LOCUS       sequence-25540-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, 
            Florens L, Conaway RC, Cohen RE, Conaway JW
  TITLE     Distinct modes of regulation of the Uch37 deubiquitinating enzyme in
            the proteasome and in the Ino80 chromatin-remodeling complex.
  JOURNAL   Mol Cell. 2008 Sep 26. 31(6):909-17.
  PUBMED    18922472
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Sep 26. 31(6):909-17."
COMMENT     Sequence Label: pcDNA5 Flag-NFRKB delta101
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     70..94
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             176..199
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             206..232
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cnnggngnat tagcgagctc tccctatcag tgatagagat ctccctatca gtgatagaga
       61 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
      121 tgacctccat agaagacacc gggaccgatc cagcctccgg actgctagca tgccagacta
      181 caaggacgat gatgacaagc ttaagtaccc atacgatgtt ccagattacg ctctcgacaa
      241 ccctctgcac attgcccaga agcttttccg agacggacac tttaaccccg aggtggtcaa
      301 gtaccggcag ttatgcttca agtcacagta caagcgctac ctcaactccc agcagcagta
      361 tttccatcgg ctgctgaagc aaattcttgc ttcccggagt gatctgctgg agatggcccg
      421 gcggagtggc cccgcccttc ccttccggca gaaacgccct tcaccatccc gcacacctga
      481 ggagcgggag tggcggaccc agcagcgcta cttgaaggtc ttaagggaag tgaaagagga
      541 gtgtggtgac acagccctgt catctgatga agaggatctc agctcatggc ttccgagctc
      601 tccagcacgt tctcctagtc ctgcgggcct gcnnnattgt atcnnaaaga
//
