt
       61 ctccccactg aacaaggcta agatgagttc attctgctgc tcagcactgt cttcaggaaa
      121 ctggggcaga aactgctgga ggtgttcacg ttgagaatca cttaacactt cctgccatgt
      181 tgagaggctg acaacatcaa agaagatctc aggatcctcc agaaggtcct cgggcagact
      241 aactctggtg cctcccagga ggcaatcctc catgatgcgc gtgccatggc catctccaca
      301 cggaccaagt tccagaggat ctgtcagcat atggtctaag gaatccatgt cgagcatatg
      361 cttgtcgtcg tcgtcgatat ggccgctgct gtgatgatga tgatgatgat gatgatgatg
      421 gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      481 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      541 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      601 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      661 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      721 gcatgcacca tttgnnnnnn ggggccgccc cnnnnnnnnc cncnnggggg ggnncntncn
      781 nngggggatc ccnaanggna ancnncna
//
